Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1277 and Pf1N1B4_1278 overlap by 4 nucleotides Pf1N1B4_1278 and Pf1N1B4_1279 are separated by 10 nucleotides Pf1N1B4_1279 and Pf1N1B4_1280 overlap by 4 nucleotides
Pf1N1B4_1277 - Flp pilus assembly protein CpaE, at 1,457,432 to 1,458,637
_1277
Pf1N1B4_1278 - Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily, at 1,458,634 to 1,459,914
_1278
Pf1N1B4_1279 - Flp pilus assembly protein TadB, at 1,459,925 to 1,460,809
_1279
Pf1N1B4_1280 - Type II/IV secretion system protein TadC, associated with Flp pilus assembly, at 1,460,806 to 1,461,696
_1280
Position (kb)
1458
1459
1460 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1457.763 kb on - strand, within Pf1N1B4_1277 at 1457.763 kb on - strand, within Pf1N1B4_1277 at 1457.873 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.247 kb on + strand, within Pf1N1B4_1277 at 1458.248 kb on - strand, within Pf1N1B4_1277 at 1458.281 kb on + strand, within Pf1N1B4_1277 at 1458.281 kb on + strand, within Pf1N1B4_1277 at 1458.282 kb on - strand, within Pf1N1B4_1277 at 1458.415 kb on + strand, within Pf1N1B4_1277 at 1458.474 kb on + strand, within Pf1N1B4_1277 at 1458.475 kb on - strand, within Pf1N1B4_1277 at 1458.721 kb on + strand at 1458.814 kb on + strand, within Pf1N1B4_1278 at 1458.814 kb on + strand, within Pf1N1B4_1278 at 1458.815 kb on - strand, within Pf1N1B4_1278 at 1458.815 kb on - strand, within Pf1N1B4_1278 at 1458.815 kb on - strand, within Pf1N1B4_1278 at 1458.815 kb on - strand, within Pf1N1B4_1278 at 1458.815 kb on - strand, within Pf1N1B4_1278 at 1459.252 kb on - strand, within Pf1N1B4_1278 at 1459.415 kb on + strand, within Pf1N1B4_1278 at 1459.416 kb on - strand, within Pf1N1B4_1278 at 1459.777 kb on - strand, within Pf1N1B4_1278 at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.803 kb on + strand at 1459.809 kb on - strand at 1459.912 kb on + strand at 1459.912 kb on + strand at 1459.991 kb on + strand at 1459.992 kb on - strand at 1460.103 kb on + strand, within Pf1N1B4_1279 at 1460.104 kb on - strand, within Pf1N1B4_1279 at 1460.246 kb on + strand, within Pf1N1B4_1279 at 1460.247 kb on - strand, within Pf1N1B4_1279 at 1460.247 kb on - strand, within Pf1N1B4_1279 at 1460.699 kb on + strand, within Pf1N1B4_1279 at 1460.699 kb on + strand, within Pf1N1B4_1279 at 1460.699 kb on + strand, within Pf1N1B4_1279 at 1460.699 kb on + strand, within Pf1N1B4_1279 at 1460.805 kb on + strand at 1460.806 kb on - strand at 1460.813 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 1,457,763 - Pf1N1B4_1277 0.27 -0.4 1,457,763 - Pf1N1B4_1277 0.27 +0.4 1,457,873 + Pf1N1B4_1277 0.37 -1.5 1,458,164 + Pf1N1B4_1277 0.61 -0.4 1,458,164 + Pf1N1B4_1277 0.61 -1.5 1,458,164 + Pf1N1B4_1277 0.61 -2.0 1,458,164 + Pf1N1B4_1277 0.61 -0.6 1,458,164 + Pf1N1B4_1277 0.61 -1.3 1,458,164 + Pf1N1B4_1277 0.61 -0.3 1,458,164 + Pf1N1B4_1277 0.61 -1.3 1,458,165 - Pf1N1B4_1277 0.61 +0.3 1,458,165 - Pf1N1B4_1277 0.61 -2.0 1,458,165 - Pf1N1B4_1277 0.61 -0.2 1,458,165 - Pf1N1B4_1277 0.61 +0.1 1,458,165 - Pf1N1B4_1277 0.61 +1.7 1,458,165 - Pf1N1B4_1277 0.61 +0.6 1,458,165 - Pf1N1B4_1277 0.61 -0.6 1,458,247 + Pf1N1B4_1277 0.68 -0.1 1,458,248 - Pf1N1B4_1277 0.68 -0.6 1,458,281 + Pf1N1B4_1277 0.70 +0.3 1,458,281 + Pf1N1B4_1277 0.70 -0.9 1,458,282 - Pf1N1B4_1277 0.70 -0.2 1,458,415 + Pf1N1B4_1277 0.82 -0.0 1,458,474 + Pf1N1B4_1277 0.86 +0.1 1,458,475 - Pf1N1B4_1277 0.86 -0.4 1,458,721 + -0.7 1,458,814 + Pf1N1B4_1278 0.14 -0.1 1,458,814 + Pf1N1B4_1278 0.14 -0.7 1,458,815 - Pf1N1B4_1278 0.14 +0.2 1,458,815 - Pf1N1B4_1278 0.14 -1.7 1,458,815 - Pf1N1B4_1278 0.14 -0.3 1,458,815 - Pf1N1B4_1278 0.14 -0.4 1,458,815 - Pf1N1B4_1278 0.14 -0.6 1,459,252 - Pf1N1B4_1278 0.48 -0.7 1,459,415 + Pf1N1B4_1278 0.61 -1.6 1,459,416 - Pf1N1B4_1278 0.61 +0.9 1,459,777 - Pf1N1B4_1278 0.89 -1.0 1,459,792 + -0.1 1,459,792 + +2.1 1,459,792 + -0.1 1,459,792 + +0.4 1,459,792 + +0.2 1,459,792 + -0.7 1,459,793 - +0.4 1,459,793 - -0.5 1,459,793 - +3.2 1,459,793 - +0.3 1,459,793 - +0.6 1,459,793 - -0.3 1,459,793 - -1.5 1,459,793 - +0.4 1,459,803 + +0.9 1,459,809 - -0.8 1,459,912 + +1.6 1,459,912 + -0.3 1,459,991 + +0.8 1,459,992 - -1.3 1,460,103 + Pf1N1B4_1279 0.20 -0.4 1,460,104 - Pf1N1B4_1279 0.20 -0.2 1,460,246 + Pf1N1B4_1279 0.36 +0.1 1,460,247 - Pf1N1B4_1279 0.36 +1.4 1,460,247 - Pf1N1B4_1279 0.36 +0.7 1,460,699 + Pf1N1B4_1279 0.87 -0.5 1,460,699 + Pf1N1B4_1279 0.87 +0.4 1,460,699 + Pf1N1B4_1279 0.87 -0.7 1,460,699 + Pf1N1B4_1279 0.87 +0.6 1,460,805 + -0.3 1,460,806 - +0.1 1,460,813 + +1.3
Or see this region's nucleotide sequence