Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03260 and MMP_RS03265 are separated by 19 nucleotides MMP_RS03265 and MMP_RS03270 are separated by 46 nucleotides MMP_RS03270 and MMP_RS03275 are separated by 64 nucleotides MMP_RS03275 and MMP_RS03280 are separated by 63 nucleotides
MMP_RS03260: MMP_RS03260 - methyl coenzyme M reductase system component A2, at 614,221 to 615,816
_RS03260
MMP_RS03265: MMP_RS03265 - sugar phosphate isomerase/epimerase, at 615,836 to 616,645
_RS03265
MMP_RS03270: MMP_RS03270 - ADP-ribosylglycohydrolase family protein, at 616,692 to 617,600
_RS03270
MMP_RS03275: MMP_RS03275 - TIGR00266 family protein, at 617,665 to 618,333
_RS03275
MMP_RS03280: MMP_RS03280 - pyridoxamine 5'-phosphate oxidase family protein, at 618,397 to 618,798
_RS03280
Position (kb) 616
617
618 Strain fitness (log2 ratio) -2
-1
0
1 at 615.871 kb on - strand at 615.875 kb on - strand at 615.876 kb on + strand at 615.880 kb on - strand at 615.880 kb on - strand at 615.880 kb on - strand at 615.884 kb on - strand at 615.913 kb on - strand at 615.915 kb on - strand at 615.915 kb on - strand at 615.916 kb on + strand at 615.916 kb on + strand at 615.916 kb on + strand at 615.917 kb on - strand, within MMP_RS03265 at 615.930 kb on + strand, within MMP_RS03265 at 615.958 kb on - strand, within MMP_RS03265 at 615.981 kb on + strand, within MMP_RS03265 at 616.193 kb on - strand, within MMP_RS03265 at 616.279 kb on - strand, within MMP_RS03265 at 616.280 kb on + strand, within MMP_RS03265 at 616.409 kb on + strand, within MMP_RS03265 at 616.409 kb on + strand, within MMP_RS03265 at 616.454 kb on - strand, within MMP_RS03265 at 616.454 kb on - strand, within MMP_RS03265 at 616.454 kb on - strand, within MMP_RS03265 at 616.455 kb on + strand, within MMP_RS03265 at 616.455 kb on + strand, within MMP_RS03265 at 616.455 kb on + strand, within MMP_RS03265 at 616.456 kb on - strand, within MMP_RS03265 at 616.457 kb on + strand, within MMP_RS03265 at 616.460 kb on - strand, within MMP_RS03265 at 616.460 kb on - strand, within MMP_RS03265 at 616.461 kb on + strand, within MMP_RS03265 at 616.466 kb on - strand, within MMP_RS03265 at 616.466 kb on - strand, within MMP_RS03265 at 616.496 kb on - strand, within MMP_RS03265 at 616.496 kb on - strand, within MMP_RS03265 at 616.497 kb on + strand, within MMP_RS03265 at 616.506 kb on + strand, within MMP_RS03265 at 616.581 kb on + strand at 616.581 kb on + strand at 616.582 kb on - strand at 616.582 kb on - strand at 616.583 kb on + strand at 616.587 kb on + strand at 616.606 kb on + strand at 616.656 kb on - strand at 616.656 kb on - strand at 616.672 kb on - strand at 616.962 kb on - strand, within MMP_RS03270 at 616.970 kb on - strand, within MMP_RS03270 at 616.970 kb on - strand, within MMP_RS03270 