Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2432 and Pf1N1B4_2433 are separated by 137 nucleotides Pf1N1B4_2433 and Pf1N1B4_2434 are separated by 32 nucleotides Pf1N1B4_2434 and Pf1N1B4_2435 are separated by 40 nucleotides Pf1N1B4_2435 and Pf1N1B4_2436 are separated by 306 nucleotides
Pf1N1B4_2432 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,757,405 to 2,758,349
_2432
Pf1N1B4_2433 - FIG00953108: hypothetical protein, at 2,758,487 to 2,758,903
_2433
Pf1N1B4_2434 - putative DNA binding protein, at 2,758,936 to 2,759,202
_2434
Pf1N1B4_2435 - hypothetical protein, at 2,759,243 to 2,759,395
_2435
Pf1N1B4_2436 - FIG00953563: hypothetical protein, at 2,759,702 to 2,760,103
_2436
Position (kb)
2758
2759 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2757.510 kb on + strand, within Pf1N1B4_2432 at 2757.619 kb on + strand, within Pf1N1B4_2432 at 2757.811 kb on - strand, within Pf1N1B4_2432 at 2758.088 kb on + strand, within Pf1N1B4_2432 at 2758.132 kb on + strand, within Pf1N1B4_2432 at 2758.132 kb on + strand, within Pf1N1B4_2432 at 2758.180 kb on - strand, within Pf1N1B4_2432 at 2758.279 kb on - strand at 2758.348 kb on - strand at 2758.488 kb on + strand at 2758.489 kb on - strand at 2758.521 kb on + strand at 2758.522 kb on - strand at 2758.522 kb on - strand at 2758.522 kb on - strand at 2758.537 kb on - strand, within Pf1N1B4_2433 at 2758.537 kb on - strand, within Pf1N1B4_2433 at 2758.660 kb on + strand, within Pf1N1B4_2433 at 2758.660 kb on + strand, within Pf1N1B4_2433 at 2758.661 kb on - strand, within Pf1N1B4_2433 at 2758.661 kb on - strand, within Pf1N1B4_2433 at 2758.661 kb on - strand, within Pf1N1B4_2433 at 2758.661 kb on - strand, within Pf1N1B4_2433 at 2758.937 kb on + strand at 2758.937 kb on + strand at 2758.937 kb on + strand at 2758.938 kb on - strand at 2758.938 kb on - strand at 2758.945 kb on + strand at 2758.945 kb on + strand at 2758.945 kb on + strand at 2759.076 kb on + strand, within Pf1N1B4_2434 at 2759.077 kb on - strand, within Pf1N1B4_2434 at 2759.180 kb on + strand at 2759.180 kb on + strand at 2759.180 kb on + strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.181 kb on - strand at 2759.247 kb on + strand at 2759.248 kb on - strand at 2759.248 kb on - strand at 2759.248 kb on - strand at 2759.248 kb on - strand at 2759.281 kb on + strand, within Pf1N1B4_2435 at 2759.317 kb on + strand, within Pf1N1B4_2435 at 2759.317 kb on + strand, within Pf1N1B4_2435 at 2759.329 kb on + strand, within Pf1N1B4_2435 at 2759.352 kb on + strand, within Pf1N1B4_2435 at 2759.353 kb on - strand, within Pf1N1B4_2435 at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.384 kb on + strand at 2759.385 kb on - strand at 2759.503 kb on + strand at 2759.590 kb on + strand at 2759.622 kb on + strand at 2759.659 kb on + strand at 2759.660 kb on - strand at 2759.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 2,757,510 + Pf1N1B4_2432 0.11 -0.6 2,757,619 + Pf1N1B4_2432 0.23 -0.7 2,757,811 - Pf1N1B4_2432 0.43 +1.4 2,758,088 + Pf1N1B4_2432 0.72 -0.1 2,758,132 + Pf1N1B4_2432 0.77 +1.9 2,758,132 + Pf1N1B4_2432 0.77 -0.5 2,758,180 - Pf1N1B4_2432 0.82 -1.3 2,758,279 - -0.3 2,758,348 - -0.9 2,758,488 + +2.5 2,758,489 - -0.7 2,758,521 + -1.7 2,758,522 - -1.9 2,758,522 - -0.4 2,758,522 - -0.5 2,758,537 - Pf1N1B4_2433 0.12 -2.1 2,758,537 - Pf1N1B4_2433 0.12 +0.3 2,758,660 + Pf1N1B4_2433 0.41 -1.1 2,758,660 + Pf1N1B4_2433 0.41 -1.7 2,758,661 - Pf1N1B4_2433 0.42 +0.5 2,758,661 - Pf1N1B4_2433 0.42 -0.4 2,758,661 - Pf1N1B4_2433 0.42 +0.6 2,758,661 - Pf1N1B4_2433 0.42 -1.1 2,758,937 + -1.1 2,758,937 + -0.5 2,758,937 + -1.7 2,758,938 - -1.7 2,758,938 - -0.1 2,758,945 + -0.9 2,758,945 + -0.7 2,758,945 + -0.4 2,759,076 + Pf1N1B4_2434 0.52 +1.0 2,759,077 - Pf1N1B4_2434 0.53 +0.1 2,759,180 + +1.4 2,759,180 + +1.9 2,759,180 + -1.1 2,759,181 - +0.1 2,759,181 - -0.4 2,759,181 - +0.5 2,759,181 - +0.5 2,759,181 - +1.5 2,759,247 + -0.7 2,759,248 - -0.1 2,759,248 - +0.0 2,759,248 - -0.1 2,759,248 - +0.2 2,759,281 + Pf1N1B4_2435 0.25 +0.3 2,759,317 + Pf1N1B4_2435 0.48 +0.3 2,759,317 + Pf1N1B4_2435 0.48 +0.3 2,759,329 + Pf1N1B4_2435 0.56 -0.8 2,759,352 + Pf1N1B4_2435 0.71 -0.6 2,759,353 - Pf1N1B4_2435 0.72 +0.1 2,759,384 + +0.1 2,759,384 + -0.6 2,759,384 + -0.7 2,759,384 + -0.1 2,759,384 + -2.5 2,759,384 + -0.1 2,759,385 - +0.5 2,759,503 + +0.3 2,759,590 + +0.9 2,759,622 + -1.7 2,759,659 + -0.7 2,759,660 - -1.1 2,759,670 - +0.4
Or see this region's nucleotide sequence