Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1557 and Pf1N1B4_1558 are separated by 59 nucleotides Pf1N1B4_1558 and Pf1N1B4_1559 are separated by 270 nucleotides
Pf1N1B4_1557 - Polyhydroxyalkanoic acid synthase, at 1,796,052 to 1,797,734
_1557
Pf1N1B4_1558 - Transcriptional regulator PhaD, at 1,797,794 to 1,798,414
_1558
Pf1N1B4_1559 - Polyhydroxyalkanoate granule-associated protein PhaF, at 1,798,685 to 1,799,473
_1559
Position (kb)
1797
1798
1799 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1796.859 kb on + strand, within Pf1N1B4_1557 at 1796.860 kb on - strand at 1796.995 kb on + strand, within Pf1N1B4_1557 at 1796.995 kb on + strand, within Pf1N1B4_1557 at 1796.995 kb on + strand, within Pf1N1B4_1557 at 1796.996 kb on - strand, within Pf1N1B4_1557 at 1796.996 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.019 kb on - strand, within Pf1N1B4_1557 at 1797.046 kb on + strand, within Pf1N1B4_1557 at 1797.047 kb on - strand, within Pf1N1B4_1557 at 1797.047 kb on - strand, within Pf1N1B4_1557 at 1797.186 kb on + strand, within Pf1N1B4_1557 at 1797.186 kb on + strand, within Pf1N1B4_1557 at 1797.186 kb on + strand, within Pf1N1B4_1557 at 1797.187 kb on - strand, within Pf1N1B4_1557 at 1797.187 kb on - strand, within Pf1N1B4_1557 at 1797.197 kb on + strand, within Pf1N1B4_1557 at 1797.198 kb on - strand, within Pf1N1B4_1557 at 1797.201 kb on + strand, within Pf1N1B4_1557 at 1797.201 kb on + strand, within Pf1N1B4_1557 at 1797.201 kb on + strand, within Pf1N1B4_1557 at 1797.202 kb on - strand, within Pf1N1B4_1557 at 1797.202 kb on - strand, within Pf1N1B4_1557 at 1797.202 kb on - strand, within Pf1N1B4_1557 at 1797.241 kb on - strand, within Pf1N1B4_1557 at 1797.432 kb on + strand, within Pf1N1B4_1557 at 1797.445 kb on + strand, within Pf1N1B4_1557 at 1797.446 kb on - strand, within Pf1N1B4_1557 at 1797.446 kb on - strand, within Pf1N1B4_1557 at 1797.493 kb on + strand, within Pf1N1B4_1557 at 1797.493 kb on + strand, within Pf1N1B4_1557 at 1797.493 kb on + strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.494 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.535 kb on - strand, within Pf1N1B4_1557 at 1797.576 kb on + strand at 1797.576 kb on + strand at 1797.579 kb on + strand at 1797.580 kb on - strand at 1797.580 kb on - strand at 1797.580 kb on - strand at 1797.580 kb on - strand at 1797.603 kb on - strand at 1797.687 kb on + strand at 1797.688 kb on - strand at 1797.714 kb on - strand at 1797.717 kb on + strand at 1797.717 kb on + strand at 1797.717 kb on + strand at 1797.717 kb on + strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.740 kb on - strand at 1797.782 kb on + strand at 1797.782 kb on + strand at 1797.782 kb on + strand at 1797.911 kb on + strand, within Pf1N1B4_1558 at 1797.912 kb on - strand, within Pf1N1B4_1558 at 1797.912 kb on - strand at 1797.915 kb on - strand, within Pf1N1B4_1558 at 1798.026 kb on - strand, within Pf1N1B4_1558 at 1798.275 kb on - strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.445 kb on + strand at 1798.445 kb on + strand at 1798.446 kb on - strand at 1798.526 kb on + strand at 1798.526 kb on + strand at 1798.526 kb on + strand at 1798.526 kb on + strand at 1798.782 kb on - strand, within Pf1N1B4_1559 