Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33235 and RR42_RS33240 are separated by 29 nucleotides RR42_RS33240 and RR42_RS33245 are separated by 8 nucleotides RR42_RS33245 and RR42_RS33250 are separated by 305 nucleotides
RR42_RS33235: RR42_RS33235 - GDP-mannose 4,6-dehydratase, at 2,870,466 to 2,871,554
_RS33235
RR42_RS33240: RR42_RS33240 - GDP-L-fucose synthase, at 2,871,584 to 2,872,516
_RS33240
RR42_RS33245: RR42_RS33245 - mannose-1-phosphate guanyltransferase, at 2,872,525 to 2,873,949
_RS33245
RR42_RS33250: RR42_RS33250 - tyrosine protein kinase, at 2,874,255 to 2,876,510
_RS33250
Position (kb)
2872
2873
2874 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2871.563 kb on - strand at 2871.582 kb on - strand at 2871.585 kb on + strand at 2871.586 kb on - strand at 2871.681 kb on - strand, within RR42_RS33240 at 2871.726 kb on + strand, within RR42_RS33240 at 2871.726 kb on + strand, within RR42_RS33240 at 2871.727 kb on - strand, within RR42_RS33240 at 2871.728 kb on + strand, within RR42_RS33240 at 2871.788 kb on + strand, within RR42_RS33240 at 2871.789 kb on - strand, within RR42_RS33240 at 2871.803 kb on - strand, within RR42_RS33240 at 2871.807 kb on - strand, within RR42_RS33240 at 2871.808 kb on + strand, within RR42_RS33240 at 2871.809 kb on - strand, within RR42_RS33240 at 2871.812 kb on + strand, within RR42_RS33240 at 2871.813 kb on - strand, within RR42_RS33240 at 2871.828 kb on + strand, within RR42_RS33240 at 2871.829 kb on - strand, within RR42_RS33240 at 2871.848 kb on + strand, within RR42_RS33240 at 2871.848 kb on + strand, within RR42_RS33240 at 2871.849 kb on - strand, within RR42_RS33240 at 2871.849 kb on - strand, within RR42_RS33240 at 2871.872 kb on + strand, within RR42_RS33240 at 2871.872 kb on + strand, within RR42_RS33240 at 2871.873 kb on - strand, within RR42_RS33240 at 2871.912 kb on - strand, within RR42_RS33240 at 2871.918 kb on + strand, within RR42_RS33240 at 2871.936 kb on - strand, within RR42_RS33240 at 2871.936 kb on - strand, within RR42_RS33240 at 2871.972 kb on - strand, within RR42_RS33240 at 2871.982 kb on + strand, within RR42_RS33240 at 2871.982 kb on + strand, within RR42_RS33240 at 2871.983 kb on - strand, within RR42_RS33240 at 2871.983 kb on - strand, within RR42_RS33240 at 2871.990 kb on - strand, within RR42_RS33240 at 2871.990 kb on - strand, within RR42_RS33240 at 2871.997 kb on - strand, within RR42_RS33240 at 2871.997 kb on - strand, within RR42_RS33240 at 2871.997 kb on - strand, within RR42_RS33240 at 2871.997 kb on - strand, within RR42_RS33240 at 2872.037 kb on + strand, within RR42_RS33240 at 2872.038 kb on - strand, within RR42_RS33240 at 2872.038 kb on - strand, within RR42_RS33240 at 2872.041 kb on + strand, within RR42_RS33240 at 2872.041 kb on + strand, within