Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3955 and Pf1N1B4_3956 are separated by 419 nucleotides Pf1N1B4_3956 and Pf1N1B4_3957 are separated by 170 nucleotides Pf1N1B4_3957 and Pf1N1B4_3958 are separated by 190 nucleotides
Pf1N1B4_3955 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 4,392,876 to 4,394,915
_3955
Pf1N1B4_3956 - 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7), at 4,395,335 to 4,396,030
_3956
Pf1N1B4_3957 - cytochrome b(561), at 4,396,201 to 4,396,749
_3957
Pf1N1B4_3958 - Phosphonoacetaldehyde hydrolase (EC 3.11.1.1), at 4,396,940 to 4,397,767
_3958
Position (kb)
4395
4396
4397 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4394.642 kb on + strand, within Pf1N1B4_3955 at 4394.913 kb on + strand at 4394.913 kb on + strand at 4394.913 kb on + strand at 4394.914 kb on - strand at 4394.916 kb on + strand at 4394.916 kb on + strand at 4394.917 kb on - strand at 4394.917 kb on - strand at 4394.922 kb on + strand at 4394.922 kb on + strand at 4394.922 kb on + strand at 4394.922 kb on + strand at 4394.922 kb on + strand at 4394.922 kb on + strand at 4394.923 kb on - strand at 4394.987 kb on + strand at 4394.987 kb on + strand at 4394.988 kb on - strand at 4395.071 kb on + strand at 4395.071 kb on + strand at 4395.072 kb on - strand at 4395.523 kb on - strand, within Pf1N1B4_3956 at 4395.523 kb on - strand, within Pf1N1B4_3956 at 4395.627 kb on - strand, within Pf1N1B4_3956 at 4395.707 kb on + strand, within Pf1N1B4_3956 at 4395.708 kb on - strand, within Pf1N1B4_3956 at 4395.759 kb on + strand at 4395.759 kb on + strand, within Pf1N1B4_3956 at 4395.759 kb on + strand, within Pf1N1B4_3956 at 4395.759 kb on + strand, within Pf1N1B4_3956 at 4395.759 kb on + strand, within Pf1N1B4_3956 at 4395.759 kb on + strand, within Pf1N1B4_3956 at 4395.759 kb on + strand, within Pf1N1B4_3956 at 4395.760 kb on - strand, within Pf1N1B4_3956 at 4395.760 kb on - strand, within Pf1N1B4_3956 at 4395.760 kb on - strand at 4395.794 kb on + strand, within Pf1N1B4_3956 at 4395.902 kb on + strand, within Pf1N1B4_3956 at 4396.043 kb on + strand at 4396.043 kb on + strand at 4396.250 kb on + strand at 4396.251 kb on - strand at 4396.251 kb on - strand at 4396.251 kb on - strand at 4396.251 kb on - strand at 4396.251 kb on - strand at 4396.251 kb on - strand at 4396.260 kb on - strand at 4396.260 kb on - strand, within Pf1N1B4_3957 at 4396.260 kb on - strand, within Pf1N1B4_3957 at 4396.385 kb on + strand, within Pf1N1B4_3957 at 4396.386 kb on - strand, within Pf1N1B4_3957 at 4396.386 kb on - strand, within Pf1N1B4_3957 at 4396.455 kb on + strand, within Pf1N1B4_3957 at 4396.456 kb on - strand, within Pf1N1B4_3957 at 4396.456 kb on - strand, within Pf1N1B4_3957 at 4396.458 kb on - strand, within Pf1N1B4_3957 at 4396.458 kb on - strand at 4396.664 kb on + strand, within Pf1N1B4_3957 at 4396.665 