Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1479 and Pf1N1B4_1480 are separated by 208 nucleotides Pf1N1B4_1480 and Pf1N1B4_1481 are separated by 83 nucleotides
Pf1N1B4_1479 - Sensory box/GGDEF family protein, at 1,698,984 to 1,701,677
_1479
Pf1N1B4_1480 - Peptide methionine sulfoxide reductase MsrA (EC 1.8.4.11), at 1,701,886 to 1,702,533
_1480
Pf1N1B4_1481 - Protein involved in catabolism of external DNA, at 1,702,617 to 1,703,453
_1481
Position (kb)
1701
1702
1703 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1700.893 kb on - strand, within Pf1N1B4_1479 at 1700.893 kb on - strand, within Pf1N1B4_1479 at 1700.955 kb on + strand, within Pf1N1B4_1479 at 1700.956 kb on - strand, within Pf1N1B4_1479 at 1700.956 kb on - strand, within Pf1N1B4_1479 at 1700.956 kb on - strand, within Pf1N1B4_1479 at 1700.956 kb on - strand, within Pf1N1B4_1479 at 1701.396 kb on + strand, within Pf1N1B4_1479 at 1701.397 kb on - strand, within Pf1N1B4_1479 at 1701.397 kb on - strand, within Pf1N1B4_1479 at 1701.600 kb on + strand at 1701.810 kb on + strand at 1701.810 kb on + strand at 1701.817 kb on + strand at 1701.852 kb on + strand at 1701.852 kb on + strand at 1701.852 kb on + strand at 1701.852 kb on + strand at 1701.852 kb on + strand at 1701.853 kb on - strand at 1701.853 kb on - strand at 1701.856 kb on + strand at 1701.856 kb on + strand at 1701.856 kb on + strand at 1701.856 kb on + strand at 1701.857 kb on - strand at 1701.857 kb on - strand at 1701.857 kb on - strand at 1701.857 kb on - strand at 1702.150 kb on + strand, within Pf1N1B4_1480 at 1702.150 kb on + strand, within Pf1N1B4_1480 at 1702.151 kb on - strand, within Pf1N1B4_1480 at 1702.151 kb on - strand, within Pf1N1B4_1480 at 1702.204 kb on + strand, within Pf1N1B4_1480 at 1702.226 kb on - strand, within Pf1N1B4_1480 at 1702.321 kb on + strand, within Pf1N1B4_1480 at 1702.322 kb on - strand, within Pf1N1B4_1480 at 1702.322 kb on - strand, within Pf1N1B4_1480 at 1702.441 kb on + strand, within Pf1N1B4_1480 at 1702.441 kb on + strand, within Pf1N1B4_1480 at 1702.441 kb on + strand, within Pf1N1B4_1480 at 1702.442 kb on - strand, within Pf1N1B4_1480 at 1702.442 kb on - strand at 1702.442 kb on - strand, within Pf1N1B4_1480 at 1702.442 kb on - strand, within Pf1N1B4_1480 at 1702.442 kb on - strand, within Pf1N1B4_1480 at 1702.456 kb on + strand, within Pf1N1B4_1480 at 1702.468 kb on + strand, within Pf1N1B4_1480 at 1702.468 kb on + strand at 1702.469 kb on - strand at 1702.527 kb on + strand at 1702.527 kb on + strand at 1702.527 kb on + strand at 1702.527 kb on + strand at 1702.527 kb on + strand at 1702.528 kb on - strand at 1702.528 kb on - strand at 1702.528 kb on - strand at 1702.528 kb on - strand at 1702.529 kb on + strand at 1702.529 kb on + strand at 1702.529 kb on + strand at 1702.529 kb on + strand at 1702.529 kb on + strand at 1702.529 kb on + strand at 1702.529 kb on + strand at 1702.529 kb on + strand at 1702.529 kb on + strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.531 kb on + strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.532 kb on - strand at 1702.598 kb on - strand at 1702.598 kb on - strand at 1702.618 kb on + strand at 1702.757 kb on + strand, within Pf1N1B4_1481 at 1702.834 kb on + strand, within Pf1N1B4_1481 at 1703.314 kb on + strand, within Pf1N1B4_1481 at 1703.314 kb on + strand, within Pf1N1B4_1481 at 1703.314 kb on + strand, within Pf1N1B4_1481 at 1703.314 kb on + strand, within Pf1N1B4_1481 at 1703.315 kb on - strand at 1703.345 kb on - strand, within Pf1N1B4_1481 at 1703.345 kb on - strand, within Pf1N1B4_1481 at 1703.446 kb on + strand at 1703.446 kb on + strand at 1703.447 kb on - strand at 1703.481 kb on + strand at 1703.481 kb on + strand at 1703.481 kb on + strand at 1703.482 kb on - strand at 1703.482 kb on - strand at 1703.482 kb on - strand at 1703.488 kb on + strand at 1703.489 kb on - strand at 1703.526 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 1,700,893 - Pf1N1B4_1479 0.71 +1.2 1,700,893 - Pf1N1B4_1479 0.71 -0.7 1,700,955 + Pf1N1B4_1479 0.73 -0.4 1,700,956 - Pf1N1B4_1479 0.73 -2.3 1,700,956 - Pf1N1B4_1479 0.73 +1.1 1,700,956 - Pf1N1B4_1479 0.73 +0.7 1,700,956 - Pf1N1B4_1479 0.73 -0.4 1,701,396 + Pf1N1B4_1479 0.90 +0.2 1,701,397 - Pf1N1B4_1479 0.90 -0.4 1,701,397 - Pf1N1B4_1479 0.90 -0.6 1,701,600 + +0.6 1,701,810 + -0.5 1,701,810 + +1.9 1,701,817 + +0.6 1,701,852 + -0.7 1,701,852 + -0.7 1,701,852 + -0.8 1,701,852 + -0.4 1,701,852 + +1.7 1,701,853 - -2.3 1,701,853 - +0.5 1,701,856 + -1.3 1,701,856 + +0.9 1,701,856 + -0.6 1,701,856 + -0.1 1,701,857 - -0.7 1,701,857 - +0.4 1,701,857 - -1.7 1,701,857 - -2.7 1,702,150 + Pf1N1B4_1480 0.41 -0.9 1,702,150 + Pf1N1B4_1480 0.41 +0.9 1,702,151 - Pf1N1B4_1480 0.41 -1.9 1,702,151 - Pf1N1B4_1480 0.41 -1.6 1,702,204 + Pf1N1B4_1480 0.49 -0.3 1,702,226 - Pf1N1B4_1480 0.52 -0.3 1,702,321 + Pf1N1B4_1480 0.67 -0.3 1,702,322 - Pf1N1B4_1480 0.67 -0.7 1,702,322 - Pf1N1B4_1480 0.67 -1.4 1,702,441 + Pf1N1B4_1480 0.86 -1.4 1,702,441 + Pf1N1B4_1480 0.86 +0.0 1,702,441 + Pf1N1B4_1480 0.86 -1.4 1,702,442 - Pf1N1B4_1480 0.86 -0.1 1,702,442 - +0.3 1,702,442 - Pf1N1B4_1480 0.86 -1.9 1,702,442 - Pf1N1B4_1480 0.86 +1.5 1,702,442 - Pf1N1B4_1480 0.86 -0.1 1,702,456 + Pf1N1B4_1480 0.88 -0.1 1,702,468 + Pf1N1B4_1480 0.90 -0.6 1,702,468 + -0.7 1,702,469 - -1.4 1,702,527 + +0.0 1,702,527 + +0.3 1,702,527 + -0.9 1,702,527 + -0.0 1,702,527 + -0.1 1,702,528 - -2.1 1,702,528 - -0.5 1,702,528 - -0.1 1,702,528 - +0.2 1,702,529 + +0.2 1,702,529 + +1.6 1,702,529 + +0.5 1,702,529 + -0.3 1,702,529 + +0.9 1,702,529 + +1.0 1,702,529 + -0.1 1,702,529 + +0.4 1,702,529 + -1.3 1,702,530 - -1.1 1,702,530 - +0.9 1,702,530 - +1.6 1,702,530 - -0.1 1,702,530 - +0.1 1,702,530 - -0.4 1,702,530 - -1.7 1,702,530 - +1.1 1,702,530 - -1.4 1,702,530 - +0.2 1,702,530 - +0.2 1,702,530 - +0.2 1,702,530 - +1.4 1,702,530 - -0.1 1,702,530 - -1.7 1,702,530 - -0.4 1,702,531 + -0.1 1,702,531 + +0.4 1,702,531 + -0.1 1,702,531 + -0.1 1,702,531 + -0.1 1,702,531 + -0.7 1,702,531 + -1.6 1,702,531 + -0.1 1,702,531 + +1.9 1,702,531 + +0.1 1,702,531 + -1.7 1,702,531 + -0.7 1,702,531 + +0.5 1,702,531 + +0.2 1,702,531 + +0.5 1,702,531 + +0.7 1,702,531 + -0.2 1,702,531 + -2.1 1,702,532 - -2.9 1,702,532 - +0.3 1,702,532 - -1.8 1,702,532 - +0.0 1,702,532 - +0.3 1,702,532 - +0.4 1,702,532 - -0.2 1,702,532 - +0.2 1,702,532 - +0.3 1,702,532 - +1.1 1,702,532 - -0.7 1,702,532 - -0.2 1,702,532 - +0.2 1,702,532 - -0.4 1,702,598 - +0.2 1,702,598 - -0.1 1,702,618 + +0.9 1,702,757 + Pf1N1B4_1481 0.17 +0.4 1,702,834 + Pf1N1B4_1481 0.26 -0.4 1,703,314 + Pf1N1B4_1481 0.83 +0.2 1,703,314 + Pf1N1B4_1481 0.83 +0.3 1,703,314 + Pf1N1B4_1481 0.83 -1.1 1,703,314 + Pf1N1B4_1481 0.83 +0.4 1,703,315 - +0.9 1,703,345 - Pf1N1B4_1481 0.87 -1.1 1,703,345 - Pf1N1B4_1481 0.87 -2.1 1,703,446 + -1.9 1,703,446 + -1.1 1,703,447 - +0.6 1,703,481 + +1.2 1,703,481 + +0.0 1,703,481 + +0.9 1,703,482 - -2.3 1,703,482 - -1.1 1,703,482 - +1.2 1,703,488 + -0.2 1,703,489 - -1.1 1,703,526 - -1.1
Or see this region's nucleotide sequence