Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5016 and Pf1N1B4_5017 are separated by 140 nucleotides Pf1N1B4_5017 and Pf1N1B4_5018 are separated by 177 nucleotides Pf1N1B4_5018 and Pf1N1B4_5019 are separated by 72 nucleotides Pf1N1B4_5019 and Pf1N1B4_5020 are separated by 212 nucleotides Pf1N1B4_5020 and Pf1N1B4_5021 are separated by 22 nucleotides
Pf1N1B4_5016 - hypothetical protein, at 5,567,715 to 5,567,903
_5016
Pf1N1B4_5017 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,568,044 to 5,568,325
_5017
Pf1N1B4_5018 - FIG00962345: hypothetical protein, at 5,568,503 to 5,568,733
_5018
Pf1N1B4_5019 - FIG00958773: hypothetical protein, at 5,568,806 to 5,569,162
_5019
Pf1N1B4_5020 - FIG00954920: hypothetical protein, at 5,569,375 to 5,569,677
_5020
Pf1N1B4_5021 - FIG00958580: hypothetical protein, at 5,569,700 to 5,569,876
_5021
Position (kb) 5568
5569 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5567.533 kb on - strand at 5567.552 kb on + strand at 5567.553 kb on - strand at 5567.581 kb on + strand at 5567.583 kb on + strand at 5567.583 kb on + strand at 5567.583 kb on + strand at 5567.584 kb on - strand at 5567.584 kb on - strand at 5567.584 kb on - strand at 5567.651 kb on - strand at 5567.712 kb on + strand at 5567.712 kb on + strand at 5567.712 kb on + strand at 5567.713 kb on - strand at 5567.713 kb on - strand at 5567.713 kb on - strand at 5567.713 kb on - strand at 5567.955 kb on + strand at 5568.110 kb on + strand, within Pf1N1B4_5017 at 5568.110 kb on + strand, within Pf1N1B4_5017 at 5568.238 kb on + strand, within Pf1N1B4_5017 at 5568.291 kb on - strand, within Pf1N1B4_5017 at 5568.323 kb on + strand at 5568.323 kb on + strand at 5568.323 kb on + strand at 5568.323 kb on + strand at 5568.324 kb on - strand at 5568.342 kb on - strand at 5568.361 kb on - strand at 5568.504 kb on + strand at 5568.504 kb on + strand at 5568.504 kb on + strand at 5568.505 kb on - strand at 5568.505 kb on - strand at 5568.508 kb on + strand at 5568.509 kb on - strand at 5568.509 kb on - strand at 5568.509 kb on - strand at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.692 kb on - strand at 5568.692 kb on - strand, within Pf1N1B4_5018 at 5568.804 kb on + strand at 5568.805 kb on - strand at 5568.805 kb on - strand at 5568.839 kb on + strand at 5568.840 kb on - strand at 5568.840 kb on - strand at 5568.840 kb on - strand at 5568.840 kb on - strand at 5568.840 kb on - strand at 5568.924 kb on - strand, within Pf1N1B4_5019 at 5569.064 kb on + strand, within Pf1N1B4_5019 at 5569.229 kb on - strand at 5569.237 kb on + strand at 5569.237 kb on + strand at 5569.237 kb on + strand at 5569.250 kb on - strand at 5569.699 kb on + strand at 5569.699 kb on + strand at 5569.700 kb on - strand at 5569.700 kb on - strand at 5569.725 kb on - strand at 5569.725 kb on - strand, within Pf1N1B4_5021
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 5,567,533 - -0.7 5,567,552 + -1.2 5,567,553 - -0.4 5,567,581 + -0.1 5,567,583 + +0.8 5,567,583 + +0.2 5,567,583 + -0.1 5,567,584 - +0.0 5,567,584 - +0.0 5,567,584 - -1.6 5,567,651 - -1.2 5,567,712 + +0.6 5,567,712 + -0.6 5,567,712 + +0.6 5,567,713 - +0.2 5,567,713 - +0.2 5,567,713 - -0.7 5,567,713 - -1.0 5,567,955 + -0.4 5,568,110 + Pf1N1B4_5017 0.23 -0.4 5,568,110 + Pf1N1B4_5017 0.23 -0.1 5,568,238 + Pf1N1B4_5017 0.69 -1.4 5,568,291 - Pf1N1B4_5017 0.88 +0.4 5,568,323 + +0.0 5,568,323 + -0.9 5,568,323 + -2.3 5,568,323 + +0.3 5,568,324 - +1.0 5,568,342 - +0.8 5,568,361 - +0.5 5,568,504 + +0.4 5,568,504 + -0.7 5,568,504 + -1.0 5,568,505 - +0.3 5,568,505 - -0.6 5,568,508 + +0.7 5,568,509 - -0.3 5,568,509 - +0.7 5,568,509 - +0.3 5,568,691 + Pf1N1B4_5018 0.81 -0.7 5,568,691 + Pf1N1B4_5018 0.81 -0.8 5,568,691 + Pf1N1B4_5018 0.81 -0.6 5,568,691 + Pf1N1B4_5018 0.81 -1.6 5,568,691 + Pf1N1B4_5018 0.81 -0.8 5,568,691 + Pf1N1B4_5018 0.81 -2.3 5,568,692 - +0.4 5,568,692 - Pf1N1B4_5018 0.82 +0.7 5,568,804 + -1.6 5,568,805 - -0.5 5,568,805 - +0.7 5,568,839 + +0.0 5,568,840 - +0.4 5,568,840 - +1.4 5,568,840 - -0.3 5,568,840 - -2.0 5,568,840 - +0.4 5,568,924 - Pf1N1B4_5019 0.33 -1.3 5,569,064 + Pf1N1B4_5019 0.72 +1.3 5,569,229 - +0.0 5,569,237 + -0.6 5,569,237 + +2.1 5,569,237 + +0.0 5,569,250 - +0.0 5,569,699 + +0.2 5,569,699 + +0.5 5,569,700 - -0.6 5,569,700 - -0.6 5,569,725 - +0.6 5,569,725 - Pf1N1B4_5021 0.14 +0.7
Or see this region's nucleotide sequence