at 617.033 kb on - strand, within MMP_RS03270 at 617.127 kb on + strand, within MMP_RS03270 at 617.434 kb on - strand, within MMP_RS03270 at 617.472 kb on - strand, within MMP_RS03270 at 617.650 kb on + strand at 617.783 kb on - strand, within MMP_RS03275 at 617.784 kb on + strand, within MMP_RS03275 at 617.853 kb on + strand, within MMP_RS03275 at 617.913 kb on - strand, within MMP_RS03275 at 617.915 kb on - strand, within MMP_RS03275 at 618.232 kb on - strand, within MMP_RS03275 at 618.233 kb on + strand, within MMP_RS03275 at 618.308 kb on + strand at 618.395 kb on + strand at 618.395 kb on + strand at 618.443 kb on - strand, within MMP_RS03280 at 618.482 kb on - strand, within MMP_RS03280 at 618.524 kb on - strand, within MMP_RS03280 at 618.579 kb on + strand, within MMP_RS03280 at 618.579 kb on + strand, within MMP_RS03280
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 615,871 - +0.2 615,875 - +0.2 615,876 + +0.6 615,880 - +0.5 615,880 - -0.3 615,880 - +0.4 615,884 - -0.2 615,913 - +0.9 615,915 - +0.1 615,915 - +0.6 615,916 + -0.8 615,916 + +1.2 615,916 + -0.2 615,917 - MMP_RS03265 0.10 -0.2 615,930 + MMP_RS03265 0.12 +0.4 615,958 - MMP_RS03265 0.15 -0.1 615,981 + MMP_RS03265 0.18 +0.6 616,193 - MMP_RS03265 0.44 +0.4 616,279 - MMP_RS03265 0.55 -1.0 616,280 + MMP_RS03265 0.55 -0.2 616,409 + MMP_RS03265 0.71 -0.8 616,409 + MMP_RS03265 0.71 -0.2 616,454 - MMP_RS03265 0.76 +0.1 616,454 - MMP_RS03265 0.76 +0.8 616,454 - MMP_RS03265 0.76 -0.0 616,455 + MMP_RS03265 0.76 +0.2 616,455 + MMP_RS03265 0.76 +0.3 616,455 + MMP_RS03265 0.76 +0.6 616,456 - MMP_RS03265 0.77 -0.0 616,457 + MMP_RS03265 0.77 +0.5 616,460 - MMP_RS03265 0.77 -0.1 616,460 - MMP_RS03265 0.77 +0.1 616,461 + MMP_RS03265 0.77 -0.4 616,466 - MMP_RS03265 0.78 +0.2 616,466 - MMP_RS03265 0.78 +0.5 616,496 - MMP_RS03265 0.81 -0.2 616,496 - MMP_RS03265 0.81 -0.2 616,497 + MMP_RS03265 0.82 +0.6 616,506 + MMP_RS03265 0.83 +0.2 616,581 + -0.0 616,581 + -0.1 616,582 - +0.6 616,582 - +0.2 616,583 + -0.1 616,587 + +0.1 616,606 + +0.2 616,656 - -0.6 616,656 - -0.5 616,672 - -0.5 616,962 - MMP_RS03270 0.30 -2.1 616,970 - MMP_RS03270 0.31 -2.2 616,970 - MMP_RS03270 0.31 -2.5 617,033 - MMP_RS03270 0.38 -2.3 617,127 + MMP_RS03270 0.48 -2.2 617,434 - MMP_RS03270 0.82 -2.0 617,472 - MMP_RS03270 0.86 -2.0 617,650 + +0.3 617,783 - MMP_RS03275 0.18 +1.3 617,784 + MMP_RS03275 0.18 -0.1 617,853 + MMP_RS03275 0.28 +0.2 617,913 - MMP_RS03275 0.37 +0.6 617,915 - MMP_RS03275 0.37 +0.2 618,232 - MMP_RS03275 0.85 -0.0 618,233 + MMP_RS03275 0.85 -0.2 618,308 + -0.5 618,395 + -0.4 618,395 + +0.9 618,443 - MMP_RS03280 0.11 -0.0 618,482 - MMP_RS03280 0.21 +0.1 618,524 - MMP_RS03280 0.32 +0.0 618,579 + MMP_RS03280 0.45 +0.6 618,579 + MMP_RS03280 0.45 -0.2
Or see this region's nucleotide sequence