Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01565 and MMP_RS01570 overlap by 4 nucleotides MMP_RS01570 and MMP_RS01575 are separated by 11 nucleotides MMP_RS01575 and MMP_RS01580 are separated by 158 nucleotides
MMP_RS01565: MMP_RS01565 - AIR synthase related protein, at 291,659 to 293,002
_RS01565
MMP_RS01570: MMP_RS01570 - hypothetical protein, at 292,999 to 293,184
_RS01570
MMP_RS01575: MMP_RS01575 - aldolase, at 293,196 to 294,122
_RS01575
MMP_RS01580: MMP_RS01580 - PQQ-binding-like beta-propeller repeat protein, at 294,281 to 295,129
_RS01580
Position (kb) 293
294
295 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 292.597 kb on - strand at 292.620 kb on - strand at 293.157 kb on - strand, within MMP_RS01570 at 293.187 kb on - strand at 293.198 kb on + strand at 293.217 kb on - strand at 293.219 kb on - strand at 293.220 kb on + strand at 293.413 kb on + strand, within MMP_RS01575 at 293.420 kb on - strand, within MMP_RS01575 at 293.438 kb on - strand, within MMP_RS01575 at 293.487 kb on - strand, within MMP_RS01575 at 293.682 kb on - strand, within MMP_RS01575 at 293.682 kb on - strand, within MMP_RS01575 at 293.838 kb on + strand, within MMP_RS01575 at 294.029 kb on - strand, within MMP_RS01575 at 294.137 kb on - strand at 294.145 kb on + strand at 294.152 kb on - strand at 294.153 kb on + strand at 294.164 kb on - strand at 294.164 kb on - strand at 294.172 kb on - strand at 294.195 kb on + strand at 294.224 kb on + strand at 294.224 kb on + strand at 294.225 kb on - strand at 294.226 kb on + strand at 294.226 kb on + strand at 294.226 kb on + strand at 294.226 kb on + strand at 294.227 kb on - strand at 294.228 kb on + strand at 294.241 kb on + strand at 294.241 kb on + strand at 294.241 kb on + strand at 294.244 kb on - strand at 294.244 kb on - strand at 294.245 kb on + strand at 294.245 kb on + strand at 294.245 kb on + strand at 294.245 kb on + strand at 294.245 kb on + strand at 294.338 kb on + strand at 294.338 kb on + strand at 294.377 kb on + strand, within MMP_RS01580 at 294.377 kb on + strand, within MMP_RS01580 at 294.398 kb on + strand, within MMP_RS01580 at 294.398 kb on + strand, within MMP_RS01580 at 294.459 kb on - strand, within MMP_RS01580 at 294.470 kb on + strand, within MMP_RS01580 at 294.515 kb on + strand, within MMP_RS01580 at 294.637 kb on + strand, within MMP_RS01580 at 294.752 kb on - strand, within MMP_RS01580 at 294.753 kb on + strand, within MMP_RS01580 at 294.870 kb on - strand, within MMP_RS01580 at 294.966 kb on + strand, within MMP_RS01580 at 295.055 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 292,597 - -4.2 292,620 - +0.5 293,157 - MMP_RS01570 0.85 +0.6 293,187 - +0.5 293,198 + +0.6 293,217 - -1.8 293,219 - -0.5 293,220 + +0.2 293,413 + MMP_RS01575 0.23 -3.5 293,420 - MMP_RS01575 0.24 -2.4 293,438 - MMP_RS01575 0.26 -3.0 293,487 - MMP_RS01575 0.31 -1.0 293,682 - MMP_RS01575 0.52 -4.1 293,682 - MMP_RS01575 0.52 -3.3 293,838 + MMP_RS01575 0.69 -2.4 294,029 - MMP_RS01575 0.90 -2.9 294,137 - -3.6 294,145 + -0.6 294,152 - -0.3 294,153 + -2.9 294,164 - +0.7 294,164 - -0.3 294,172 - -0.9 294,195 + +0.3 294,224 + +0.2 294,224 + +0.5 294,225 - -0.3 294,226 + -0.4 294,226 + +0.1 294,226 + +0.1 294,226 + -0.5 294,227 - +0.1 294,228 + -2.4 294,241 + +0.6 294,241 + +0.4 294,241 + -0.3 294,244 - +0.2 294,244 - -0.1 294,245 + -0.0 294,245 + +1.2 294,245 + +0.3 294,245 + +0.1 294,245 + +0.5 294,338 + +0.1 294,338 + +0.3 294,377 + MMP_RS01580 0.11 +0.2 294,377 + MMP_RS01580 0.11 +0.3 294,398 + MMP_RS01580 0.14 +0.0 294,398 + MMP_RS01580 0.14 +0.4 294,459 - MMP_RS01580 0.21 -0.3 294,470 + MMP_RS01580 0.22 -0.4 294,515 + MMP_RS01580 0.28 -0.2 294,637 + MMP_RS01580 0.42 +0.4 294,752 - MMP_RS01580 0.55 -0.3 294,753 + MMP_RS01580 0.56 -0.8 294,870 - MMP_RS01580 0.69 -0.3 294,966 + MMP_RS01580 0.81 -0.6 295,055 + +0.3
Or see this region's nucleotide sequence