Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01400 and MMP_RS01405 are separated by 13 nucleotides MMP_RS01405 and MMP_RS01410 are separated by 178 nucleotides MMP_RS01410 and MMP_RS01415 overlap by 4 nucleotides MMP_RS01415 and MMP_RS08935 are separated by 331 nucleotides
MMP_RS01400: MMP_RS01400 - 50S ribosomal protein L10, at 260,003 to 261,010
_RS01400
MMP_RS01405: MMP_RS01405 - 50S ribosomal protein L1, at 261,024 to 261,665
_RS01405
MMP_RS01410: MMP_RS01410 - DNA-directed RNA polymerase subunit L, at 261,844 to 262,134
_RS01410
MMP_RS01415: MMP_RS01415 - DUF2067 domain-containing protein, at 262,131 to 262,748
_RS01415
MMP_RS08935: MMP_RS08935 - hypothetical protein, at 263,080 to 263,229
_RS08935
Position (kb) 261
262
263 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 260.986 kb on - strand at 261.114 kb on - strand at 261.403 kb on + strand at 261.724 kb on - strand at 261.833 kb on - strand at 262.128 kb on + strand at 262.170 kb on - strand at 262.212 kb on - strand, within MMP_RS01415 at 262.333 kb on - strand, within MMP_RS01415 at 262.353 kb on - strand, within MMP_RS01415 at 262.419 kb on + strand, within MMP_RS01415 at 262.625 kb on + strand, within MMP_RS01415 at 262.682 kb on - strand, within MMP_RS01415 at 262.682 kb on - strand, within MMP_RS01415 at 262.682 kb on - strand, within MMP_RS01415 at 262.729 kb on + strand at 262.734 kb on - strand at 262.748 kb on - strand at 262.762 kb on - strand at 262.789 kb on - strand at 262.790 kb on + strand at 262.793 kb on - strand at 262.796 kb on + strand at 262.824 kb on - strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.828 kb on - strand at 262.828 kb on - strand at 262.834 kb on - strand at 262.835 kb on + strand at 262.839 kb on + strand at 262.849 kb on - strand at 262.926 kb on - strand at 262.927 kb on + strand at 263.020 kb on - strand at 263.020 kb on - strand at 263.069 kb on - strand at 263.072 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 260,986 - -1.8 261,114 - -2.5 261,403 + -2.6 261,724 - -2.4 261,833 - -1.3 262,128 + -3.5 262,170 - -4.1 262,212 - MMP_RS01415 0.13 -3.2 262,333 - MMP_RS01415 0.33 -2.0 262,353 - MMP_RS01415 0.36 -3.0 262,419 + MMP_RS01415 0.47 -0.6 262,625 + MMP_RS01415 0.80 -3.1 262,682 - MMP_RS01415 0.89 -1.8 262,682 - MMP_RS01415 0.89 -1.3 262,682 - MMP_RS01415 0.89 -2.5 262,729 + -4.5 262,734 - -2.4 262,748 - -1.4 262,762 - +0.0 262,789 - -1.2 262,790 + -4.3 262,793 - -1.4 262,796 + -3.3 262,824 - -2.4 262,825 + -2.8 262,825 + -3.6 262,828 - -2.5 262,828 - -1.8 262,834 - -0.3 262,835 + -0.7 262,839 + -0.0 262,849 - +0.4 262,926 - +0.2 262,927 + +0.9 263,020 - +0.9 263,020 - +0.2 263,069 - -0.3 263,072 - -0.4
Or see this region's nucleotide sequence