Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06360 and MMP_RS06365 are separated by 30 nucleotides MMP_RS06365 and MMP_RS06370 are separated by 78 nucleotides MMP_RS06370 and MMP_RS06375 overlap by 4 nucleotides MMP_RS06375 and MMP_RS06380 are separated by 27 nucleotides
MMP_RS06360: MMP_RS06360 - UPF0280 family protein, at 1,222,830 to 1,223,573
_RS06360
MMP_RS06365: MMP_RS06365 - hypothetical protein, at 1,223,604 to 1,223,804
_RS06365
MMP_RS06370: MMP_RS06370 - biotin synthase BioB, at 1,223,883 to 1,224,866
_RS06370
MMP_RS06375: MMP_RS06375 - ATP-dependent sacrificial sulfur transferase LarE, at 1,224,863 to 1,225,639
_RS06375
MMP_RS06380: MMP_RS06380 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,225,667 to 1,226,809
_RS06380
Position (kb)
1223
1224
1225 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1222.926 kb on + strand at 1222.982 kb on - strand at 1223.426 kb on - strand at 1223.427 kb on + strand at 1223.577 kb on + strand at 1223.577 kb on + strand at 1223.577 kb on + strand at 1223.582 kb on - strand at 1223.583 kb on + strand at 1223.583 kb on + strand at 1223.584 kb on - strand at 1223.584 kb on - strand at 1223.585 kb on + strand at 1223.585 kb on + strand at 1223.587 kb on - strand at 1223.588 kb on + strand at 1223.588 kb on + strand at 1223.588 kb on + strand at 1223.588 kb on + strand at 1223.589 kb on - strand at 1223.589 kb on - strand at 1223.589 kb on - strand at 1223.589 kb on - strand at 1223.589 kb on - strand at 1223.590 kb on + strand at 1223.590 kb on + strand at 1223.590 kb on + strand at 1223.598 kb on - strand at 1223.599 kb on + strand at 1223.622 kb on + strand at 1223.627 kb on - strand, within MMP_RS06365 at 1223.667 kb on + strand, within MMP_RS06365 at 1223.834 kb on + strand at 1224.010 kb on + strand, within MMP_RS06370 at 1224.221 kb on + strand, within MMP_RS06370 at 1224.364 kb on - strand, within MMP_RS06370 at 1224.417 kb on + strand, within MMP_RS06370 at 1224.448 kb on - strand, within MMP_RS06370 at 1224.577 kb on - strand, within MMP_RS06370 at 1224.897 kb on + strand at 1224.933 kb on - strand at 1224.971 kb on + strand, within MMP_RS06375 at 1224.971 kb on + strand, within MMP_RS06375 at 1225.246 kb on + strand, within MMP_RS06375 at 1225.378 kb on - strand, within MMP_RS06375 at 1225.485 kb on + strand, within MMP_RS06375 at 1225.570 kb on + strand at 1225.582 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 1,222,926 + -4.5 1,222,982 - -3.8 1,223,426 - -3.1 1,223,427 + -2.8 1,223,577 + -0.6 1,223,577 + +0.1 1,223,577 + +0.1 1,223,582 - -0.2 1,223,583 + +0.1 1,223,583 + -0.4 1,223,584 - +0.1 1,223,584 - +1.7 1,223,585 + +0.1 1,223,585 + +0.2 1,223,587 - +0.2 1,223,588 + +0.2 1,223,588 + +1.9 1,223,588 + +0.1 1,223,588 + +0.0 1,223,589 - +0.3 1,223,589 - +0.0 1,223,589 - +0.0 1,223,589 - -0.3 1,223,589 - -0.4 1,223,590 + +0.2 1,223,590 + +0.2 1,223,590 + -0.3 1,223,598 - -0.1 1,223,599 + +0.7 1,223,622 + +0.6 1,223,627 - MMP_RS06365 0.11 -0.1 1,223,667 + MMP_RS06365 0.31 +0.1 1,223,834 + +0.3 1,224,010 + MMP_RS06370 0.13 -3.8 1,224,221 + MMP_RS06370 0.34 -1.2 1,224,364 - MMP_RS06370 0.49 -4.0 1,224,417 + MMP_RS06370 0.54 -4.0 1,224,448 - MMP_RS06370 0.57 -5.4 1,224,577 - MMP_RS06370 0.71 -2.3 1,224,897 + -0.6 1,224,933 - -0.6 1,224,971 + MMP_RS06375 0.14 -0.7 1,224,971 + MMP_RS06375 0.14 +0.1 1,225,246 + MMP_RS06375 0.49 +0.1 1,225,378 - MMP_RS06375 0.66 -0.6 1,225,485 + MMP_RS06375 0.80 -0.6 1,225,570 + -0.8 1,225,582 - -1.8
Or see this region's nucleotide sequence