Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3904 and Pf1N1B4_3905 are separated by 98 nucleotides Pf1N1B4_3905 and Pf1N1B4_3906 are separated by 114 nucleotides Pf1N1B4_3906 and Pf1N1B4_3907 are separated by 138 nucleotides Pf1N1B4_3907 and Pf1N1B4_3908 are separated by 141 nucleotides
Pf1N1B4_3904 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 4,335,300 to 4,336,451
_3904
Pf1N1B4_3905 - FIG00953423: hypothetical protein, at 4,336,550 to 4,336,786
_3905
Pf1N1B4_3906 - DNA topoisomerase I (EC 5.99.1.2), at 4,336,901 to 4,339,528
_3906
Pf1N1B4_3907 - FIG00953287: hypothetical protein, at 4,339,667 to 4,340,191
_3907
Pf1N1B4_3908 - FIG00954079: hypothetical protein, at 4,340,333 to 4,340,566
_3908
Position (kb)
4336
4337
4338
4339
4340 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4336.519 kb on - strand at 4336.581 kb on - strand, within Pf1N1B4_3905 at 4336.581 kb on - strand, within Pf1N1B4_3905 at 4336.581 kb on - strand, within Pf1N1B4_3905 at 4336.581 kb on - strand, within Pf1N1B4_3905 at 4336.581 kb on - strand, within Pf1N1B4_3905 at 4336.603 kb on - strand, within Pf1N1B4_3905 at 4336.603 kb on - strand, within Pf1N1B4_3905 at 4336.603 kb on - strand, within Pf1N1B4_3905 at 4336.649 kb on + strand, within Pf1N1B4_3905 at 4336.650 kb on - strand, within Pf1N1B4_3905 at 4336.650 kb on - strand at 4336.650 kb on - strand, within Pf1N1B4_3905 at 4336.835 kb on + strand at 4336.835 kb on + strand at 4336.835 kb on + strand at 4336.835 kb on + strand at 4336.837 kb on + strand at 4336.837 kb on + strand at 4336.837 kb on + strand at 4336.837 kb on + strand at 4336.837 kb on + strand at 4336.837 kb on + strand at 4336.837 kb on + strand at 4336.839 kb on + strand at 4336.877 kb on + strand at 4336.877 kb on + strand at 4336.877 kb on + strand at 4336.877 kb on + strand at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.221 kb on - strand, within Pf1N1B4_3906 at 4339.427 kb on + strand at 4339.428 kb on - strand at 4339.526 kb on + strand at 4339.526 kb on + strand at 4339.537 kb on - strand at 4339.644 kb on - strand at 4339.644 kb on - strand at 4339.644 kb on - strand at 4339.682 kb on + strand at 4339.682 kb on + strand at 4339.683 kb on - strand at 4339.683 kb on - strand at 4339.683 kb on - strand at 4339.683 kb on - strand at 4339.706 kb on + strand at 4339.707 kb on - strand at 4339.707 kb on - strand at 4339.707 kb on - strand at 4339.707 kb on - strand at 4339.707 kb on - strand at 4339.857 kb on - strand, within Pf1N1B4_3907 at 4339.928 kb on - strand, within Pf1N1B4_3907 at 4340.064 kb on + strand, within Pf1N1B4_3907 at 4340.077 kb on + strand, within Pf1N1B4_3907 at 4340.147 kb on - strand at 4340.299 kb on + strand at 4340.299 kb on + strand at 4340.299 kb on + strand at 4340.299 kb on + strand at 4340.300 kb on - strand at 4340.300 kb on - strand at 4340.300 kb on - strand at 4340.301 kb on + strand at 4340.301 kb on + strand at 4340.301 kb on + strand at 4340.301 kb on + strand at 4340.302 kb on - strand at 4340.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 4,336,519 - +0.6 4,336,581 - Pf1N1B4_3905 0.13 -0.0 4,336,581 - Pf1N1B4_3905 0.13 +0.3 4,336,581 - Pf1N1B4_3905 0.13 -0.5 4,336,581 - Pf1N1B4_3905 0.13 -0.2 4,336,581 - Pf1N1B4_3905 0.13 +0.3 4,336,603 - Pf1N1B4_3905 0.22 -1.3 4,336,603 - Pf1N1B4_3905 0.22 -0.2 4,336,603 - Pf1N1B4_3905 0.22 +1.0 4,336,649 + Pf1N1B4_3905 0.42 +2.1 4,336,650 - Pf1N1B4_3905 0.42 +0.8 4,336,650 - +0.3 4,336,650 - Pf1N1B4_3905 0.42 +1.0 4,336,835 + -0.5 4,336,835 + -2.0 4,336,835 + -2.3 4,336,835 + -1.3 4,336,837 + -0.3 4,336,837 + +1.2 4,336,837 + -2.0 4,336,837 + -0.4 4,336,837 + -0.3 4,336,837 + +0.3 4,336,837 + -1.5 4,336,839 + -2.7 4,336,877 + -0.5 4,336,877 + +0.0 4,336,877 + -0.5 4,336,877 + -1.3 4,339,220 + Pf1N1B4_3906 0.88 +0.8 4,339,220 + Pf1N1B4_3906 0.88 -1.7 4,339,220 + Pf1N1B4_3906 0.88 -1.1 4,339,220 + Pf1N1B4_3906 0.88 -0.9 4,339,220 + Pf1N1B4_3906 0.88 -1.8 4,339,220 + Pf1N1B4_3906 0.88 -0.8 4,339,220 + -1.7 4,339,220 + Pf1N1B4_3906 0.88 +0.1 4,339,220 + Pf1N1B4_3906 0.88 -0.4 4,339,221 - Pf1N1B4_3906 0.88 -2.1 4,339,427 + -0.9 4,339,428 - -3.0 4,339,526 + -0.4 4,339,526 + +1.0 4,339,537 - -1.3 4,339,644 - +0.5 4,339,644 - -0.7 4,339,644 - -1.7 4,339,682 + +0.7 4,339,682 + +0.6 4,339,683 - -0.8 4,339,683 - +2.3 4,339,683 - +0.1 4,339,683 - +1.3 4,339,706 + +0.7 4,339,707 - -0.5 4,339,707 - +0.0 4,339,707 - +0.0 4,339,707 - -0.3 4,339,707 - -0.7 4,339,857 - Pf1N1B4_3907 0.36 +1.6 4,339,928 - Pf1N1B4_3907 0.50 -0.3 4,340,064 + Pf1N1B4_3907 0.76 +1.3 4,340,077 + Pf1N1B4_3907 0.78 -0.1 4,340,147 - +1.2 4,340,299 + +0.1 4,340,299 + +0.9 4,340,299 + -2.5 4,340,299 + -0.0 4,340,300 - +1.3 4,340,300 - +0.3 4,340,300 - -0.7 4,340,301 + -0.9 4,340,301 + +0.9 4,340,301 + -0.2 4,340,301 + -0.1 4,340,302 - -2.5 4,340,302 - +0.4
Or see this region's nucleotide sequence