Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00105 and MMP_RS00110 are separated by 14 nucleotides MMP_RS00110 and MMP_RS00115 are separated by 120 nucleotides MMP_RS00115 and MMP_RS00120 are separated by 72 nucleotides MMP_RS00120 and MMP_RS00125 are separated by 29 nucleotides
MMP_RS00105: MMP_RS00105 - hypothetical protein, at 25,243 to 26,046
_RS00105
MMP_RS00110: MMP_RS00110 - hypothetical protein, at 26,061 to 26,552
_RS00110
MMP_RS00115: MMP_RS00115 - radical SAM protein, at 26,673 to 27,725
_RS00115
MMP_RS00120: MMP_RS00120 - LysR family transcriptional regulator, at 27,798 to 28,640
_RS00120
MMP_RS00125: MMP_RS00125 - DUF2798 domain-containing protein, at 28,670 to 28,897
_RS00125
Position (kb) 26
27
28 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 25.741 kb on + strand, within MMP_RS00105 at 25.784 kb on + strand, within MMP_RS00105 at 25.789 kb on - strand, within MMP_RS00105 at 25.789 kb on - strand, within MMP_RS00105 at 25.789 kb on - strand, within MMP_RS00105 at 25.793 kb on + strand, within MMP_RS00105 at 25.867 kb on + strand, within MMP_RS00105 at 25.899 kb on - strand, within MMP_RS00105 at 25.904 kb on - strand, within MMP_RS00105 at 25.905 kb on + strand, within MMP_RS00105 at 25.905 kb on + strand, within MMP_RS00105 at 26.026 kb on - strand at 26.028 kb on - strand at 26.049 kb on - strand at 26.099 kb on - strand at 26.109 kb on - strand at 26.110 kb on + strand at 26.122 kb on - strand, within MMP_RS00110 at 26.123 kb on + strand, within MMP_RS00110 at 26.355 kb on - strand, within MMP_RS00110 at 26.368 kb on - strand, within MMP_RS00110 at 26.412 kb on - strand, within MMP_RS00110 at 26.413 kb on + strand, within MMP_RS00110 at 26.564 kb on - strand at 26.587 kb on - strand at 26.587 kb on - strand at 26.587 kb on - strand at 26.600 kb on - strand at 26.600 kb on - strand at 26.600 kb on - strand at 26.600 kb on - strand at 26.600 kb on - strand at 26.600 kb on - strand at 26.601 kb on + strand at 26.601 kb on + strand at 26.601 kb on + strand at 26.601 kb on + strand at 26.601 kb on + strand at 26.601 kb on + strand at 26.602 kb on - strand at 26.602 kb on - strand at 26.602 kb on - strand at 26.602 kb on - strand at 26.602 kb on - strand at 26.602 kb on - strand at 26.603 kb on + strand at 26.603 kb on + strand at 26.603 kb on + strand at 26.603 kb on + strand at 26.603 kb on + strand at 26.604 kb on - strand at 26.604 kb on - strand at 26.604 kb on - strand at 26.604 kb on - strand at 26.604 kb on - strand at 26.604 kb on - strand at 26.605 kb on + strand at 26.605 kb on + strand at 26.605 kb on + strand at 26.605 kb on + strand at 26.605 kb on + strand at 26.605 kb on + strand at 26.605 kb on + strand at 26.607 kb on + strand at 26.635 kb on - strand at 26.654 kb on + strand at 26.654 kb on + strand at 26.660 kb on - strand at 26.660 kb on - strand at 26.660 kb on - strand at 26.661 kb on + strand at 26.665 kb on + strand at 26.665 kb on + strand at 26.665 kb on + strand at 26.666 kb on - strand at 26.667 kb on + strand at 26.670 kb on + strand at 26.670 kb on + strand at 26.670 kb on + strand at 26.671 kb on - strand at 26.672 kb on + strand at 26.672 kb on + strand at 26.702 kb on - strand at 26.750 kb on - strand at 26.751 kb on + strand at 26.751 kb on + strand at 26.774 kb on - strand at 26.774 kb on - strand at 26.794 kb on - strand, within MMP_RS00115 at 26.794 kb on - strand, within MMP_RS00115 at 26.829 kb on - strand, within MMP_RS00115 at 26.852 kb on - strand, within MMP_RS00115 at 26.855 kb on + strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.915 kb on + strand, within MMP_RS00115 at 26.939 kb on - strand, within MMP_RS00115 at 26.940 kb on + strand, within MMP_RS00115 at 26.944 kb on + strand, within MMP_RS00115 at 26.981 kb on - strand, within MMP_RS00115 at 26.987 kb on - strand, within MMP_RS00115 at 27.057 kb on + strand, within MMP_RS00115 at 27.209 kb on - strand, within MMP_RS00115 at 27.387 kb on - strand, within MMP_RS00115 at 27.413 kb on + strand, within MMP_RS00115 at 27.473 kb on - strand, within MMP_RS00115 at 27.492 kb on - strand, within MMP_RS00115 at 27.493 kb on + strand, within MMP_RS00115 at 27.596 kb on - strand, within MMP_RS00115 at 27.614 kb on - strand, within MMP_RS00115 at 27.650 kb on - strand at 27.725 kb on - strand at 27.728 kb on - strand at 27.739 kb on + strand at 27.764 kb on - strand at 27.765 kb on + strand at 27.823 kb on - strand at 27.824 kb on + strand at 27.840 kb on + strand at 27.842 kb on + strand at 27.846 kb on - strand at 27.846 kb on - strand at 27.847 kb on + strand at 27.847 kb on + strand at 27.848 kb on - strand at 28.059 kb on - strand, within MMP_RS00120 at 28.060 kb on + strand, within MMP_RS00120 at 28.071 kb on - strand, within MMP_RS00120 at 28.115 kb on - strand, within MMP_RS00120 at 28.118 kb on + strand, within MMP_RS00120 at 28.332 kb on - strand, within MMP_RS00120 at 28.609 kb on - strand at 28.657 kb on + strand at 28.659 kb on - strand at 28.660 kb on + strand at 28.683 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 25,741 + MMP_RS00105 0.62 -0.2 25,784 + MMP_RS00105 0.67 +0.6 25,789 - MMP_RS00105 0.68 -0.1 25,789 - MMP_RS00105 0.68 -0.1 25,789 - MMP_RS00105 0.68 +0.1 25,793 + MMP_RS00105 0.68 -0.6 25,867 + MMP_RS00105 0.78 -0.1 25,899 - MMP_RS00105 0.82 -0.2 25,904 - MMP_RS00105 0.82 +1.0 25,905 + MMP_RS00105 0.82 -0.2 25,905 + MMP_RS00105 0.82 +0.7 26,026 - +0.4 26,028 - +0.1 26,049 - +0.1 26,099 - -2.8 26,109 - -3.7 26,110 + -1.5 26,122 - MMP_RS00110 0.12 -3.3 26,123 + MMP_RS00110 0.13 -4.3 26,355 - MMP_RS00110 0.60 -3.5 26,368 - MMP_RS00110 0.62 -2.4 26,412 - MMP_RS00110 0.71 -2.3 26,413 + MMP_RS00110 0.72 -5.2 26,564 - -3.3 26,587 - -0.3 26,587 - -0.4 26,587 - -0.2 26,600 - -0.1 26,600 - -0.2 26,600 - +0.0 26,600 - -2.2 26,600 - -0.3 26,600 - +0.1 26,601 + -3.8 26,601 + -1.9 26,601 + -3.0 26,601 + -2.4 26,601 + -1.8 26,601 + -2.6 26,602 - +0.1 26,602 - -0.2 26,602 - -0.0 26,602 - -1.4 26,602 - -0.0 26,602 - -0.1 26,603 + -1.0 26,603 + -2.4 26,603 + -1.5 26,603 + -1.7 26,603 + -1.9 26,604 - -1.0 26,604 - +0.6 26,604 - +0.0 26,604 - +0.3 26,604 - -0.1 26,604 - +0.2 26,605 + -0.2 26,605 + -0.0 26,605 + +0.2 26,605 + -0.2 26,605 + +0.1 26,605 + +0.1 26,605 + +0.1 26,607 + +0.1 26,635 - -0.1 26,654 + +0.5 26,654 + +0.4 26,660 - -0.5 26,660 - -0.3 26,660 - +1.0 26,661 + +0.2 26,665 + +0.2 26,665 + +0.3 26,665 + +0.1 26,666 - +0.3 26,667 + +0.3 26,670 + -0.1 26,670 + +0.1 26,670 + -0.2 26,671 - -0.7 26,672 + -0.0 26,672 + +0.2 26,702 - +0.1 26,750 - -1.7 26,751 + -3.5 26,751 + -1.3 26,774 - -3.2 26,774 - -3.8 26,794 - MMP_RS00115 0.11 -3.5 26,794 - MMP_RS00115 0.11 -3.7 26,829 - MMP_RS00115 0.15 -4.6 26,852 - MMP_RS00115 0.17 -2.1 26,855 + MMP_RS00115 0.17 -4.5 26,856 - MMP_RS00115 0.17 -2.9 26,856 - MMP_RS00115 0.17 -3.4 26,856 - MMP_RS00115 0.17 -2.6 26,856 - MMP_RS00115 0.17 -3.7 26,856 - MMP_RS00115 0.17 -4.5 26,857 + MMP_RS00115 0.17 -2.2 26,857 + MMP_RS00115 0.17 -2.6 26,857 + MMP_RS00115 0.17 -3.2 26,857 + MMP_RS00115 0.17 -2.7 26,915 + MMP_RS00115 0.23 -2.6 26,939 - MMP_RS00115 0.25 -2.7 26,940 + MMP_RS00115 0.25 -2.7 26,944 + MMP_RS00115 0.26 -3.2 26,981 - MMP_RS00115 0.29 -1.9 26,987 - MMP_RS00115 0.30 -1.1 27,057 + MMP_RS00115 0.36 -3.1 27,209 - MMP_RS00115 0.51 -0.9 27,387 - MMP_RS00115 0.68 -3.7 27,413 + MMP_RS00115 0.70 -2.3 27,473 - MMP_RS00115 0.76 -1.0 27,492 - MMP_RS00115 0.78 -2.8 27,493 + MMP_RS00115 0.78 -4.4 27,596 - MMP_RS00115 0.88 -1.5 27,614 - MMP_RS00115 0.89 -4.7 27,650 - -0.4 27,725 - -3.0 27,728 - -3.1 27,739 + -3.0 27,764 - -0.2 27,765 + -2.1 27,823 - +0.1 27,824 + -0.9 27,840 + -2.3 27,842 + -1.1 27,846 - -0.7 27,846 - -0.4 27,847 + +0.2 27,847 + -0.7 27,848 - -1.2 28,059 - MMP_RS00120 0.31 -0.3 28,060 + MMP_RS00120 0.31 -1.3 28,071 - MMP_RS00120 0.32 -0.5 28,115 - MMP_RS00120 0.38 -0.2 28,118 + MMP_RS00120 0.38 -0.5 28,332 - MMP_RS00120 0.63 -0.1 28,609 - +0.0 28,657 + +0.3 28,659 - +0.8 28,660 + -0.1 28,683 + -0.2
Or see this region's nucleotide sequence