Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2973 and Pf1N1B4_2974 overlap by 4 nucleotides Pf1N1B4_2974 and Pf1N1B4_2975 are separated by 154 nucleotides Pf1N1B4_2975 and Pf1N1B4_2976 are separated by 43 nucleotides
Pf1N1B4_2973 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 3,334,257 to 3,334,721
_2973
Pf1N1B4_2974 - Phosphate regulon transcriptional regulatory protein PhoB (SphR), at 3,334,718 to 3,335,434
_2974
Pf1N1B4_2975 - Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12), at 3,335,589 to 3,336,104
_2975
Pf1N1B4_2976 - DipZ protein, at 3,336,148 to 3,337,935
_2976
Position (kb)
3335
3336
3337 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3334.750 kb on - strand at 3334.849 kb on - strand, within Pf1N1B4_2974 at 3334.929 kb on + strand, within Pf1N1B4_2974 at 3334.929 kb on + strand, within Pf1N1B4_2974 at 3334.929 kb on + strand, within Pf1N1B4_2974 at 3334.930 kb on - strand, within Pf1N1B4_2974 at 3334.930 kb on - strand, within Pf1N1B4_2974 at 3334.930 kb on - strand, within Pf1N1B4_2974 at 3334.930 kb on - strand, within Pf1N1B4_2974 at 3334.930 kb on - strand, within Pf1N1B4_2974 at 3335.130 kb on + strand, within Pf1N1B4_2974 at 3335.378 kb on - strand at 3335.494 kb on + strand at 3335.494 kb on + strand at 3335.495 kb on - strand at 3335.508 kb on + strand at 3335.544 kb on + strand at 3335.544 kb on + strand at 3335.545 kb on - strand at 3335.545 kb on - strand at 3335.656 kb on + strand, within Pf1N1B4_2975 at 3335.657 kb on - strand, within Pf1N1B4_2975 at 3335.657 kb on - strand, within Pf1N1B4_2975 at 3335.806 kb on - strand at 3335.806 kb on - strand, within Pf1N1B4_2975 at 3335.834 kb on + strand, within Pf1N1B4_2975 at 3335.835 kb on - strand, within Pf1N1B4_2975 at 3336.060 kb on + strand at 3336.102 kb on + strand at 3336.147 kb on + strand at 3336.147 kb on + strand at 3336.147 kb on + strand at 3336.147 kb on + strand at 3336.147 kb on + strand at 3336.147 kb on + strand at 3336.148 kb on - strand at 3336.148 kb on - strand at 3336.169 kb on + strand at 3336.170 kb on - strand at 3336.356 kb on - strand, within Pf1N1B4_2976 at 3336.768 kb on - strand, within Pf1N1B4_2976 at 3336.867 kb on + strand, within Pf1N1B4_2976 at 3337.081 kb on + strand, within Pf1N1B4_2976 at 3337.081 kb on + strand, within Pf1N1B4_2976 at 3337.081 kb on + strand, within Pf1N1B4_2976 at 3337.081 kb on + strand, within Pf1N1B4_2976 at 3337.082 kb on - strand, within Pf1N1B4_2976 at 3337.082 kb on - strand, within Pf1N1B4_2976
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 3,334,750 - +2.3 3,334,849 - Pf1N1B4_2974 0.18 +0.7 3,334,929 + Pf1N1B4_2974 0.29 +0.2 3,334,929 + Pf1N1B4_2974 0.29 -0.3 3,334,929 + Pf1N1B4_2974 0.29 +0.8 3,334,930 - Pf1N1B4_2974 0.30 +0.7 3,334,930 - Pf1N1B4_2974 0.30 +1.4 3,334,930 - Pf1N1B4_2974 0.30 -1.6 3,334,930 - Pf1N1B4_2974 0.30 +0.1 3,334,930 - Pf1N1B4_2974 0.30 +0.4 3,335,130 + Pf1N1B4_2974 0.57 +0.6 3,335,378 - -2.1 3,335,494 + -0.7 3,335,494 + +0.7 3,335,495 - -1.3 3,335,508 + +0.3 3,335,544 + -0.6 3,335,544 + +0.0 3,335,545 - -1.1 3,335,545 - +0.2 3,335,656 + Pf1N1B4_2975 0.13 -0.3 3,335,657 - Pf1N1B4_2975 0.13 -1.1 3,335,657 - Pf1N1B4_2975 0.13 +0.7 3,335,806 - +1.1 3,335,806 - Pf1N1B4_2975 0.42 -0.3 3,335,834 + Pf1N1B4_2975 0.47 -1.2 3,335,835 - Pf1N1B4_2975 0.48 -0.3 3,336,060 + -0.1 3,336,102 + -1.3 3,336,147 + +0.4 3,336,147 + -1.6 3,336,147 + +1.0 3,336,147 + +0.7 3,336,147 + -1.6 3,336,147 + +0.2 3,336,148 - +2.3 3,336,148 - -0.3 3,336,169 + -1.0 3,336,170 - +0.5 3,336,356 - Pf1N1B4_2976 0.12 +0.6 3,336,768 - Pf1N1B4_2976 0.35 +0.3 3,336,867 + Pf1N1B4_2976 0.40 -1.0 3,337,081 + Pf1N1B4_2976 0.52 -0.3 3,337,081 + Pf1N1B4_2976 0.52 -0.6 3,337,081 + Pf1N1B4_2976 0.52 -0.5 3,337,081 + Pf1N1B4_2976 0.52 -2.6 3,337,082 - Pf1N1B4_2976 0.52 -1.7 3,337,082 - Pf1N1B4_2976 0.52 -0.5
Or see this region's nucleotide sequence