Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03005 and MMP_RS03010 are separated by 73 nucleotides MMP_RS03010 and MMP_RS03015 are separated by 8 nucleotides MMP_RS03015 and MMP_RS03020 are separated by 131 nucleotides MMP_RS03020 and MMP_RS03025 are separated by 110 nucleotides
MMP_RS03005: MMP_RS03005 - S-layer protein, at 568,527 to 570,113
_RS03005
MMP_RS03010: MMP_RS03010 - cupin domain-containing protein, at 570,187 to 570,687
_RS03010
MMP_RS03015: MMP_RS03015 - GTP 3' 8-cyclase MoaA, at 570,696 to 571,589
_RS03015
MMP_RS03020: MMP_RS03020 - peptidylprolyl isomerase, at 571,721 to 572,407
_RS03020
MMP_RS03025: MMP_RS03025 - molybdenum cofactor guanylyltransferase, at 572,518 to 573,162
_RS03025
Position (kb) 570
571
572 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 569.731 kb on - strand, within MMP_RS03005 at 569.731 kb on - strand, within MMP_RS03005 at 570.001 kb on + strand at 570.060 kb on - strand at 570.061 kb on + strand at 570.064 kb on + strand at 570.064 kb on + strand at 570.068 kb on - strand at 570.078 kb on - strand at 570.079 kb on + strand at 570.083 kb on - strand at 570.083 kb on - strand at 570.113 kb on - strand at 570.135 kb on - strand at 570.139 kb on + strand at 570.139 kb on + strand at 570.142 kb on + strand at 570.164 kb on - strand at 570.361 kb on + strand, within MMP_RS03010 at 570.410 kb on + strand, within MMP_RS03010 at 570.414 kb on - strand, within MMP_RS03010 at 570.415 kb on + strand, within MMP_RS03010 at 570.416 kb on - strand, within MMP_RS03010 at 570.436 kb on + strand, within MMP_RS03010 at 570.439 kb on + strand, within MMP_RS03010 at 570.445 kb on + strand, within MMP_RS03010 at 570.448 kb on - strand, within MMP_RS03010 at 570.449 kb on + strand, within MMP_RS03010 at 570.449 kb on + strand, within MMP_RS03010 at 570.468 kb on + strand, within MMP_RS03010 at 570.524 kb on + strand, within MMP_RS03010 at 570.534 kb on + strand, within MMP_RS03010 at 570.544 kb on + strand, within MMP_RS03010 at 570.548 kb on - strand, within MMP_RS03010 at 570.567 kb on + strand, within MMP_RS03010 at 570.567 kb on + strand, within MMP_RS03010 at 570.577 kb on - strand, within MMP_RS03010 at 570.820 kb on + strand, within MMP_RS03015 at 570.836 kb on - strand, within MMP_RS03015 at 570.915 kb on + strand, within MMP_RS03015 at 570.950 kb on - strand, within MMP_RS03015 at 570.951 kb on + strand, within MMP_RS03015 at 570.954 kb on - strand, within MMP_RS03015 at 570.954 kb on - strand, within MMP_RS03015 at 570.954 kb on - strand, within MMP_RS03015 at 570.955 kb on + strand, within MMP_RS03015 at 570.955 kb on + strand, within MMP_RS03015 at 570.955 kb on + strand, within MMP_RS03015 at 571.002 kb on - strand, within MMP_RS03015 at 571.003 kb on + strand, within MMP_RS03015 at 571.239 kb on + strand, within MMP_RS03015 at 571.302 kb on - strand, within MMP_RS03015 at 571.354 kb on - strand, within MMP_RS03015 at 571.668 kb on + strand at 571.709 kb on - strand at 571.723 kb on + strand at 571.863 kb on + strand, within MMP_RS03020 at 571.863 kb on + strand, within MMP_RS03020 at 571.863 kb on + strand, within MMP_RS03020 at 571.868 kb on - strand, within MMP_RS03020 at 571.941 kb on + strand, within MMP_RS03020 at 571.964 kb on - strand, within MMP_RS03020 at 572.054 kb on - strand, within MMP_RS03020 at 572.430 kb on - strand at 572.461 kb on - strand at 572.461 kb on - strand at 572.479 kb on - strand at 572.479 kb on - strand at 572.499 kb on + strand at 572.518 kb on + strand at 572.532 kb on - strand at 572.533 kb on + strand at 572.538 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 569,731 - MMP_RS03005 0.76 +0.0 569,731 - MMP_RS03005 0.76 +0.3 570,001 + -0.0 570,060 - -0.7 570,061 + +0.8 570,064 + -0.1 570,064 + -0.1 570,068 - +0.1 570,078 - -0.3 570,079 + +1.0 570,083 - +0.3 570,083 - -0.1 570,113 - +0.2 570,135 - -0.1 570,139 + -0.4 570,139 + +0.2 570,142 + -0.1 570,164 - +1.3 570,361 + MMP_RS03010 0.35 +0.0 570,410 + MMP_RS03010 0.45 -0.2 570,414 - MMP_RS03010 0.45 -0.2 570,415 + MMP_RS03010 0.46 -0.5 570,416 - MMP_RS03010 0.46 -0.1 570,436 + MMP_RS03010 0.50 -0.4 570,439 + MMP_RS03010 0.50 -0.7 570,445 + MMP_RS03010 0.51 +0.1 570,448 - MMP_RS03010 0.52 +0.5 570,449 + MMP_RS03010 0.52 +0.0 570,449 + MMP_RS03010 0.52 -0.1 570,468 + MMP_RS03010 0.56 -0.2 570,524 + MMP_RS03010 0.67 +0.4 570,534 + MMP_RS03010 0.69 +0.6 570,544 + MMP_RS03010 0.71 -0.3 570,548 - MMP_RS03010 0.72 +0.4 570,567 + MMP_RS03010 0.76 -0.3 570,567 + MMP_RS03010 0.76 +0.7 570,577 - MMP_RS03010 0.78 -0.5 570,820 + MMP_RS03015 0.14 -4.3 570,836 - MMP_RS03015 0.16 -4.9 570,915 + MMP_RS03015 0.24 -4.9 570,950 - MMP_RS03015 0.28 -3.1 570,951 + MMP_RS03015 0.29 -5.2 570,954 - MMP_RS03015 0.29 -3.8 570,954 - MMP_RS03015 0.29 -4.6 570,954 - MMP_RS03015 0.29 -5.0 570,955 + MMP_RS03015 0.29 -4.4 570,955 + MMP_RS03015 0.29 -5.6 570,955 + MMP_RS03015 0.29 -6.5 571,002 - MMP_RS03015 0.34 -3.9 571,003 + MMP_RS03015 0.34 -4.6 571,239 + MMP_RS03015 0.61 -4.1 571,302 - MMP_RS03015 0.68 -5.3 571,354 - MMP_RS03015 0.74 -4.7 571,668 + +0.3 571,709 - -0.1 571,723 + -1.2 571,863 + MMP_RS03020 0.21 -1.5 571,863 + MMP_RS03020 0.21 -1.5 571,863 + MMP_RS03020 0.21 -2.3 571,868 - MMP_RS03020 0.21 -0.9 571,941 + MMP_RS03020 0.32 -1.1 571,964 - MMP_RS03020 0.35 +0.0 572,054 - MMP_RS03020 0.48 -1.1 572,430 - -1.0 572,461 - -1.1 572,461 - -1.2 572,479 - -1.5 572,479 - -2.0 572,499 + -1.6 572,518 + -4.5 572,532 - -4.3 572,533 + -5.0 572,538 + -2.8
Or see this region's nucleotide sequence