Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3666 and Pf1N1B4_3667 are separated by 176 nucleotides Pf1N1B4_3667 and Pf1N1B4_3668 overlap by 8 nucleotides Pf1N1B4_3668 and Pf1N1B4_3669 are separated by 223 nucleotides Pf1N1B4_3669 and Pf1N1B4_3670 are separated by 28 nucleotides
Pf1N1B4_3666 - Membrane protein, putative, at 4,070,551 to 4,071,465
_3666
Pf1N1B4_3667 - Nucleoside ABC transporter, periplasmic nucleoside-binding protein, at 4,071,642 to 4,072,430
_3667
Pf1N1B4_3668 - Nucleoside ABC transporter, periplasmic nucleoside-binding protein, at 4,072,423 to 4,073,721
_3668
Pf1N1B4_3669 - hypothetical protein, at 4,073,945 to 4,074,313
_3669
Pf1N1B4_3670 - probable glucosyl transferase, at 4,074,342 to 4,075,916
_3670
Position (kb)
4072
4073
4074 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4071.471 kb on + strand at 4071.526 kb on + strand at 4071.526 kb on + strand at 4071.578 kb on + strand at 4071.579 kb on - strand at 4071.579 kb on - strand at 4071.590 kb on - strand at 4071.644 kb on - strand at 4071.662 kb on + strand at 4071.675 kb on - strand at 4071.676 kb on + strand at 4071.676 kb on + strand at 4071.677 kb on - strand at 4071.677 kb on - strand at 4071.677 kb on - strand at 4071.682 kb on + strand at 4071.683 kb on - strand at 4071.683 kb on - strand at 4071.683 kb on - strand at 4071.697 kb on + strand at 4071.742 kb on + strand, within Pf1N1B4_3667 at 4071.742 kb on + strand, within Pf1N1B4_3667 at 4071.835 kb on + strand, within Pf1N1B4_3667 at 4071.835 kb on + strand, within Pf1N1B4_3667 at 4071.835 kb on + strand, within Pf1N1B4_3667 at 4071.836 kb on - strand, within Pf1N1B4_3667 at 4071.836 kb on - strand, within Pf1N1B4_3667 at 4071.836 kb on - strand, within Pf1N1B4_3667 at 4071.899 kb on - strand, within Pf1N1B4_3667 at 4071.899 kb on - strand, within Pf1N1B4_3667 at 4071.920 kb on - strand, within Pf1N1B4_3667 at 4071.920 kb on - strand, within Pf1N1B4_3667 at 4071.920 kb on - strand, within Pf1N1B4_3667 at 4072.086 kb on + strand, within Pf1N1B4_3667 at 4072.087 kb on - strand, within Pf1N1B4_3667 at 4072.147 kb on + strand, within Pf1N1B4_3667 at 4072.147 kb on + strand, within Pf1N1B4_3667 at 4072.147 kb on + strand, within Pf1N1B4_3667 at 4072.147 kb on + strand, within Pf1N1B4_3667 at 4072.147 kb on + strand, within Pf1N1B4_3667 at 4072.196 kb on - strand, within Pf1N1B4_3667 at 4072.207 kb on + strand, within Pf1N1B4_3667 at 4072.240 kb on + strand, within Pf1N1B4_3667 at 4072.240 kb on + strand, within Pf1N1B4_3667 at 4072.391 kb on + strand at 4072.420 kb on + strand at 4072.424 kb on + strand at 4072.424 kb on + strand at 4072.424 kb on + strand at 4072.425 kb on - strand at 4072.425 kb on - strand at 4072.610 kb on + strand, within Pf1N1B4_3668 at 4072.610 kb on + strand, within Pf1N1B4_3668 at 4072.610 