Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4149 and Pf1N1B4_4150 are separated by 257 nucleotides
Pf1N1B4_4149 - Probable lipoprotein signal peptide, at 4,594,744 to 4,595,796
_4149
Pf1N1B4_4150 - Transcriptional regulator, AraC family, at 4,596,054 to 4,596,971
_4150
Position (kb)
4594
4595
4596 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4593.941 kb on + strand at 4593.972 kb on + strand at 4593.972 kb on + strand at 4594.196 kb on - strand at 4594.196 kb on - strand at 4594.232 kb on - strand at 4594.341 kb on + strand at 4594.342 kb on - strand at 4594.342 kb on - strand at 4594.342 kb on - strand at 4594.347 kb on + strand at 4594.347 kb on + strand at 4594.348 kb on - strand at 4594.421 kb on - strand at 4594.487 kb on + strand at 4594.499 kb on - strand at 4594.597 kb on - strand at 4594.597 kb on - strand at 4594.597 kb on - strand at 4594.597 kb on - strand at 4594.597 kb on - strand at 4594.665 kb on - strand at 4594.680 kb on + strand at 4594.680 kb on + strand at 4594.680 kb on + strand at 4594.680 kb on + strand at 4594.680 kb on + strand at 4594.680 kb on + strand at 4594.681 kb on - strand at 4594.681 kb on - strand at 4594.681 kb on - strand at 4594.681 kb on - strand at 4594.722 kb on + strand at 4594.723 kb on - strand at 4594.887 kb on + strand, within Pf1N1B4_4149 at 4594.887 kb on + strand, within Pf1N1B4_4149 at 4595.062 kb on + strand, within Pf1N1B4_4149 at 4595.062 kb on + strand, within Pf1N1B4_4149 at 4595.062 kb on + strand, within Pf1N1B4_4149 at 4595.063 kb on - strand, within Pf1N1B4_4149 at 4595.132 kb on + strand, within Pf1N1B4_4149 at 4595.342 kb on - strand, within Pf1N1B4_4149 at 4595.365 kb on - strand at 4595.375 kb on + strand at 4595.495 kb on + strand at 4595.495 kb on + strand, within Pf1N1B4_4149 at 4595.496 kb on - strand, within Pf1N1B4_4149 at 4595.496 kb on - strand, within Pf1N1B4_4149 at 4595.496 kb on - strand, within Pf1N1B4_4149 at 4595.723 kb on + strand at 4595.740 kb on - strand at 4595.740 kb on - strand at 4595.795 kb on - strand at 4595.795 kb on - strand at 4595.795 kb on - strand at 4595.872 kb on + strand at 4595.872 kb on + strand at 4595.873 kb on - strand at 4595.873 kb on - strand at 4595.873 kb on - strand at 4595.876 kb on - strand at 4595.893 kb on + strand at 4595.894 kb on - strand at 4595.894 kb on - strand at 4595.945 kb on + strand at 4595.945 kb on + strand at 4595.977 kb on + strand at 4595.978 kb on - strand at 4595.978 kb on - strand at 4595.992 kb on + strand at 4595.993 kb on - strand at 4595.996 kb on + strand at 4595.996 kb on + strand at 4595.996 kb on + strand at 4595.997 kb on - strand at 4595.997 kb on - strand at 4596.001 kb on + strand at 4596.007 