Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3108 and Pf1N1B4_3109 are separated by 8 nucleotides Pf1N1B4_3109 and Pf1N1B4_3110 are separated by 212 nucleotides Pf1N1B4_3110 and Pf1N1B4_3111 overlap by 4 nucleotides
Pf1N1B4_3108 - S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284), at 3,495,058 to 3,496,170
_3108
Pf1N1B4_3109 - S-formylglutathione hydrolase (EC 3.1.2.12), at 3,496,179 to 3,497,024
_3109
Pf1N1B4_3110 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 3,497,237 to 3,497,710
_3110
Pf1N1B4_3111 - tRNA pseudouridine 13 synthase (EC 4.2.1.-), at 3,497,707 to 3,498,765
_3111
Position (kb)
3496
3497
3498 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3495.183 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.194 kb on - strand, within Pf1N1B4_3108 at 3495.194 kb on - strand at 3495.222 kb on + strand, within Pf1N1B4_3108 at 3495.322 kb on + strand, within Pf1N1B4_3108 at 3495.323 kb on - strand at 3495.323 kb on - strand, within Pf1N1B4_3108 at 3495.463 kb on + strand at 3495.463 kb on + strand, within Pf1N1B4_3108 at 3495.463 kb on + strand, within Pf1N1B4_3108 at 3495.464 kb on - strand, within Pf1N1B4_3108 at 3495.471 kb on + strand, within Pf1N1B4_3108 at 3495.529 kb on - strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.792 kb on + strand, within Pf1N1B4_3108 at 3496.036 kb on + strand, within Pf1N1B4_3108 at 3496.036 kb on + strand, within Pf1N1B4_3108 at 3496.036 kb on + strand, within Pf1N1B4_3108 at 3496.036 kb on + strand, within Pf1N1B4_3108 at 3496.037 kb on - strand, within Pf1N1B4_3108 at 3496.037 kb on - strand, within Pf1N1B4_3108 at 3496.075 kb on - strand at 3496.107 kb on + strand at 3496.108 kb on - strand at 3496.193 kb on + strand at 3496.194 kb on - strand at 3496.194 kb on - strand at 3496.194 kb on - strand at 3496.194 kb on - strand at 3496.194 kb on - strand at 3496.281 kb on + strand, within Pf1N1B4_3109 at 3496.281 kb on + strand, within Pf1N1B4_3109 at 3496.281 kb on + strand, within Pf1N1B4_3109 at 3496.282 kb on - strand, within Pf1N1B4_3109 at 3496.326 kb on + strand, within Pf1N1B4_3109 at 3496.500 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.552 kb on - strand at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.960 kb on - strand at 3497.014 kb on + strand at 3497.022 kb on + strand at 3497.181 kb on + strand at 3497.181 kb on + strand at 3497.182 kb on - strand at 3497.200 kb on + strand at 3497.200 kb on + strand at 3497.201 kb on - strand at 3497.201 kb on - strand at 3497.201 kb on - strand at 3497.741 kb on - strand at 3497.811 kb on + strand at 3497.933 kb on - strand, within Pf1N1B4_3111