RR42_RS33240 at 2872.042 kb on - strand, within RR42_RS33240 at 2872.042 kb on - strand, within RR42_RS33240 at 2872.065 kb on + strand, within RR42_RS33240 at 2872.065 kb on + strand, within RR42_RS33240 at 2872.066 kb on - strand, within RR42_RS33240 at 2872.108 kb on - strand, within RR42_RS33240 at 2872.146 kb on + strand, within RR42_RS33240 at 2872.146 kb on + strand, within RR42_RS33240 at 2872.147 kb on - strand, within RR42_RS33240 at 2872.147 kb on - strand, within RR42_RS33240 at 2872.182 kb on + strand, within RR42_RS33240 at 2872.182 kb on + strand, within RR42_RS33240 at 2872.186 kb on + strand, within RR42_RS33240 at 2872.186 kb on + strand, within RR42_RS33240 at 2872.186 kb on + strand, within RR42_RS33240 at 2872.186 kb on + strand, within RR42_RS33240 at 2872.187 kb on - strand, within RR42_RS33240 at 2872.187 kb on - strand, within RR42_RS33240 at 2872.187 kb on - strand, within RR42_RS33240 at 2872.290 kb on + strand, within RR42_RS33240 at 2872.291 kb on - strand, within RR42_RS33240 at 2872.292 kb on + strand, within RR42_RS33240 at 2872.292 kb on + strand, within RR42_RS33240 at 2872.293 kb on - strand, within RR42_RS33240 at 2872.293 kb on - strand, within RR42_RS33240 at 2872.342 kb on - strand, within RR42_RS33240 at 2872.397 kb on + strand, within RR42_RS33240 at 2872.397 kb on + strand, within RR42_RS33240 at 2872.398 kb on - strand, within RR42_RS33240 at 2872.398 kb on - strand, within RR42_RS33240 at 2872.431 kb on + strand at 2872.459 kb on + strand at 2872.459 kb on + strand at 2872.459 kb on + strand at 2872.460 kb on - strand at 2872.510 kb on + strand at 2872.510 kb on + strand at 2872.511 kb on - strand at 2872.538 kb on + strand at 2872.539 kb on - strand at 2872.577 kb on + strand at 2872.578 kb on - strand at 2872.656 kb on + strand at 2872.659 kb on - strand at 2872.682 kb on - strand, within RR42_RS33245 at 2872.809 kb on - strand, within RR42_RS33245 at 2872.836 kb on - strand, within RR42_RS33245 at 2872.854 kb on - strand, within RR42_RS33245 at 2872.942 kb on + strand, within RR42_RS33245 at 2872.943 kb on - strand, within RR42_RS33245 at 2872.978 kb on + strand, within RR42_RS33245 at 2872.978 kb on + strand, within RR42_RS33245 at 2872.978 kb on + strand, within RR42_RS33245 at 2872.978 kb on + strand, within RR42_RS33245 at 2872.979 kb on - strand, within RR42_RS33245 at 2873.169 kb on + strand, within RR42_RS33245 at 2873.169 kb on + strand, within RR42_RS33245 at 2873.186 kb on + strand, within RR42_RS33245 at 2873.291 kb on - strand, within RR42_RS33245 at 2873.320 kb on + strand, within RR42_RS33245 at 2873.324 kb on + strand, within RR42_RS33245 at 2873.325 kb on - strand, within RR42_RS33245 at 2873.325 kb on - strand, within RR42_RS33245 at 2873.325 kb on - strand, within RR42_RS33245 at 