Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3011 and Pf1N1B4_3012 are separated by 165 nucleotides Pf1N1B4_3012 and Pf1N1B4_3013 are separated by 79 nucleotides
Pf1N1B4_3011 - Tyrosine recombinase XerD, at 3,385,335 to 3,386,231
_3011
Pf1N1B4_3012 - Thiol:disulfide interchange protein DsbC, at 3,386,397 to 3,387,128
_3012
Pf1N1B4_3013 - Homoserine dehydrogenase (EC 1.1.1.3), at 3,387,208 to 3,388,566
_3013
Position (kb)
3386
3387
3388 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3385.536 kb on + strand, within Pf1N1B4_3011 at 3385.536 kb on + strand, within Pf1N1B4_3011 at 3385.585 kb on - strand, within Pf1N1B4_3011 at 3385.591 kb on - strand, within Pf1N1B4_3011 at 3385.667 kb on + strand, within Pf1N1B4_3011 at 3385.668 kb on - strand, within Pf1N1B4_3011 at 3385.702 kb on + strand, within Pf1N1B4_3011 at 3385.912 kb on - strand, within Pf1N1B4_3011 at 3385.912 kb on - strand, within Pf1N1B4_3011 at 3386.169 kb on + strand at 3386.169 kb on + strand at 3386.169 kb on + strand at 3386.169 kb on + strand at 3386.169 kb on + strand at 3386.170 kb on - strand at 3386.170 kb on - strand at 3386.170 kb on - strand at 3386.170 kb on - strand at 3386.263 kb on - strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.304 kb on + strand at 3386.556 kb on + strand, within Pf1N1B4_3012 at 3386.610 kb on + strand, within Pf1N1B4_3012 at 3386.610 kb on + strand, within Pf1N1B4_3012 at 3386.610 kb on + strand, within Pf1N1B4_3012 at 3386.853 kb on + strand, within Pf1N1B4_3012 at 3386.853 kb on + strand, within Pf1N1B4_3012 at 3386.853 kb on + strand, within Pf1N1B4_3012 at 3386.853 kb on + strand, within Pf1N1B4_3012 at 3387.038 kb on + strand, within Pf1N1B4_3012 at 3387.038 kb on + strand, within Pf1N1B4_3012 at 3387.207 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 3,385,536 + Pf1N1B4_3011 0.22 +0.1 3,385,536 + Pf1N1B4_3011 0.22 -1.5 3,385,585 - Pf1N1B4_3011 0.28 -3.2 3,385,591 - Pf1N1B4_3011 0.29 -0.9 3,385,667 + Pf1N1B4_3011 0.37 -0.3 3,385,668 - Pf1N1B4_3011 0.37 -0.3 3,385,702 + Pf1N1B4_3011 0.41 -0.9 3,385,912 - Pf1N1B4_3011 0.64 +0.9 3,385,912 - Pf1N1B4_3011 0.64 +0.3 3,386,169 + -2.0 3,386,169 + -0.2 3,386,169 + -1.6 3,386,169 + +0.7 3,386,169 + -0.6 3,386,170 - +1.1 3,386,170 - -0.6 3,386,170 - -0.6 3,386,170 - -2.0 3,386,263 - -2.9 3,386,297 + +0.2 3,386,297 + +1.1 3,386,297 + +0.1 3,386,297 + +0.3 3,386,297 + -0.5 3,386,297 + -1.3 3,386,297 + -0.3 3,386,297 + +1.1 3,386,297 + -0.1 3,386,297 + +0.7 3,386,297 + -0.5 3,386,297 + +0.9 3,386,297 + +0.1 3,386,297 + -0.3 3,386,297 + +1.3 3,386,297 + -0.3 3,386,297 + +0.1 3,386,298 - +0.3 3,386,298 - +0.0 3,386,298 - +1.1 3,386,298 - -1.1 3,386,298 - -0.4 3,386,298 - -0.2 3,386,298 - +0.7 3,386,298 - -1.3 3,386,298 - -0.8 3,386,298 - +0.7 3,386,304 + +3.0 3,386,556 + Pf1N1B4_3012 0.22 -0.6 3,386,610 + Pf1N1B4_3012 0.29 -2.1 3,386,610 + Pf1N1B4_3012 0.29 -0.3 3,386,610 + Pf1N1B4_3012 0.29 -0.3 3,386,853 + Pf1N1B4_3012 0.62 +0.1 3,386,853 + Pf1N1B4_3012 0.62 -0.6 3,386,853 + Pf1N1B4_3012 0.62 +0.4 3,386,853 + Pf1N1B4_3012 0.62 -1.8 3,387,038 + Pf1N1B4_3012 0.88 +0.4 3,387,038 + Pf1N1B4_3012 0.88 -1.5 3,387,207 + +0.7
Or see this region's nucleotide sequence