Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2624 and Pf1N1B4_2625 are separated by 54 nucleotides Pf1N1B4_2625 and Pf1N1B4_2626 overlap by 39 nucleotides
Pf1N1B4_2624 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,963,494 to 2,964,489
_2624
Pf1N1B4_2625 - hypothetical protein, at 2,964,544 to 2,964,834
_2625
Pf1N1B4_2626 - Biotin carboxylase (EC 6.3.4.14), at 2,964,796 to 2,965,881
_2626
Position (kb)
2964
2965 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2963.628 kb on - strand, within Pf1N1B4_2624 at 2963.628 kb on - strand, within Pf1N1B4_2624 at 2963.686 kb on + strand, within Pf1N1B4_2624 at 2963.686 kb on + strand, within Pf1N1B4_2624 at 2963.686 kb on + strand, within Pf1N1B4_2624 at 2963.687 kb on - strand, within Pf1N1B4_2624 at 2963.687 kb on - strand, within Pf1N1B4_2624 at 2963.687 kb on - strand, within Pf1N1B4_2624 at 2963.687 kb on - strand, within Pf1N1B4_2624 at 2963.755 kb on - strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2964.020 kb on + strand, within Pf1N1B4_2624 at 2964.021 kb on - strand, within Pf1N1B4_2624 at 2964.021 kb on - strand, within Pf1N1B4_2624 at 2964.021 kb on - strand, within Pf1N1B4_2624 at 2964.118 kb on - strand, within Pf1N1B4_2624 at 2964.494 kb on + strand at 2964.495 kb on - strand at 2964.495 kb on - strand at 2964.542 kb on + strand at 2964.643 kb on + strand, within Pf1N1B4_2625 at 2964.644 kb on - strand, within Pf1N1B4_2625 at 2964.644 kb on - strand, within Pf1N1B4_2625 at 2964.665 kb on + strand, within Pf1N1B4_2625 at 2964.666 kb on - strand, within Pf1N1B4_2625 at 2964.666 kb on - strand, within Pf1N1B4_2625 at 2964.666 kb on - strand, within Pf1N1B4_2625 at 2964.690 kb on - strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.931 kb on + strand, within Pf1N1B4_2626 at 2964.931 kb on + strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.964 kb on + strand, within Pf1N1B4_2626 at 2964.964 kb on + strand, within Pf1N1B4_2626 at 2964.964 kb on + strand, within Pf1N1B4_2626 at 2964.965 kb on - strand, within Pf1N1B4_2626 at 2964.965 kb on - strand, within Pf1N1B4_2626 at 2964.965 kb on - strand, within Pf1N1B4_2626 at 2964.965 kb on - strand, within Pf1N1B4_2626 at 2965.072 kb on - strand, within Pf1N1B4_2626 at 2965.225 kb on + strand, within Pf1N1B4_2626 at 2965.225 kb on + strand, within Pf1N1B4_2626 at 2965.225 kb on + strand, within Pf1N1B4_2626 at 2965.226 kb on - strand, within Pf1N1B4_2626 at 2965.226 kb on - strand, within Pf1N1B4_2626 