2873.366 kb on + strand, within RR42_RS33245 at 2873.367 kb on - strand, within RR42_RS33245 at 2873.367 kb on - strand, within RR42_RS33245 at 2873.385 kb on - strand, within RR42_RS33245 at 2873.471 kb on + strand, within RR42_RS33245 at 2873.587 kb on + strand, within RR42_RS33245 at 2873.588 kb on - strand, within RR42_RS33245 at 2873.673 kb on + strand, within RR42_RS33245 at 2873.918 kb on - strand at 2874.059 kb on - strand at 2874.223 kb on - strand at 2874.299 kb on - strand at 2874.304 kb on + strand at 2874.308 kb on - strand at 2874.308 kb on - strand at 2874.340 kb on + strand at 2874.465 kb on - strand at 2874.522 kb on + strand, within RR42_RS33250 at 2874.522 kb on + strand, within RR42_RS33250 at 2874.546 kb on + strand, within RR42_RS33250 at 2874.582 kb on + strand, within RR42_RS33250 at 2874.660 kb on + strand, within RR42_RS33250 at 2874.668 kb on + strand, within RR42_RS33250 at 2874.705 kb on + strand, within RR42_RS33250 at 2874.879 kb on - strand, within RR42_RS33250 at 2874.881 kb on + strand, within RR42_RS33250 at 2874.881 kb on + strand, within RR42_RS33250 at 2874.926 kb on + strand, within RR42_RS33250
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 2,871,563 - -0.6 2,871,582 - -0.1 2,871,585 + -2.6 2,871,586 - +0.8 2,871,681 - RR42_RS33240 0.10 -2.8 2,871,726 + RR42_RS33240 0.15 -3.6 2,871,726 + RR42_RS33240 0.15 -2.4 2,871,727 - RR42_RS33240 0.15 -2.3 2,871,728 + RR42_RS33240 0.15 -2.3 2,871,788 + RR42_RS33240 0.22 -3.1 2,871,789 - RR42_RS33240 0.22 -3.8 2,871,803 - RR42_RS33240 0.23 -3.2 2,871,807 - RR42_RS33240 0.24 -1.3 2,871,808 + RR42_RS33240 0.24 -1.3 2,871,809 - RR42_RS33240 0.24 -1.8 2,871,812 + RR42_RS33240 0.24 -5.1 2,871,813 - RR42_RS33240 0.25 -2.6 2,871,828 + RR42_RS33240 0.26 +0.7 2,871,829 - RR42_RS33240 0.26 -2.4 2,871,848 + RR42_RS33240 0.28 -4.8 2,871,848 + RR42_RS33240 0.28 -1.3 2,871,849 - RR42_RS33240 0.28 -3.2 2,871,849 - RR42_RS33240 0.28 -3.0 2,871,872 + RR42_RS33240 0.31 -1.3 2,871,872 + RR42_RS33240 0.31 -3.0 2,871,873 - RR42_RS33240 0.31 -4.0 2,871,912 - RR42_RS33240 0.35 -6.6 2,871,918 + RR42_RS33240 0.36 -0.8 2,871,936 - RR42_RS33240 0.38 -4.1 2,871,936 - RR42_RS33240 0.38 -4.4 2,871,972 - RR42_RS33240 0.42 -5.2 2,871,982 + RR42_RS33240 0.43 -3.8 2,871,982 + RR42_RS33240 0.43 -1.3 2,871,983 - RR42_RS33240 0.43 -2.3 2,871,983 - RR42_RS33240 0.43 -3.6 2,871,990 - RR42_RS33240 0.44 -3.3 2,871,990 - RR42_RS33240 0.44 -2.3 2,871,997 - RR42_RS33240 0.44 -3.0 2,871,997 - RR42_RS33240 0.44 -4.6 2,871,997 - RR42_RS33240 0.44 -5.5 2,871,997 - RR42_RS33240 0.44 -3.2 2,872,037 + RR42_RS33240 0.49 -5.2 2,872,038 - RR42_RS33240 0.49 -1.0 2,872,038 - RR42_RS33240 0.49 -0.8 2,872,041 + RR42_RS33240 0.49 -2.4 2,872,041 + RR42_RS33240 0.49 -2.1 2,872,042 - RR42_RS33240 0.49 -1.3 2,872,042 - RR42_RS33240 0.49 -3.2 2,872,065 + RR42_RS33240 0.52 -5.5 2,872,065 + RR42_RS33240 0.52 -3.3 2,872,066 - RR42_RS33240 0.52 -2.8 2,872,108 - RR42_RS33240 0.56 -2.4 2,872,146 + RR42_RS33240 0.60 -3.3 2,872,146 + RR42_RS33240 0.60 -2.7 2,872,147 - RR42_RS33240 0.60 -2.6 2,872,147 - RR42_RS33240 0.60 -5.8 2,872,182 + RR42_RS33240 0.64 -4.3 2,872,182 + RR42_RS33240 0.64 -4.1 2,872,186 + RR42_RS33240 0.65 -2.8 2,872,186 + RR42_RS33240 0.65 -1.3 2,872,186 + RR42_RS33240 0.65 -4.7 2,872,186 + RR42_RS33240 0.65 -1.3 2,872,187 - RR42_RS33240 0.65 -4.1 2,872,187 - RR42_RS33240 0.65 -4.3 2,872,187 - RR42_RS33240 0.65 -4.0 2,872,290 + RR42_RS33240 0.76 -1.8 2,872,291 - RR42_RS33240 0.76 -1.3 2,872,292 + RR42_RS33240 0.76 -5.7 2,872,292 + RR42_RS33240 0.76 -2.3 2,872,293 - RR42_RS33240 0.76 -3.1 2,872,293 - RR42_RS33240 0.76 -3.3 2,872,342 - RR42_RS33240 0.81 -6.4 2,872,397 + RR42_RS33240 0.87 -1.3 2,872,397 + RR42_RS33240 0.87 -4.4 2,872,398 - RR42_RS33240 0.87 -0.8 2,872,398 - RR42_RS33240 0.87 -5.6 2,872,431 + -2.3 2,872,459 + -2.3 2,872,459 + -2.6 2,872,459 + -0.3 2,872,460 - -4.5 2,872,510 + -1.3 2,872,510 + -2.4 2,872,511 - -4.1 2,872,538 + -3.5 2,872,539 - -1.6 2,872,577 + -1.8 2,872,578 - -3.8 2,872,656 + -2.7 2,872,659 - -4.3 2,872,682 - RR42_RS33245 0.11 +0.7 2,872,809 - RR42_RS33245 0.20 -3.2 2,872,836 - RR42_RS33245 0.22 -4.2 2,872,854 - RR42_RS33245 0.23 -4.0 2,872,942 + RR42_RS33245 0.29 -3.2 2,872,943 - RR42_RS33245 0.29 -0.8 2,872,978 + RR42_RS33245 0.32 -3.8 2,872,978 + RR42_RS33245 0.32 -0.8 2,872,978 + RR42_RS33245 0.32 -3.0 2,872,978 + RR42_RS33245 0.32 -2.4 2,872,979 - RR42_RS33245 0.32 +0.7 2,873,169 + RR42_RS33245 0.45 -3.0 2,873,169 + RR42_RS33245 0.45 -2.1 2,873,186 + RR42_RS33245 0.46 -2.3 2,873,291 - RR42_RS33245 0.54 -1.3 2,873,320 + RR42_RS33245 0.56 -4.6 2,873,324 + RR42_RS33245 0.56 -3.5 2,873,325 - RR42_RS33245 0.56 -4.3 2,873,325 - RR42_RS33245 0.56 -6.1 2,873,325 - RR42_RS33245 0.56 -1.3 2,873,366 + RR42_RS33245 0.59 -2.3 2,873,367 - RR42_RS33245 0.59 -3.5 2,873,367 - RR42_RS33245 0.59 -4.6 2,873,385 - RR42_RS33245 0.60 -3.7 2,873,471 + RR42_RS33245 0.66 -1.2 2,873,587 + RR42_RS33245 0.75 -4.3 2,873,588 - RR42_RS33245 0.75 -2.6 2,873,673 + RR42_RS33245 0.81 -1.1 2,873,918 - -5.1 2,874,059 - -1.0 2,874,223 - +0.1 2,874,299 - -1.0 2,874,304 + -5.0 2,874,308 - -1.8 2,874,308 - -4.3 2,874,340 + -3.1 2,874,465 - -4.8 2,874,522 + RR42_RS33250 0.12 -1.3 2,874,522 + RR42_RS33250 0.12 -3.1 2,874,546 + RR42_RS33250 0.13 -0.3 2,874,582 + RR42_RS33250 0.14 -4.5 2,874,660 + RR42_RS33250 0.18 -3.2 2,874,668 + RR42_RS33250 0.18 -3.1 2,874,705 + RR42_RS33250 0.20 -2.3 2,874,879 - RR42_RS33250 0.28 -4.8 2,874,881 + RR42_RS33250 0.28 -2.3 2,874,881 + RR42_RS33250 0.28 -3.3 2,874,926 + RR42_RS33250 0.30 -3.0
Or see this region's nucleotide sequence