kb on + strand, within Pf1N1B4_3668 at 4072.611 kb on - strand, within Pf1N1B4_3668 at 4072.625 kb on + strand, within Pf1N1B4_3668 at 4072.626 kb on - strand, within Pf1N1B4_3668 at 4072.626 kb on - strand, within Pf1N1B4_3668 at 4072.709 kb on + strand, within Pf1N1B4_3668 at 4072.709 kb on + strand, within Pf1N1B4_3668 at 4072.709 kb on + strand, within Pf1N1B4_3668 at 4072.753 kb on - strand, within Pf1N1B4_3668 at 4072.919 kb on + strand, within Pf1N1B4_3668 at 4072.947 kb on - strand, within Pf1N1B4_3668 at 4072.947 kb on - strand, within Pf1N1B4_3668 at 4072.947 kb on - strand at 4072.947 kb on - strand, within Pf1N1B4_3668 at 4072.995 kb on - strand, within Pf1N1B4_3668 at 4073.066 kb on + strand, within Pf1N1B4_3668 at 4073.066 kb on + strand, within Pf1N1B4_3668 at 4073.066 kb on + strand, within Pf1N1B4_3668 at 4073.067 kb on - strand, within Pf1N1B4_3668 at 4073.067 kb on - strand, within Pf1N1B4_3668 at 4073.354 kb on + strand, within Pf1N1B4_3668 at 4073.354 kb on + strand, within Pf1N1B4_3668 at 4073.354 kb on + strand, within Pf1N1B4_3668 at 4073.354 kb on + strand, within Pf1N1B4_3668 at 4073.354 kb on + strand, within Pf1N1B4_3668 at 4073.355 kb on - strand, within Pf1N1B4_3668 at 4073.355 kb on - strand, within Pf1N1B4_3668 at 4073.358 kb on - strand, within Pf1N1B4_3668 at 4073.358 kb on - strand, within Pf1N1B4_3668 at 4073.372 kb on + strand, within Pf1N1B4_3668 at 4073.372 kb on + strand, within Pf1N1B4_3668 at 4073.393 kb on + strand, within Pf1N1B4_3668 at 4073.393 kb on + strand, within Pf1N1B4_3668 at 4073.459 kb on + strand, within Pf1N1B4_3668 at 4073.459 kb on + strand, within Pf1N1B4_3668 at 4073.633 kb on + strand at 4073.675 kb on - strand at 4073.725 kb on + strand at 4073.725 kb on + strand at 4073.725 kb on + strand at 4073.725 kb on + strand at 4073.725 kb on + strand at 4073.725 kb on + strand at 4073.726 kb on - strand at 4073.726 kb on - strand at 4073.726 kb on - strand at 4073.726 kb on - strand at 4073.832 kb on + strand at 4073.843 kb on + strand at 4073.856 kb on + strand at 4073.857 kb on - strand at 4073.871 kb on + strand at 4073.871 kb on + strand at 4073.871 kb on + strand at 4073.872 kb on - strand at 4073.931 kb on + strand at 4073.931 kb on + strand at 4074.008 kb on + strand, within Pf1N1B4_3669 at 4074.129 kb on + strand at 4074.239 kb on + strand, within Pf1N1B4_3669 at 4074.304 kb on + strand at 4074.442 kb on + strand at 4074.443 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 4,071,471 + +0.8 4,071,526 + +0.6 4,071,526 + +0.0 4,071,578 + -1.2 4,071,579 - -0.9 4,071,579 - -0.3 4,071,590 - -1.2 4,071,644 - -1.4 4,071,662 + -0.9 4,071,675 - -1.0 4,071,676 + -2.4 4,071,676 + -2.4 4,071,677 - -1.0 4,071,677 - -0.9 4,071,677 - -2.6 4,071,682 + -0.6 4,071,683 - +0.8 4,071,683 - -1.3 4,071,683 - -2.3 