Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS01415

Experiment: soil sample 3; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS01410 and ABZR87_RS01415 are separated by 175 nucleotidesABZR87_RS01415 and ABZR87_RS01420 are separated by 122 nucleotidesABZR87_RS01420 and ABZR87_RS01425 are separated by 152 nucleotides ABZR87_RS01410: ABZR87_RS01410 - leucine--tRNA ligase, at 294,796 to 297,429 _RS01410 ABZR87_RS01415: ABZR87_RS01415 - 4-hydroxy-tetrahydrodipicolinate reductase, at 297,605 to 298,396 _RS01415 ABZR87_RS01420: ABZR87_RS01420 - outer membrane protein assembly factor BamE, at 298,519 to 299,175 _RS01420 ABZR87_RS01425: ABZR87_RS01425 - ferric iron uptake transcriptional regulator, at 299,328 to 299,759 _RS01425 Position (kb) 297 298 299Strain fitness (log2 ratio) -2 -1 0 1 2at 297.515 kb on - strandat 297.515 kb on - strandat 297.515 kb on - strandat 297.516 kb on + strandat 297.516 kb on + strandat 297.516 kb on + strandat 298.436 kb on + strandat 298.436 kb on + strandat 298.560 kb on + strandat 298.560 kb on + strandat 298.561 kb on - strandat 298.561 kb on - strandat 298.561 kb on - strandat 298.561 kb on - strandat 298.878 kb on - strand, within ABZR87_RS01420at 298.878 kb on - strand, within ABZR87_RS01420at 299.060 kb on + strand, within ABZR87_RS01420at 299.061 kb on - strand, within ABZR87_RS01420at 299.061 kb on - strand, within ABZR87_RS01420at 299.207 kb on - strandat 299.212 kb on + strandat 299.261 kb on - strandat 299.308 kb on + strandat 299.308 kb on + strandat 299.308 kb on + strandat 299.309 kb on - strandat 299.309 kb on - strandat 299.313 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction soil sample 3; outgrowth in LB
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297,515 - +0.8
297,515 - +0.6
297,515 - -0.7
297,516 + +0.5
297,516 + -0.8
297,516 + +0.8
298,436 + -0.7
298,436 + -0.2
298,560 + +0.8
298,560 + -1.9
298,561 - +0.3
298,561 - -0.2
298,561 - +0.3
298,561 - -1.2
298,878 - ABZR87_RS01420 0.55 +1.8
298,878 - ABZR87_RS01420 0.55 +0.3
299,060 + ABZR87_RS01420 0.82 +0.3
299,061 - ABZR87_RS01420 0.82 +1.0
299,061 - ABZR87_RS01420 0.82 -1.1
299,207 - -0.2
299,212 + +2.5
299,261 - +0.3
299,308 + -1.0
299,308 + -2.2
299,308 + -2.3
299,309 - -0.4
299,309 - -1.2
299,313 + -0.5

Or see this region's nucleotide sequence