at 2965.226 kb on - strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.448 kb on - strand, within Pf1N1B4_2626 at 2965.448 kb on - strand, within Pf1N1B4_2626 at 2965.448 kb on - strand at 2965.448 kb on - strand at 2965.448 kb on - strand, within Pf1N1B4_2626 at 2965.448 kb on - strand, within Pf1N1B4_2626 at 2965.513 kb on + strand, within Pf1N1B4_2626 at 2965.514 kb on - strand, within Pf1N1B4_2626 at 2965.514 kb on - strand, within Pf1N1B4_2626 at 2965.516 kb on + strand, within Pf1N1B4_2626 at 2965.516 kb on + strand, within Pf1N1B4_2626 at 2965.516 kb on + strand, within Pf1N1B4_2626 at 2965.516 kb on + strand, within Pf1N1B4_2626 at 2965.517 kb on - strand, within Pf1N1B4_2626 at 2965.517 kb on - strand, within Pf1N1B4_2626 at 2965.517 kb on - strand, within Pf1N1B4_2626 at 2965.618 kb on + strand, within Pf1N1B4_2626 at 2965.618 kb on + strand, within Pf1N1B4_2626 at 2965.618 kb on + strand at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.798 kb on + strand at 2965.798 kb on + strand at 2965.799 kb on - strand at 2965.799 kb on - strand at 2965.799 kb on - strand at 2965.799 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 2,963,628 - Pf1N1B4_2624 0.13 -1.0 2,963,628 - Pf1N1B4_2624 0.13 -0.6 2,963,686 + Pf1N1B4_2624 0.19 -0.5 2,963,686 + Pf1N1B4_2624 0.19 +1.7 2,963,686 + Pf1N1B4_2624 0.19 +0.3 2,963,687 - Pf1N1B4_2624 0.19 -1.8 2,963,687 - Pf1N1B4_2624 0.19 +0.1 2,963,687 - Pf1N1B4_2624 0.19 +1.6 2,963,687 - Pf1N1B4_2624 0.19 -0.8 2,963,755 - Pf1N1B4_2624 0.26 +1.4 2,963,844 + Pf1N1B4_2624 0.35 +2.3 2,963,844 + Pf1N1B4_2624 0.35 +1.3 2,963,844 + Pf1N1B4_2624 0.35 +0.3 2,963,844 + Pf1N1B4_2624 0.35 -0.8 2,963,844 + Pf1N1B4_2624 0.35 -2.5 2,963,844 + Pf1N1B4_2624 0.35 -1.5 2,963,844 + Pf1N1B4_2624 0.35 -1.2 2,963,845 - Pf1N1B4_2624 0.35 +0.6 2,963,845 - Pf1N1B4_2624 0.35 -1.4 2,963,845 - Pf1N1B4_2624 0.35 +0.9 2,963,845 - Pf1N1B4_2624 0.35 +0.5 2,963,845 - Pf1N1B4_2624 0.35 +2.0 2,963,845 - Pf1N1B4_2624 0.35 -0.6 2,963,845 - Pf1N1B4_2624 0.35 -2.2 2,964,020 + Pf1N1B4_2624 0.53 -1.6 2,964,021 - Pf1N1B4_2624 0.53 -0.2 2,964,021 - Pf1N1B4_2624 0.53 -0.7 2,964,021 - Pf1N1B4_2624 0.53 +0.2 2,964,118 - Pf1N1B4_2624 0.63 +0.2 2,964,494 + -0.2 2,964,495 - +0.6 2,964,495 - +0.8 2,964,542 + -0.5 2,964,643 + Pf1N1B4_2625 0.34 +1.8 2,964,644 - Pf1N1B4_2625 0.34 -0.2 2,964,644 - Pf1N1B4_2625 0.34 -1.2 2,964,665 + Pf1N1B4_2625 0.42 -0.6 2,964,666 - Pf1N1B4_2625 0.42 -0.8 2,964,666 - Pf1N1B4_2625 0.42 -0.7 2,964,666 - Pf1N1B4_2625 