4,071,697 + -1.2 4,071,742 + Pf1N1B4_3667 0.13 -1.4 4,071,742 + Pf1N1B4_3667 0.13 -2.3 4,071,835 + Pf1N1B4_3667 0.24 +0.4 4,071,835 + Pf1N1B4_3667 0.24 +0.2 4,071,835 + Pf1N1B4_3667 0.24 -1.5 4,071,836 - Pf1N1B4_3667 0.25 -2.2 4,071,836 - Pf1N1B4_3667 0.25 -2.8 4,071,836 - Pf1N1B4_3667 0.25 -0.6 4,071,899 - Pf1N1B4_3667 0.33 -2.1 4,071,899 - Pf1N1B4_3667 0.33 -0.9 4,071,920 - Pf1N1B4_3667 0.35 +0.0 4,071,920 - Pf1N1B4_3667 0.35 -3.5 4,071,920 - Pf1N1B4_3667 0.35 -0.6 4,072,086 + Pf1N1B4_3667 0.56 -0.2 4,072,087 - Pf1N1B4_3667 0.56 -0.6 4,072,147 + Pf1N1B4_3667 0.64 -0.9 4,072,147 + Pf1N1B4_3667 0.64 -0.6 4,072,147 + Pf1N1B4_3667 0.64 -2.7 4,072,147 + Pf1N1B4_3667 0.64 -2.3 4,072,147 + Pf1N1B4_3667 0.64 -2.2 4,072,196 - Pf1N1B4_3667 0.70 -1.9 4,072,207 + Pf1N1B4_3667 0.72 -1.5 4,072,240 + Pf1N1B4_3667 0.76 +0.3 4,072,240 + Pf1N1B4_3667 0.76 +0.4 4,072,391 + -1.2 4,072,420 + -3.2 4,072,424 + -0.6 4,072,424 + +1.4 4,072,424 + -0.8 4,072,425 - +0.4 4,072,425 - -1.2 4,072,610 + Pf1N1B4_3668 0.14 +0.0 4,072,610 + Pf1N1B4_3668 0.14 +0.9 4,072,610 + Pf1N1B4_3668 0.14 -1.9 4,072,611 - Pf1N1B4_3668 0.14 +0.4 4,072,625 + Pf1N1B4_3668 0.16 -1.2 4,072,626 - Pf1N1B4_3668 0.16 -0.2 4,072,626 - Pf1N1B4_3668 0.16 +0.4 4,072,709 + Pf1N1B4_3668 0.22 -0.9 4,072,709 + Pf1N1B4_3668 0.22 -1.2 4,072,709 + Pf1N1B4_3668 0.22 +2.0 4,072,753 - Pf1N1B4_3668 0.25 -1.8 4,072,919 + Pf1N1B4_3668 0.38 -2.6 4,072,947 - Pf1N1B4_3668 0.40 -2.8 4,072,947 - Pf1N1B4_3668 0.40 -1.9 4,072,947 - +0.4 4,072,947 - Pf1N1B4_3668 0.40 +1.0 4,072,995 - Pf1N1B4_3668 0.44 -2.7 4,073,066 + Pf1N1B4_3668 0.49 -2.0 4,073,066 + Pf1N1B4_3668 0.49 -0.4 4,073,066 + Pf1N1B4_3668 0.49 -1.4 4,073,067 - Pf1N1B4_3668 0.50 -0.9 4,073,067 - Pf1N1B4_3668 0.50 -0.5 4,073,354 + Pf1N1B4_3668 0.72 -1.2 4,073,354 + Pf1N1B4_3668 0.72 -1.7 4,073,354 + Pf1N1B4_3668 0.72 -1.2 4,073,354 + Pf1N1B4_3668 0.72 -1.0 4,073,354 + Pf1N1B4_3668 0.72 -0.2 4,073,355 - Pf1N1B4_3668 0.72 +0.1 4,073,355 - Pf1N1B4_3668 0.72 +0.4 4,073,358 - Pf1N1B4_3668 0.72 -2.2 4,073,358 - Pf1N1B4_3668 0.72 -3.5 4,073,372 + Pf1N1B4_3668 0.73 -1.7 4,073,372 + Pf1N1B4_3668 0.73 -0.9 4,073,393 + Pf1N1B4_3668 0.75 -3.3 4,073,393 + Pf1N1B4_3668 0.75 +0.4 4,073,459 + Pf1N1B4_3668 0.80 -0.6 4,073,459 + Pf1N1B4_3668 0.80 -1.0 4,073,633 + -1.9 4,073,675 - -2.4 4,073,725 + -2.6 4,073,725 + -0.1 4,073,725 + -1.9 4,073,725 + -0.4 4,073,725 + -1.6 4,073,725 + -0.1 4,073,726 - -3.5 4,073,726 - -0.3 4,073,726 - -0.7 4,073,726 - +0.2 4,073,832 + -0.6 4,073,843 + -0.3 4,073,856 + +0.4 4,073,857 - -0.9 4,073,871 + -1.3 4,073,871 + -2.2 4,073,871 + -0.6 4,073,872 - -0.4 4,073,931 + -1.9 4,073,931 + +0.0 4,074,008 + Pf1N1B4_3669 0.17 +0.0 4,074,129 + +1.4 4,074,239 + Pf1N1B4_3669 0.80 -1.2 4,074,304 + +0.6 4,074,442 + -0.9 4,074,443 - +0.4
Or see this region's nucleotide sequence