kb on + strand at 4596.008 kb on - strand at 4596.010 kb on + strand at 4596.019 kb on - strand at 4596.019 kb on - strand at 4596.029 kb on - strand at 4596.406 kb on + strand, within Pf1N1B4_4150 at 4596.445 kb on + strand, within Pf1N1B4_4150 at 4596.445 kb on + strand, within Pf1N1B4_4150 at 4596.446 kb on - strand, within Pf1N1B4_4150 at 4596.446 kb on - strand, within Pf1N1B4_4150 at 4596.446 kb on - strand, within Pf1N1B4_4150 at 4596.655 kb on + strand, within Pf1N1B4_4150 at 4596.721 kb on + strand, within Pf1N1B4_4150 at 4596.721 kb on + strand, within Pf1N1B4_4150 at 4596.722 kb on - strand, within Pf1N1B4_4150
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 4,593,941 + -0.4 4,593,972 + -2.4 4,593,972 + -0.2 4,594,196 - -0.2 4,594,196 - -1.1 4,594,232 - +0.8 4,594,341 + +0.8 4,594,342 - +1.0 4,594,342 - -0.3 4,594,342 - +3.0 4,594,347 + -1.8 4,594,347 + -0.8 4,594,348 - +1.8 4,594,421 - -1.1 4,594,487 + +1.4 4,594,499 - +0.1 4,594,597 - +0.8 4,594,597 - +1.8 4,594,597 - +1.0 4,594,597 - +1.2 4,594,597 - +1.0 4,594,665 - +0.4 4,594,680 + -1.0 4,594,680 + +0.9 4,594,680 + +0.2 4,594,680 + -0.3 4,594,680 + -0.8 4,594,680 + -1.6 4,594,681 - -0.6 4,594,681 - -0.5 4,594,681 - +0.7 4,594,681 - +0.1 4,594,722 + +0.8 4,594,723 - +1.2 4,594,887 + Pf1N1B4_4149 0.14 -0.4 4,594,887 + Pf1N1B4_4149 0.14 +0.2 4,595,062 + Pf1N1B4_4149 0.30 +2.0 4,595,062 + Pf1N1B4_4149 0.30 -1.4 4,595,062 + Pf1N1B4_4149 0.30 +0.2 4,595,063 - Pf1N1B4_4149 0.30 -1.4 4,595,132 + Pf1N1B4_4149 0.37 -1.9 4,595,342 - Pf1N1B4_4149 0.57 -1.0 4,595,365 - -0.8 4,595,375 + -1.4 4,595,495 + +0.2 4,595,495 + Pf1N1B4_4149 0.71 -1.0 4,595,496 - Pf1N1B4_4149 0.71 +0.2 4,595,496 - Pf1N1B4_4149 0.71 -1.1 4,595,496 - Pf1N1B4_4149 0.71 -1.0 4,595,723 + -0.4 4,595,740 - -0.0 4,595,740 - -1.1 4,595,795 - -0.3 4,595,795 - +0.2 4,595,795 - -0.5 4,595,872 + +0.2 4,595,872 + -0.4 4,595,873 - -1.4 4,595,873 - +0.0 4,595,873 - +0.0 4,595,876 - +0.5 4,595,893 + -0.2 4,595,894 - -2.4 4,595,894 - -2.6 4,595,945 + +1.7 4,595,945 + -1.8 4,595,977 + +0.6 4,595,978 - -1.3 4,595,978 - +0.2 4,595,992 + -1.9 4,595,993 - +1.2 4,595,996 + -1.6 4,595,996 + -0.8 4,595,996 + -0.1 4,595,997 - +0.9 4,595,997 - +0.1 4,596,001 + -2.1 4,596,007 + +2.2 4,596,008 - +0.2 4,596,010 + -0.3 4,596,019 - +1.1 4,596,019 - -2.4 4,596,029 - -0.9 4,596,406 + Pf1N1B4_4150 0.38 -0.6 4,596,445 + Pf1N1B4_4150 0.43 -1.2 4,596,445 + Pf1N1B4_4150 0.43 +0.2 4,596,446 - Pf1N1B4_4150 0.43 +0.5 4,596,446 - Pf1N1B4_4150 0.43 -1.1 4,596,446 - Pf1N1B4_4150 0.43 +0.1 4,596,655 + Pf1N1B4_4150 0.65 +0.4 4,596,721 + Pf1N1B4_4150 0.73 +0.0 4,596,721 + Pf1N1B4_4150 0.73 -0.6 4,596,722 - Pf1N1B4_4150 0.73 -0.1
Or see this region's nucleotide sequence