0.42 -2.8 2,964,690 - Pf1N1B4_2625 0.50 -1.4 2,964,763 + Pf1N1B4_2625 0.75 -1.2 2,964,763 + Pf1N1B4_2625 0.75 -1.8 2,964,763 + Pf1N1B4_2625 0.75 -0.0 2,964,763 + Pf1N1B4_2625 0.75 -0.6 2,964,763 + Pf1N1B4_2625 0.75 -0.4 2,964,764 - Pf1N1B4_2625 0.76 +1.8 2,964,764 - Pf1N1B4_2625 0.76 +1.2 2,964,764 - Pf1N1B4_2625 0.76 -0.4 2,964,764 - Pf1N1B4_2625 0.76 -0.2 2,964,764 - Pf1N1B4_2625 0.76 -1.1 2,964,931 + Pf1N1B4_2626 0.12 +1.1 2,964,931 + Pf1N1B4_2626 0.12 +0.3 2,964,932 - Pf1N1B4_2626 0.13 -0.8 2,964,932 - Pf1N1B4_2626 0.13 -1.2 2,964,932 - Pf1N1B4_2626 0.13 +0.8 2,964,932 - Pf1N1B4_2626 0.13 -1.2 2,964,932 - Pf1N1B4_2626 0.13 -0.8 2,964,964 + Pf1N1B4_2626 0.15 +1.8 2,964,964 + Pf1N1B4_2626 0.15 +1.3 2,964,964 + Pf1N1B4_2626 0.15 +0.7 2,964,965 - Pf1N1B4_2626 0.16 -0.2 2,964,965 - Pf1N1B4_2626 0.16 -2.2 2,964,965 - Pf1N1B4_2626 0.16 +0.1 2,964,965 - Pf1N1B4_2626 0.16 -0.2 2,965,072 - Pf1N1B4_2626 0.25 -2.4 2,965,225 + Pf1N1B4_2626 0.40 +0.5 2,965,225 + Pf1N1B4_2626 0.40 +0.1 2,965,225 + Pf1N1B4_2626 0.40 +0.7 2,965,226 - Pf1N1B4_2626 0.40 -0.3 2,965,226 - Pf1N1B4_2626 0.40 +0.2 2,965,226 - Pf1N1B4_2626 0.40 +0.1 2,965,447 + Pf1N1B4_2626 0.60 +0.8 2,965,447 + Pf1N1B4_2626 0.60 +0.2 2,965,447 + Pf1N1B4_2626 0.60 -1.5 2,965,447 + Pf1N1B4_2626 0.60 +1.4 2,965,447 + Pf1N1B4_2626 0.60 -0.4 2,965,447 + Pf1N1B4_2626 0.60 +1.5 2,965,447 + Pf1N1B4_2626 0.60 -0.2 2,965,448 - Pf1N1B4_2626 0.60 +1.6 2,965,448 - Pf1N1B4_2626 0.60 -1.8 2,965,448 - +2.4 2,965,448 - -0.8 2,965,448 - Pf1N1B4_2626 0.60 +1.6 2,965,448 - Pf1N1B4_2626 0.60 -0.2 2,965,513 + Pf1N1B4_2626 0.66 -0.8 2,965,514 - Pf1N1B4_2626 0.66 +0.1 2,965,514 - Pf1N1B4_2626 0.66 -2.5 2,965,516 + Pf1N1B4_2626 0.66 +0.6 2,965,516 + Pf1N1B4_2626 0.66 -1.4 2,965,516 + Pf1N1B4_2626 0.66 -1.3 2,965,516 + Pf1N1B4_2626 0.66 -1.1 2,965,517 - Pf1N1B4_2626 0.66 -0.9 2,965,517 - Pf1N1B4_2626 0.66 -1.4 2,965,517 - Pf1N1B4_2626 0.66 -1.9 2,965,618 + Pf1N1B4_2626 0.76 -0.8 2,965,618 + Pf1N1B4_2626 0.76 -0.8 2,965,618 + +1.0 2,965,636 + Pf1N1B4_2626 0.77 +1.2 2,965,636 + Pf1N1B4_2626 0.77 +0.3 2,965,636 + Pf1N1B4_2626 0.77 +0.5 2,965,636 + Pf1N1B4_2626 0.77 -0.6 2,965,636 + Pf1N1B4_2626 0.77 -0.5 2,965,637 - Pf1N1B4_2626 0.77 -0.8 2,965,637 - Pf1N1B4_2626 0.77 -0.8 2,965,637 - Pf1N1B4_2626 0.77 +0.5 2,965,637 - Pf1N1B4_2626 0.77 +1.1 2,965,637 - Pf1N1B4_2626 0.77 -0.4 2,965,798 + -0.4 2,965,798 + -0.3 2,965,799 - -0.2 2,965,799 - -0.8 2,965,799 - +1.7 2,965,799 - -1.5
Or see this region's nucleotide sequence