Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4024

Experiment: methylglyoxal 0.004 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4022 and Pf1N1B4_4023 are separated by 79 nucleotidesPf1N1B4_4023 and Pf1N1B4_4024 are separated by 477 nucleotidesPf1N1B4_4024 and Pf1N1B4_4025 are separated by 207 nucleotidesPf1N1B4_4025 and Pf1N1B4_4026 overlap by 4 nucleotides Pf1N1B4_4022 - hypothetical protein, at 4,474,955 to 4,475,155 _4022 Pf1N1B4_4023 - hypothetical protein, at 4,475,235 to 4,475,567 _4023 Pf1N1B4_4024 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 4,476,045 to 4,476,425 _4024 Pf1N1B4_4025 - Outer membrane porin, OprD family, at 4,476,633 to 4,477,394 _4025 Pf1N1B4_4026 - Outer membrane porin, OprD family, at 4,477,391 to 4,477,894 _4026 Position (kb) 4476 4477Strain fitness (log2 ratio) -2 -1 0 1 2at 4475.143 kb on + strandat 4475.153 kb on + strandat 4475.153 kb on + strandat 4475.153 kb on + strandat 4475.153 kb on + strandat 4475.153 kb on + strandat 4475.154 kb on - strandat 4475.234 kb on + strandat 4475.235 kb on - strandat 4475.235 kb on - strandat 4475.340 kb on - strand, within Pf1N1B4_4023at 4475.361 kb on + strand, within Pf1N1B4_4023at 4475.480 kb on + strand, within Pf1N1B4_4023at 4475.493 kb on + strand, within Pf1N1B4_4023at 4475.493 kb on + strand, within Pf1N1B4_4023at 4475.493 kb on + strand, within Pf1N1B4_4023at 4475.493 kb on + strand, within Pf1N1B4_4023at 4475.516 kb on + strand, within Pf1N1B4_4023at 4475.516 kb on + strand, within Pf1N1B4_4023at 4475.516 kb on + strand, within Pf1N1B4_4023at 4475.530 kb on - strand, within Pf1N1B4_4023at 4475.530 kb on - strand, within Pf1N1B4_4023at 4475.693 kb on + strandat 4475.704 kb on + strandat 4475.704 kb on + strandat 4475.705 kb on - strandat 4475.741 kb on + strandat 4475.741 kb on + strandat 4475.742 kb on - strandat 4475.875 kb on + strandat 4475.875 kb on + strandat 4475.875 kb on + strandat 4475.875 kb on + strandat 4475.875 kb on + strandat 4475.875 kb on + strandat 4475.876 kb on - strandat 4475.876 kb on - strandat 4475.876 kb on - strandat 4475.876 kb on - strandat 4475.901 kb on - strandat 4475.978 kb on - strandat 4475.978 kb on - strandat 4476.024 kb on + strandat 4476.165 kb on + strand, within Pf1N1B4_4024at 4476.166 kb on - strand, within Pf1N1B4_4024at 4476.168 kb on + strand, within Pf1N1B4_4024at 4476.168 kb on + strand, within Pf1N1B4_4024at 4476.168 kb on + strand, within Pf1N1B4_4024at 4476.169 kb on - strand, within Pf1N1B4_4024at 4476.169 kb on - strandat 4476.423 kb on + strandat 4476.423 kb on + strandat 4476.423 kb on + strandat 4476.424 kb on - strandat 