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 3,495,183 + Pf1N1B4_3108 0.11 -1.2 3,495,193 + Pf1N1B4_3108 0.12 -1.1 3,495,193 + Pf1N1B4_3108 0.12 -1.7 3,495,193 + Pf1N1B4_3108 0.12 +0.6 3,495,193 + Pf1N1B4_3108 0.12 -2.8 3,495,193 + Pf1N1B4_3108 0.12 -2.4 3,495,193 + Pf1N1B4_3108 0.12 -0.1 3,495,194 - Pf1N1B4_3108 0.12 +0.6 3,495,194 - +2.2 3,495,222 + Pf1N1B4_3108 0.15 -0.1 3,495,322 + Pf1N1B4_3108 0.24 -0.6 3,495,323 - -0.4 3,495,323 - Pf1N1B4_3108 0.24 +0.5 3,495,463 + -0.4 3,495,463 + Pf1N1B4_3108 0.36 -1.4 3,495,463 + Pf1N1B4_3108 0.36 +0.6 3,495,464 - Pf1N1B4_3108 0.36 -1.7 3,495,471 + Pf1N1B4_3108 0.37 +1.1 3,495,529 - Pf1N1B4_3108 0.42 -1.7 3,495,551 + Pf1N1B4_3108 0.44 -0.4 3,495,551 + Pf1N1B4_3108 0.44 -1.4 3,495,551 + Pf1N1B4_3108 0.44 -0.7 3,495,551 + Pf1N1B4_3108 0.44 +0.6 3,495,551 + Pf1N1B4_3108 0.44 -1.9 3,495,551 + Pf1N1B4_3108 0.44 +2.0 3,495,792 + Pf1N1B4_3108 0.66 +0.6 3,496,036 + Pf1N1B4_3108 0.88 -0.9 3,496,036 + Pf1N1B4_3108 0.88 +0.2 3,496,036 + Pf1N1B4_3108 0.88 +0.2 3,496,036 + Pf1N1B4_3108 0.88 -0.6 3,496,037 - Pf1N1B4_3108 0.88 -0.6 3,496,037 - Pf1N1B4_3108 0.88 -2.2 3,496,075 - +0.6 3,496,107 + +0.6 3,496,108 - -1.2 3,496,193 + -1.4 3,496,194 - -1.9 3,496,194 - -2.2 3,496,194 - +0.3 3,496,194 - +0.1 3,496,194 - -1.7 3,496,281 + Pf1N1B4_3109 0.12 -2.9 3,496,281 + Pf1N1B4_3109 0.12 -1.4 3,496,281 + Pf1N1B4_3109 0.12 +0.3 3,496,282 - Pf1N1B4_3109 0.12 +0.6 3,496,326 + Pf1N1B4_3109 0.17 +0.1 3,496,500 + Pf1N1B4_3109 0.38 +0.6 3,496,551 + Pf1N1B4_3109 0.44 -0.4 3,496,551 + -0.9 3,496,551 + Pf1N1B4_3109 0.44 +1.0 3,496,551 + Pf1N1B4_3109 0.44 -1.1 3,496,551 + Pf1N1B4_3109 0.44 -2.2 3,496,551 + Pf1N1B4_3109 0.44 -0.9 3,496,551 + Pf1N1B4_3109 0.44 -2.2 3,496,551 + Pf1N1B4_3109 0.44 -0.4 3,496,551 + -0.9 3,496,551 + Pf1N1B4_3109 0.44 -1.4 3,496,552 - +1.6 3,496,552 - Pf1N1B4_3109 0.44 +1.2 3,496,552 - Pf1N1B4_3109 0.44 -0.9 3,496,552 - Pf1N1B4_3109 0.44 -0.6 3,496,552 - Pf1N1B4_3109 0.44 +0.1 3,496,552 - Pf1N1B4_3109 0.44 +1.6 3,496,552 - Pf1N1B4_3109 0.44 -4.1 3,496,552 - Pf1N1B4_3109 0.44 -0.4 3,496,552 - Pf1N1B4_3109 0.44 -2.5 3,496,552 - Pf1N1B4_3109 0.44 -0.6 3,496,959 + -1.0 3,496,959 + -0.8 3,496,959 + +0.6 3,496,959 + -2.4 3,496,959 + -1.5 3,496,959 + -1.4 3,496,959 + +1.1 3,496,959 + -0.8 3,496,960 - +1.5 3,497,014 + -0.5 3,497,022 + -0.9 3,497,181 + -0.4 3,497,181 + -2.8 3,497,182 - -1.7 3,497,200 + +0.4 3,497,200 + +1.1 3,497,201 - -0.4 3,497,201 - -0.9 3,497,201 - +2.2 3,497,741 - +0.4 3,497,811 + +1.0 3,497,933 - Pf1N1B4_3111 0.21 -0.4
Or see this region's nucleotide sequence