kb on - strand, within Pf1N1B4_3957 at 4396.665 kb on - strand, within Pf1N1B4_3957 at 4396.682 kb on - strand, within Pf1N1B4_3957 at 4396.711 kb on + strand at 4396.711 kb on + strand at 4396.722 kb on - strand at 4396.749 kb on + strand at 4396.749 kb on + strand at 4396.813 kb on + strand at 4396.813 kb on + strand at 4396.813 kb on + strand at 4396.813 kb on + strand at 4396.813 kb on + strand at 4396.872 kb on - strand at 4396.872 kb on - strand at 4397.028 kb on + strand at 4397.028 kb on + strand, within Pf1N1B4_3958 at 4397.028 kb on + strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 4,394,642 + Pf1N1B4_3955 0.87 -1.3 4,394,913 + +0.8 4,394,913 + -1.3 4,394,913 + +0.1 4,394,914 - +0.3 4,394,916 + -0.7 4,394,916 + -0.7 4,394,917 - +0.7 4,394,917 - +1.3 4,394,922 + +1.3 4,394,922 + -0.9 4,394,922 + +2.1 4,394,922 + -0.3 4,394,922 + +0.7 4,394,922 + -0.3 4,394,923 - +0.6 4,394,987 + -2.3 4,394,987 + -1.7 4,394,988 - -0.0 4,395,071 + -1.3 4,395,071 + +0.2 4,395,072 - +0.7 4,395,523 - Pf1N1B4_3956 0.27 -1.9 4,395,523 - Pf1N1B4_3956 0.27 -0.9 4,395,627 - Pf1N1B4_3956 0.42 -1.3 4,395,707 + Pf1N1B4_3956 0.53 -1.1 4,395,708 - Pf1N1B4_3956 0.54 -1.3 4,395,759 + -0.7 4,395,759 + Pf1N1B4_3956 0.61 +0.3 4,395,759 + Pf1N1B4_3956 0.61 -0.5 4,395,759 + Pf1N1B4_3956 0.61 -1.3 4,395,759 + Pf1N1B4_3956 0.61 -0.2 4,395,759 + Pf1N1B4_3956 0.61 +0.9 4,395,759 + Pf1N1B4_3956 0.61 -0.1 4,395,760 - Pf1N1B4_3956 0.61 -1.7 4,395,760 - Pf1N1B4_3956 0.61 -1.3 4,395,760 - -0.1 4,395,794 + Pf1N1B4_3956 0.66 -0.5 4,395,902 + Pf1N1B4_3956 0.81 -0.5 4,396,043 + +0.0 4,396,043 + -2.3 4,396,250 + +0.7 4,396,251 - -0.1 4,396,251 - +0.3 4,396,251 - -1.9 4,396,251 - +0.5 4,396,251 - -0.6 4,396,251 - -0.7 4,396,260 - -0.3 4,396,260 - Pf1N1B4_3957 0.11 +2.0 4,396,260 - Pf1N1B4_3957 0.11 +0.1 4,396,385 + Pf1N1B4_3957 0.34 +0.2 4,396,386 - Pf1N1B4_3957 0.34 -2.0 4,396,386 - Pf1N1B4_3957 0.34 -0.1 4,396,455 + Pf1N1B4_3957 0.46 +2.7 4,396,456 - Pf1N1B4_3957 0.46 -0.6 4,396,456 - Pf1N1B4_3957 0.46 +1.3 4,396,458 - Pf1N1B4_3957 0.47 +0.9 4,396,458 - +1.3 4,396,664 + Pf1N1B4_3957 0.84 -0.5 4,396,665 - Pf1N1B4_3957 0.85 -0.7 4,396,665 - Pf1N1B4_3957 0.85 -0.5 4,396,682 - Pf1N1B4_3957 0.88 -2.0 4,396,711 + -0.9 4,396,711 + +1.2 4,396,722 - +0.5 4,396,749 + -0.1 4,396,749 + +0.1 4,396,813 + +0.2 4,396,813 + -0.9 4,396,813 + -0.3 4,396,813 + -0.3 4,396,813 + -1.3 4,396,872 - +0.7 4,396,872 - -1.1 4,397,028 + +1.5 4,397,028 + Pf1N1B4_3958 0.11 +0.0 4,397,028 + Pf1N1B4_3958 0.11 +1.3 4,397,029 - Pf1N1B4_3958 0.11 +0.3 4,397,029 - Pf1N1B4_3958 0.11 -0.7 4,397,029 - Pf1N1B4_3958 0.11 -0.7 4,397,029 - Pf1N1B4_3958 0.11 -0.0 4,397,029 - Pf1N1B4_3958 0.11 -0.4 4,397,029 - Pf1N1B4_3958 0.11 +0.1 4,397,029 - Pf1N1B4_3958 0.11 +0.3 4,397,029 - Pf1N1B4_3958 0.11 +0.4 4,397,029 - Pf1N1B4_3958 0.11 -0.7
Or see this region's nucleotide sequence