4476.543 kb on + strandat 4476.544 kb on - strandat 4476.584 kb on - strandat 4476.584 kb on - strandat 4476.606 kb on - strandat 4476.653 kb on - strandat 4476.669 kb on + strandat 4476.763 kb on + strandat 4476.764 kb on - strand, within Pf1N1B4_4025at 4476.764 kb on - strand, within Pf1N1B4_4025at 4476.764 kb on - strand, within Pf1N1B4_4025at 4476.764 kb on - strand, within Pf1N1B4_4025at 4476.764 kb on - strand, within Pf1N1B4_4025at 4476.764 kb on - strand, within Pf1N1B4_4025at 4476.835 kb on + strand, within Pf1N1B4_4025at 4476.835 kb on + strand, within Pf1N1B4_4025at 4476.835 kb on + strandat 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strandat 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.857 kb on - strand, within Pf1N1B4_4025at 4476.913 kb on + strand, within Pf1N1B4_4025at 4476.913 kb on + strand, within Pf1N1B4_4025at 4476.913 kb on + strand, within Pf1N1B4_4025at 4476.914 kb on - strand, within Pf1N1B4_4025at 4476.979 kb on + strand, within Pf1N1B4_4025at 4476.980 kb on - strand, within Pf1N1B4_4025at 4477.162 kb on + strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.166 kb on - strand, within Pf1N1B4_4025at 4477.166 kb on - strand, within Pf1N1B4_4025at 4477.166 kb on - strand, within Pf1N1B4_4025at 4477.166 kb on - strand, within Pf1N1B4_4025at 4477.228 kb on + strand, within Pf1N1B4_4025at 4477.228 kb on + strand, within Pf1N1B4_4025at 4477.229 kb on - strand, within Pf1N1B4_4025at 4477.229 kb on - strand, within Pf1N1B4_4025at 4477.229 kb on - strand, within Pf1N1B4_4025at 4477.232 kb on - strand, within Pf1N1B4_4025at 4477.232 kb on - strand, within Pf1N1B4_4025at 4477.232 kb on - strand, within Pf1N1B4_4025at 4477.265 kb on - strand, within Pf1N1B4_4025at 4477.298 kb on - strand, within Pf1N1B4_4025at 4477.345 kb on + strandat 4477.346 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol%
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4,475,143 + +0.3
4,475,153 + -0.0
4,475,153 + +0.8
4,475,153 + -1.7
4,475,153 + +0.3
4,475,153 + -0.9
4,475,154 - -0.7
4,475,234 + -2.6
4,475,235 - -0.0
4,475,235 - +0.9
4,475,340 - Pf1N1B4_4023 0.32 -0.7
4,475,361 + Pf1N1B4_4023 0.38 +1.1
4,475,480 + Pf1N1B4_4023 0.74 -1.5
4,475,493 + Pf1N1B4_4023 0.77 +2.3
4,475,493 + Pf1N1B4_4023 0.77 +0.6
4,475,493 + Pf1N1B4_4023 0.77 +0.6
4,475,493 + Pf1N1B4_4023 0.77 -0.6
4,475,516 + Pf1N1B4_4023 0.84 +1.1
4,475,516 + Pf1N1B4_4023 0.84 -1.0
4,475,516 + Pf1N1B4_4023 0.84 -0.7
4,475,530 - Pf1N1B4_4023 0.89 -0.7
4,475,530 - Pf1N1B4_4023 0.89 +0.7
4,475,693 + +0.4
4,475,704 + -0.3
4,475,704 + +2.1
4,475,705 - -0.6
4,475,741 + -0.0
4,475,741 + -0.1
