Experiment: D,L-Malic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02115 and HSERO_RS02120 are separated by 617 nucleotides HSERO_RS02120 and HSERO_RS02125 are separated by 5 nucleotides HSERO_RS02125 and HSERO_RS02130 are separated by 38 nucleotides
HSERO_RS02115: HSERO_RS02115 - valyl-tRNA synthetase, at 459,335 to 462,145
_RS02115
HSERO_RS02120: HSERO_RS02120 - phosphomethylpyrimidine synthase ThiC, at 462,763 to 464,676
_RS02120
HSERO_RS02125: HSERO_RS02125 - thiamine biosynthesis protein ThiS, at 464,682 to 464,882
_RS02125
HSERO_RS02130: HSERO_RS02130 - thiazole synthase, at 464,921 to 465,730
_RS02130
Position (kb) 462
463
464
465 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 462.209 kb on + strand at 462.210 kb on - strand at 462.347 kb on - strand at 462.366 kb on + strand at 462.454 kb on + strand at 462.462 kb on - strand at 462.467 kb on + strand at 462.687 kb on + strand at 462.862 kb on + strand at 462.898 kb on - strand at 462.937 kb on - strand at 462.959 kb on + strand, within HSERO_RS02120 at 463.027 kb on + strand, within HSERO_RS02120 at 463.124 kb on + strand, within HSERO_RS02120 at 463.221 kb on + strand, within HSERO_RS02120 at 463.229 kb on - strand, within HSERO_RS02120 at 463.259 kb on + strand, within HSERO_RS02120 at 463.325 kb on + strand, within HSERO_RS02120 at 463.501 kb on + strand, within HSERO_RS02120 at 463.945 kb on - strand, within HSERO_RS02120 at 464.053 kb on + strand, within HSERO_RS02120 at 464.114 kb on - strand, within HSERO_RS02120 at 464.116 kb on - strand, within HSERO_RS02120 at 464.161 kb on - strand, within HSERO_RS02120 at 464.250 kb on + strand, within HSERO_RS02120 at 464.280 kb on - strand, within HSERO_RS02120 at 464.281 kb on - strand, within HSERO_RS02120 at 464.291 kb on - strand, within HSERO_RS02120 at 464.389 kb on - strand, within HSERO_RS02120 at 464.399 kb on - strand, within HSERO_RS02120 at 464.399 kb on - strand, within HSERO_RS02120 at 464.399 kb on - strand, within HSERO_RS02120 at 464.417 kb on + strand, within HSERO_RS02120 at 464.503 kb on + strand at 464.508 kb on + strand at 464.532 kb on + strand at 464.552 kb on + strand at 464.560 kb on - strand at 464.622 kb on + strand at 464.627 kb on + strand at 464.739 kb on - strand, within HSERO_RS02125 at 464.741 kb on + strand, within HSERO_RS02125 at 464.749 kb on - strand, within HSERO_RS02125 at 464.752 kb on + strand, within HSERO_RS02125 at 464.834 kb on + strand, within HSERO_RS02125 at 464.834 kb on + strand, within HSERO_RS02125 at 464.849 kb on + strand, within HSERO_RS02125 at 464.959 kb on + strand at 464.960 kb on + strand at 464.968 kb on - strand at 465.016 kb on + strand, within HSERO_RS02130 at 465.163 kb on + strand, within HSERO_RS02130 at 465.255 kb on - strand, within HSERO_RS02130 at 465.286 kb on + strand, within HSERO_RS02130 at 465.311 kb on - strand, within HSERO_RS02130 at 465.393 kb on + strand, within HSERO_RS02130 at 465.448 kb on - strand, within HSERO_RS02130 at 465.584 kb on + strand, within HSERO_RS02130
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Malic Acid (C) remove 462,209 + +1.4 462,210 - +0.5 462,347 - -0.2 462,366 + +0.2 462,454 + +0.5 462,462 - -0.4 462,467 + +0.9 462,687 + -2.3 462,862 + -3.3 462,898 - -3.8 462,937 - -2.4 462,959 + HSERO_RS02120 0.10 -2.8 463,027 + HSERO_RS02120 0.14 -2.8 463,124 + HSERO_RS02120 0.19 -4.1 463,221 + HSERO_RS02120 0.24 -0.4 463,229 - HSERO_RS02120 0.24 -2.2 463,259 + HSERO_RS02120 0.26 -2.5 463,325 + HSERO_RS02120 0.29 -2.6 463,501 + HSERO_RS02120 0.39 -1.4 463,945 - HSERO_RS02120 0.62 -2.8 464,053 + HSERO_RS02120 0.67 -3.1 464,114 - HSERO_RS02120 0.71 -1.6 464,116 - HSERO_RS02120 0.71 -5.8 464,161 - HSERO_RS02120 0.73 -2.7 464,250 + HSERO_RS02120 0.78 -4.0 464,280 - HSERO_RS02120 0.79 -2.8 464,281 - HSERO_RS02120 0.79 -2.7 464,291 - HSERO_RS02120 0.80 -2.6 464,389 - HSERO_RS02120 0.85 -1.2 464,399 - HSERO_RS02120 0.85 -2.6 464,399 - HSERO_RS02120 0.85 -2.6 464,399 - HSERO_RS02120 0.85 -2.3 464,417 + HSERO_RS02120 0.86 -2.5 464,503 + +0.1 464,508 + -1.1 464,532 + -2.1 464,552 + +0.6 464,560 - -2.9 464,622 + +0.2 464,627 + +0.7 464,739 - HSERO_RS02125 0.28 -2.1 464,741 + HSERO_RS02125 0.29 -3.4 464,749 - HSERO_RS02125 0.33 -3.7 464,752 + HSERO_RS02125 0.35 -2.9 464,834 + HSERO_RS02125 0.76 -2.7 464,834 + HSERO_RS02125 0.76 -2.8 464,849 + HSERO_RS02125 0.83 -1.4 464,959 + -2.6 464,960 + -2.0 464,968 - -1.8 465,016 + HSERO_RS02130 0.12 -2.1 465,163 + HSERO_RS02130 0.30 -4.4 465,255 - HSERO_RS02130 0.41 -3.0 465,286 + HSERO_RS02130 0.45 -2.2 465,311 - HSERO_RS02130 0.48 -0.8 465,393 + HSERO_RS02130 0.58 -0.9 465,448 - HSERO_RS02130 0.65 -2.0 465,584 + HSERO_RS02130 0.82 -1.4
Or see this region's nucleotide sequence