Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4060 and Pf1N1B4_4061 are separated by 68 nucleotides Pf1N1B4_4061 and Pf1N1B4_4062 are separated by 172 nucleotides Pf1N1B4_4062 and Pf1N1B4_4063 overlap by 1 nucleotides Pf1N1B4_4063 and Pf1N1B4_4064 are separated by 15 nucleotides
Pf1N1B4_4060 - Cell division protein FtsK, at 4,513,027 to 4,515,432
_4060
Pf1N1B4_4061 - Outer membrane lipoprotein carrier protein LolA, at 4,515,501 to 4,516,124
_4061
Pf1N1B4_4062 - FIG065221: Holliday junction DNA helicase, at 4,516,297 to 4,517,619
_4062
Pf1N1B4_4063 - CrcB protein, at 4,517,619 to 4,517,993
_4063
Pf1N1B4_4064 - Seryl-tRNA synthetase (EC 6.1.1.11), at 4,518,009 to 4,519,289
_4064
Position (kb)
4516
4517
4518 Strain fitness (log2 ratio)
-2
-1
0
1 at 4515.488 kb on + strand at 4516.123 kb on - strand at 4516.136 kb on + strand at 4516.136 kb on + strand at 4516.136 kb on + strand at 4516.137 kb on - strand at 4516.137 kb on - strand at 4516.137 kb on - strand at 4516.175 kb on + strand at 4516.175 kb on + strand at 4516.175 kb on + strand at 4516.176 kb on - strand at 4516.288 kb on + strand at 4516.288 kb on + strand at 4516.288 kb on + strand at 4516.288 kb on + strand at 4516.288 kb on + strand at 4516.289 kb on - strand at 4516.289 kb on - strand at 4516.289 kb on - strand at 4516.293 kb on - strand at 4516.473 kb on + strand, within Pf1N1B4_4062 at 4516.601 kb on - strand, within Pf1N1B4_4062 at 4516.768 kb on + strand, within Pf1N1B4_4062 at 4516.769 kb on - strand, within Pf1N1B4_4062 at 4517.041 kb on + strand, within Pf1N1B4_4062 at 4517.042 kb on - strand, within Pf1N1B4_4062 at 4517.042 kb on - strand, within Pf1N1B4_4062 at 4517.065 kb on - strand, within Pf1N1B4_4062 at 4517.143 kb on + strand, within Pf1N1B4_4062 at 4517.143 kb on + strand, within Pf1N1B4_4062 at 4517.144 kb on - strand, within Pf1N1B4_4062 at 4517.287 kb on - strand, within Pf1N1B4_4062 at 4517.290 kb on + strand, within Pf1N1B4_4062 at 4517.291 kb on - strand, within Pf1N1B4_4062 at 4517.291 kb on - strand, within Pf1N1B4_4062 at 4517.323 kb on + strand, within Pf1N1B4_4062 at 4517.324 kb on - strand, within Pf1N1B4_4062 at 4517.324 kb on - strand, within Pf1N1B4_4062 at 4517.488 kb on + strand at 4517.489 kb on - strand at 4517.489 kb on - strand at 4517.489 kb on - strand at 4517.594 kb on + strand at 4517.595 kb on - strand at 4517.721 kb on + strand, within Pf1N1B4_4063 at 4517.721 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.941 kb on + strand, within Pf1N1B4_4063
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 4,515,488 + -0.4 4,516,123 - -0.2 4,516,136 + +0.2 4,516,136 + -0.8 4,516,136 + -1.1 4,516,137 - -0.8 4,516,137 - -0.9 4,516,137 - -2.7 4,516,175 + +0.6 4,516,175 + +0.1 4,516,175 + +1.6 4,516,176 - +0.2 4,516,288 + +0.2 4,516,288 + -1.1 4,516,288 + -2.1 4,516,288 + -1.8 4,516,288 + -2.0 4,516,289 - -1.6 4,516,289 - -0.8 4,516,289 - +0.2 4,516,293 - +0.2 4,516,473 + Pf1N1B4_4062 0.13 -0.9 4,516,601 - Pf1N1B4_4062 0.23 -1.4 4,516,768 + Pf1N1B4_4062 0.36 -0.4 4,516,769 - Pf1N1B4_4062 0.36 -1.1 4,517,041 + Pf1N1B4_4062 0.56 -1.4 4,517,042 - Pf1N1B4_4062 0.56 -2.1 4,517,042 - Pf1N1B4_4062 0.56 -0.4 4,517,065 - Pf1N1B4_4062 0.58 -2.5 4,517,143 + Pf1N1B4_4062 0.64 -0.8 4,517,143 + Pf1N1B4_4062 0.64 -0.8 4,517,144 - Pf1N1B4_4062 0.64 -0.3 4,517,287 - Pf1N1B4_4062 0.75 -2.6 4,517,290 + Pf1N1B4_4062 0.75 -2.4 4,517,291 - Pf1N1B4_4062 0.75 -0.8 4,517,291 - Pf1N1B4_4062 0.75 +0.2 4,517,323 + Pf1N1B4_4062 0.78 -0.8 4,517,324 - Pf1N1B4_4062 0.78 -1.1 4,517,324 - Pf1N1B4_4062 0.78 +0.2 4,517,488 + -1.1 4,517,489 - -1.0 4,517,489 - +1.2 4,517,489 - -0.6 4,517,594 + -2.5 4,517,595 - +0.7 4,517,721 + Pf1N1B4_4063 0.27 -0.0 4,517,721 + Pf1N1B4_4063 0.27 -1.6 4,517,773 + Pf1N1B4_4063 0.41 +1.6 4,517,773 + Pf1N1B4_4063 0.41 -1.2 4,517,773 + Pf1N1B4_4063 0.41 +1.6 4,517,773 + Pf1N1B4_4063 0.41 +0.2 4,517,773 + Pf1N1B4_4063 0.41 -1.9 4,517,773 + Pf1N1B4_4063 0.41 -2.1 4,517,941 + Pf1N1B4_4063 0.86 +0.9
Or see this region's nucleotide sequence