4,475,742 - -0.5
4,475,875 + +0.2
4,475,875 + -2.1
4,475,875 + +0.7
4,475,875 + +0.6
4,475,875 + -1.1
4,475,875 + -0.2
4,475,876 - +0.2
4,475,876 - +1.8
4,475,876 - +0.2
4,475,876 - -0.1
4,475,901 - -2.3
4,475,978 - -0.1
4,475,978 - +2.2
4,476,024 + -0.1
4,476,165 + Pf1N1B4_4024 0.31 +0.2
4,476,166 - Pf1N1B4_4024 0.32 -1.1
4,476,168 + Pf1N1B4_4024 0.32 -1.8
4,476,168 + Pf1N1B4_4024 0.32 -0.5
4,476,168 + Pf1N1B4_4024 0.32 -0.8
4,476,169 - Pf1N1B4_4024 0.33 -1.6
4,476,169 - +1.8
4,476,423 + +0.5
4,476,423 + -0.2
4,476,423 + -0.5
4,476,424 - -0.2
4,476,543 + -0.2
4,476,544 - -1.3
4,476,584 - -0.8
4,476,584 - -1.8
4,476,606 - -0.3
4,476,653 - -0.4
4,476,669 + -1.8
4,476,763 + +0.2
4,476,764 - Pf1N1B4_4025 0.17 -0.3
4,476,764 - Pf1N1B4_4025 0.17 -1.9
4,476,764 - Pf1N1B4_4025 0.17 -0.6
4,476,764 - Pf1N1B4_4025 0.17 -1.2
4,476,764 - Pf1N1B4_4025 0.17 +0.9
4,476,764 - Pf1N1B4_4025 0.17 +0.3
4,476,835 + Pf1N1B4_4025 0.27 -0.3
4,476,835 + Pf1N1B4_4025 0.27 -1.0
4,476,835 + -1.3
4,476,836 - Pf1N1B4_4025 0.27 +1.2
4,476,836 - Pf1N1B4_4025 0.27 +0.5
4,476,836 - Pf1N1B4_4025 0.27 +1.1
4,476,836 - Pf1N1B4_4025 0.27 -0.9
4,476,836 - -1.1
4,476,836 - Pf1N1B4_4025 0.27 -0.3
4,476,836 - Pf1N1B4_4025 0.27 +0.5
4,476,836 - Pf1N1B4_4025 0.27 +0.3
4,476,836 - Pf1N1B4_4025 0.27 +0.2
4,476,836 - Pf1N1B4_4025 0.27 +0.5
4,476,836 - Pf1N1B4_4025 0.27 +0.1
4,476,836 - Pf1N1B4_4025 0.27 -0.0
4,476,857 - Pf1N1B4_4025 0.29 +1.2
4,476,913 + Pf1N1B4_4025 0.37 +1.7
4,476,913 + Pf1N1B4_4025 0.37 -0.1
4,476,913 + Pf1N1B4_4025 0.37 -0.2
4,476,914 - Pf1N1B4_4025 0.37 -0.6
4,476,979 + Pf1N1B4_4025 0.45 -0.6
4,476,980 - Pf1N1B4_4025 0.46 +0.4
4,477,162 + Pf1N1B4_4025 0.69 +0.2
4,477,163 - Pf1N1B4_4025 0.70 +0.4
4,477,163 - Pf1N1B4_4025 0.70 -0.2
4,477,163 - Pf1N1B4_4025 0.70 -0.0
4,477,163 - Pf1N1B4_4025 0.70 -0.9
4,477,163 - Pf1N1B4_4025 0.70 +1.1
4,477,163 - Pf1N1B4_4025 0.70 +0.8
4,477,163 - Pf1N1B4_4025 0.70 +0.5
4,477,163 - Pf1N1B4_4025 0.70 +1.1
4,477,163 - Pf1N1B4_4025 0.70 +0.2
4,477,163 - Pf1N1B4_4025 0.70 +0.2
4,477,163 - Pf1N1B4_4025 0.70 +1.5
4,477,166 - Pf1N1B4_4025 0.70 -0.2
4,477,166 - Pf1N1B4_4025 0.70 -1.8
4,477,166 - Pf1N1B4_4025 0.70 -0.7
4,477,166 - Pf1N1B4_4025 0.70 -0.0
4,477,228 + Pf1N1B4_4025 0.78 -0.9
4,477,228 + Pf1N1B4_4025 0.78 -0.1
4,477,229 - Pf1N1B4_4025 0.78 +0.6
4,477,229 - Pf1N1B4_4025 0.78 -0.3
4,477,229 - Pf1N1B4_4025 0.78 +2.2
4,477,232 - Pf1N1B4_4025 0.79 +0.5
4,477,232 - Pf1N1B4_4025 0.79 -1.6
4,477,232 - Pf1N1B4_4025 0.79 -0.2
4,477,265 - Pf1N1B4_4025 0.83 +0.0
4,477,298 - Pf1N1B4_4025 0.87 +0.0
4,477,345 + -1.3
4,477,346 - -1.1

Or see this region's nucleotide sequence