Experiment: D,L-Malic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS13270 and HSERO_RS13275 are separated by 143 nucleotides HSERO_RS13275 and HSERO_RS13280 overlap by 4 nucleotides HSERO_RS13280 and HSERO_RS13285 overlap by 4 nucleotides
HSERO_RS13270: HSERO_RS13270 - bifunctional precorrin-3 methyltransferase/precorrin-6x reductase oxidoreductase, at 3,023,458 to 3,025,017
_RS13270
HSERO_RS13275: HSERO_RS13275 - cobalamin biosynthesis protein CbiG, at 3,025,161 to 3,025,964
_RS13275
HSERO_RS13280: HSERO_RS13280 - precorrin-4 C11-methyltransferase, at 3,025,961 to 3,026,737
_RS13280
HSERO_RS13285: HSERO_RS13285 - precorrin-2 C20-methyltransferase, at 3,026,734 to 3,027,492
_RS13285
Position (kb)
3025
3026 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3024.276 kb on + strand, within HSERO_RS13270 at 3024.284 kb on - strand, within HSERO_RS13270 at 3024.308 kb on + strand, within HSERO_RS13270 at 3024.452 kb on + strand, within HSERO_RS13270 at 3024.540 kb on - strand at 3024.974 kb on + strand at 3025.150 kb on - strand at 3025.397 kb on - strand, within HSERO_RS13275 at 3025.834 kb on - strand, within HSERO_RS13275 at 3025.931 kb on + strand at 3026.019 kb on - strand at 3026.078 kb on + strand, within HSERO_RS13280 at 3026.092 kb on - strand, within HSERO_RS13280 at 3026.461 kb on - strand, within HSERO_RS13280 at 3026.524 kb on + strand, within HSERO_RS13280
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Malic Acid (C) remove 3,024,276 + HSERO_RS13270 0.52 -1.0 3,024,284 - HSERO_RS13270 0.53 -2.0 3,024,308 + HSERO_RS13270 0.54 -0.6 3,024,452 + HSERO_RS13270 0.64 -1.7 3,024,540 - -1.8 3,024,974 + -1.5 3,025,150 - +0.4 3,025,397 - HSERO_RS13275 0.29 -2.1 3,025,834 - HSERO_RS13275 0.84 -0.6 3,025,931 + -0.8 3,026,019 - -0.6 3,026,078 + HSERO_RS13280 0.15 -1.4 3,026,092 - HSERO_RS13280 0.17 -2.3 3,026,461 - HSERO_RS13280 0.64 -2.6 3,026,524 + HSERO_RS13280 0.72 -2.9
Or see this region's nucleotide sequence