Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2053 and Pf1N1B4_2054 are separated by 12 nucleotides Pf1N1B4_2054 and Pf1N1B4_2055 are separated by 18 nucleotides
Pf1N1B4_2053 - Rhs-family protein, at 2,339,640 to 2,344,013
_2053
Pf1N1B4_2054 - FIG00964469: hypothetical protein, at 2,344,026 to 2,344,460
_2054
Pf1N1B4_2055 - VgrG-3 protein, at 2,344,479 to 2,346,416
_2055
Position (kb)
2344
2345 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2343.026 kb on - strand, within Pf1N1B4_2053 at 2343.026 kb on - strand, within Pf1N1B4_2053 at 2343.381 kb on - strand, within Pf1N1B4_2053 at 2343.381 kb on - strand, within Pf1N1B4_2053 at 2343.381 kb on - strand, within Pf1N1B4_2053 at 2343.577 kb on + strand at 2343.577 kb on + strand at 2343.577 kb on + strand at 2343.577 kb on + strand at 2343.577 kb on + strand at 2343.578 kb on - strand at 2343.578 kb on - strand at 2343.578 kb on - strand at 2343.707 kb on + strand at 2343.707 kb on + strand at 2343.707 kb on + strand at 2343.708 kb on - strand at 2343.708 kb on - strand at 2343.708 kb on - strand at 2344.111 kb on + strand, within Pf1N1B4_2054 at 2344.111 kb on + strand, within Pf1N1B4_2054 at 2344.112 kb on - strand, within Pf1N1B4_2054 at 2344.112 kb on - strand, within Pf1N1B4_2054 at 2344.112 kb on - strand, within Pf1N1B4_2054 at 2344.453 kb on + strand at 2344.453 kb on + strand at 2344.454 kb on - strand at 2344.624 kb on + strand at 2344.624 kb on + strand at 2344.624 kb on + strand at 2344.625 kb on - strand at 2344.625 kb on - strand at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.729 kb on + strand, within Pf1N1B4_2055 at 2344.730 kb on - strand, within Pf1N1B4_2055 at 2344.730 kb on - strand, within Pf1N1B4_2055 at 2344.730 kb on - strand, within Pf1N1B4_2055 at 2344.730 kb on - strand, within Pf1N1B4_2055 at 2344.730 kb on - strand at 2344.730 kb on - strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.918 kb on + strand, within Pf1N1B4_2055 at 2344.919 kb on - strand, within Pf1N1B4_2055 at 2344.919 kb on - strand, within Pf1N1B4_2055 at 2345.060 kb on - strand, within Pf1N1B4_2055 at 2345.230 kb on + strand, within Pf1N1B4_2055 at 2345.230 kb on + strand, within Pf1N1B4_2055 at 2345.230 kb on + strand, within Pf1N1B4_2055 at 2345.321 kb on + strand, within Pf1N1B4_2055 at 2345.351 kb on - strand, within Pf1N1B4_2055 at 2345.440 kb on + strand, within Pf1N1B4_2055 at 2345.440 kb on + strand, within Pf1N1B4_2055 at 2345.440 kb on + strand, within Pf1N1B4_2055 at 2345.441 kb on - strand, within Pf1N1B4_2055 at 2345.441 kb on - strand, within Pf1N1B4_2055 at 2345.441 kb on - strand, within Pf1N1B4_2055 at 2345.441 kb on - strand, within Pf1N1B4_2055 at 2345.441 kb on - strand, within Pf1N1B4_2055 at 2345.443 kb on + strand, within Pf1N1B4_2055