at 1798.904 kb on - strand, within Pf1N1B4_1559 at 1798.939 kb on + strand, within Pf1N1B4_1559 at 1799.339 kb on - strand, within Pf1N1B4_1559 at 1799.385 kb on - strand, within Pf1N1B4_1559 at 1799.413 kb on - strand at 1799.413 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 1,796,859 + Pf1N1B4_1557 0.48 +1.4 1,796,860 - +0.9 1,796,995 + Pf1N1B4_1557 0.56 +1.5 1,796,995 + Pf1N1B4_1557 0.56 +0.9 1,796,995 + Pf1N1B4_1557 0.56 -0.5 1,796,996 - Pf1N1B4_1557 0.56 -0.9 1,796,996 - Pf1N1B4_1557 0.56 +0.2 1,797,019 - Pf1N1B4_1557 0.57 +0.0 1,797,019 - Pf1N1B4_1557 0.57 +0.2 1,797,019 - Pf1N1B4_1557 0.57 +0.6 1,797,019 - Pf1N1B4_1557 0.57 -1.9 1,797,019 - Pf1N1B4_1557 0.57 +1.7 1,797,019 - Pf1N1B4_1557 0.57 +1.9 1,797,046 + Pf1N1B4_1557 0.59 -0.7 1,797,047 - Pf1N1B4_1557 0.59 +1.3 1,797,047 - Pf1N1B4_1557 0.59 +1.3 1,797,186 + Pf1N1B4_1557 0.67 -0.2 1,797,186 + Pf1N1B4_1557 0.67 -0.3 1,797,186 + Pf1N1B4_1557 0.67 +1.2 1,797,187 - Pf1N1B4_1557 0.67 -2.1 1,797,187 - Pf1N1B4_1557 0.67 +0.6 1,797,197 + Pf1N1B4_1557 0.68 -0.7 1,797,198 - Pf1N1B4_1557 0.68 -1.1 1,797,201 + Pf1N1B4_1557 0.68 -1.5 1,797,201 + Pf1N1B4_1557 0.68 +1.2 1,797,201 + Pf1N1B4_1557 0.68 +0.7 1,797,202 - Pf1N1B4_1557 0.68 -0.2 1,797,202 - Pf1N1B4_1557 0.68 -2.4 1,797,202 - Pf1N1B4_1557 0.68 +0.2 1,797,241 - Pf1N1B4_1557 0.71 -1.0 1,797,432 + Pf1N1B4_1557 0.82 +0.7 1,797,445 + Pf1N1B4_1557 0.83 -0.3 1,797,446 - Pf1N1B4_1557 0.83 +2.5 1,797,446 - Pf1N1B4_1557 0.83 -1.1 1,797,493 + Pf1N1B4_1557 0.86 -0.1 1,797,493 + Pf1N1B4_1557 0.86 +0.4 1,797,493 + Pf1N1B4_1557 0.86 -1.1 1,797,494 - Pf1N1B4_1557 0.86 +0.1 1,797,494 - Pf1N1B4_1557 0.86 -0.4 1,797,494 - Pf1N1B4_1557 0.86 -0.3 1,797,494 - Pf1N1B4_1557 0.86 +0.9 1,797,494 - Pf1N1B4_1557 0.86 -0.2 1,797,494 - Pf1N1B4_1557 0.86 +0.7 1,797,494 - Pf1N1B4_1557 0.86 +0.3 1,797,535 - Pf1N1B4_1557 0.88 +0.6 1,797,535 - Pf1N1B4_1557 0.88 -1.9 1,797,535 - Pf1N1B4_1557 0.88 +1.2 1,797,535 - Pf1N1B4_1557 0.88 -0.1 1,797,535 - Pf1N1B4_1557 0.88 +0.4 1,797,535 - Pf1N1B4_1557 0.88 -0.5 1,797,535 - Pf1N1B4_1557 0.88 +0.2 1,797,535 - Pf1N1B4_1557 0.88 +0.3 1,797,576 + -1.7 1,797,576 + -0.5 1,797,579 + -1.2 1,797,580 - +1.6 1,797,580 - +0.1 1,797,580 - +0.9 1,797,580 - +0.2 1,797,603 - +0.6 1,797,687 + +0.3 1,797,688 - +1.8 1,797,714 - +0.9 1,797,717 + -2.5 1,797,717 + -2.1 1,797,717 + -1.5 1,797,717 + -0.7 1,797,718 - -0.7 1,797,718 - -1.4 1,797,718 - +0.8 1,797,718 - +0.9 1,797,718 - +0.3 1,797,718 - +2.5 1,797,718 - +0.0 1,797,718 - +0.7 1,797,740 - -0.4 1,797,782 + -0.7 1,797,782 + +0.1 1,797,782 + -0.5 1,797,911 + Pf1N1B4_1558 0.19 -1.4 1,797,912 - Pf1N1B4_1558 0.19 -0.4 1,797,912 - -0.7 1,797,915 - Pf1N1B4_1558 0.19 -0.9 1,798,026 - Pf1N1B4_1558 0.37 -0.1 1,798,275 - Pf1N1B4_1558 0.77 +0.4 1,798,319 + Pf1N1B4_1558 0.85 -1.1 1,798,319 + Pf1N1B4_1558 0.85 -0.4 1,798,319 + Pf1N1B4_1558 0.85 -1.9 1,798,319 + Pf1N1B4_1558 0.85 -0.4 1,798,445 + +0.2 1,798,445 + +1.5 1,798,446 - -0.2 1,798,526 + -1.1 1,798,526 + +0.1 1,798,526 + +1.1 1,798,526 + -0.3 1,798,782 - Pf1N1B4_1559 0.12 +1.7 1,798,904 - Pf1N1B4_1559 0.28 -0.1 1,798,939 + Pf1N1B4_1559 0.32 +0.9 1,799,339 - Pf1N1B4_1559 0.83 -0.1 1,799,385 - Pf1N1B4_1559 0.89 -0.4 1,799,413 - +0.9 1,799,413 - +0.9
Or see this region's nucleotide sequence