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 2,343,026 - Pf1N1B4_2053 0.77 +0.7 2,343,026 - Pf1N1B4_2053 0.77 -1.9 2,343,381 - Pf1N1B4_2053 0.86 -0.0 2,343,381 - Pf1N1B4_2053 0.86 +0.4 2,343,381 - Pf1N1B4_2053 0.86 +0.4 2,343,577 + +1.0 2,343,577 + -0.8 2,343,577 + -0.2 2,343,577 + +1.0 2,343,577 + +0.2 2,343,578 - -0.6 2,343,578 - -0.4 2,343,578 - -0.7 2,343,707 + -0.6 2,343,707 + -0.6 2,343,707 + -2.3 2,343,708 - -1.8 2,343,708 - -0.5 2,343,708 - -0.4 2,344,111 + Pf1N1B4_2054 0.20 -0.7 2,344,111 + Pf1N1B4_2054 0.20 -0.5 2,344,112 - Pf1N1B4_2054 0.20 -1.6 2,344,112 - Pf1N1B4_2054 0.20 -1.6 2,344,112 - Pf1N1B4_2054 0.20 -1.4 2,344,453 + -0.6 2,344,453 + +0.2 2,344,454 - +0.1 2,344,624 + +0.1 2,344,624 + +0.9 2,344,624 + -2.6 2,344,625 - -0.6 2,344,625 - -0.3 2,344,729 + Pf1N1B4_2055 0.13 -0.6 2,344,729 + Pf1N1B4_2055 0.13 -0.0 2,344,729 + Pf1N1B4_2055 0.13 +1.0 2,344,729 + -1.3 2,344,729 + Pf1N1B4_2055 0.13 +0.1 2,344,729 + Pf1N1B4_2055 0.13 +0.6 2,344,729 + Pf1N1B4_2055 0.13 +0.8 2,344,729 + Pf1N1B4_2055 0.13 -0.8 2,344,729 + Pf1N1B4_2055 0.13 +1.5 2,344,729 + Pf1N1B4_2055 0.13 -1.0 2,344,729 + Pf1N1B4_2055 0.13 +1.3 2,344,729 + Pf1N1B4_2055 0.13 +0.4 2,344,729 + Pf1N1B4_2055 0.13 +0.3 2,344,729 + Pf1N1B4_2055 0.13 +1.3 2,344,730 - Pf1N1B4_2055 0.13 -0.0 2,344,730 - Pf1N1B4_2055 0.13 -1.6 2,344,730 - Pf1N1B4_2055 0.13 +0.4 2,344,730 - Pf1N1B4_2055 0.13 +0.6 2,344,730 - +0.4 2,344,730 - Pf1N1B4_2055 0.13 -0.2 2,344,918 + Pf1N1B4_2055 0.23 +0.3 2,344,918 + Pf1N1B4_2055 0.23 +1.6 2,344,918 + Pf1N1B4_2055 0.23 -0.3 2,344,918 + Pf1N1B4_2055 0.23 -0.3 2,344,918 + Pf1N1B4_2055 0.23 -0.2 2,344,918 + Pf1N1B4_2055 0.23 +0.6 2,344,918 + Pf1N1B4_2055 0.23 +2.2 2,344,918 + Pf1N1B4_2055 0.23 +1.0 2,344,918 + Pf1N1B4_2055 0.23 -0.0 2,344,918 + Pf1N1B4_2055 0.23 -0.0 2,344,919 - Pf1N1B4_2055 0.23 +1.4 2,344,919 - Pf1N1B4_2055 0.23 -0.6 2,345,060 - Pf1N1B4_2055 0.30 -0.1 2,345,230 + Pf1N1B4_2055 0.39 +0.5 2,345,230 + Pf1N1B4_2055 0.39 +0.8 2,345,230 + Pf1N1B4_2055 0.39 -1.1 2,345,321 + Pf1N1B4_2055 0.43 -0.4 2,345,351 - Pf1N1B4_2055 0.45 +0.9 2,345,440 + Pf1N1B4_2055 0.50 +0.1 2,345,440 + Pf1N1B4_2055 0.50 +0.2 2,345,440 + Pf1N1B4_2055 0.50 +1.4 2,345,441 - Pf1N1B4_2055 0.50 -0.1 2,345,441 - Pf1N1B4_2055 0.50 -0.9 2,345,441 - Pf1N1B4_2055 0.50 -2.0 2,345,441 - Pf1N1B4_2055 0.50 +1.1 2,345,441 - Pf1N1B4_2055 0.50 +0.5 2,345,443 + Pf1N1B4_2055 0.50 +0.4
Or see this region's nucleotide sequence