Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27035 and RR42_RS27040 are separated by 3 nucleotides RR42_RS27040 and RR42_RS27045 are separated by 87 nucleotides RR42_RS27045 and RR42_RS27050 are separated by 80 nucleotides RR42_RS27050 and RR42_RS27055 are separated by 10 nucleotides
RR42_RS27035: RR42_RS27035 - hypothetical protein, at 1,418,652 to 1,418,831
_RS27035
RR42_RS27040: RR42_RS27040 - hypothetical protein, at 1,418,835 to 1,419,020
_RS27040
RR42_RS27045: RR42_RS27045 - hypothetical protein, at 1,419,108 to 1,419,506
_RS27045
RR42_RS27050: RR42_RS27050 - transporter, at 1,419,587 to 1,420,960
_RS27050
RR42_RS27055: RR42_RS27055 - membrane protein, at 1,420,971 to 1,424,111
_RS27055
Position (kb)
1419
1420
1421 Strain fitness (log2 ratio)
-2
-1
0
1 at 1418.616 kb on + strand at 1418.616 kb on + strand at 1418.617 kb on - strand at 1418.617 kb on - strand at 1418.621 kb on + strand at 1418.621 kb on + strand at 1418.744 kb on + strand, within RR42_RS27035 at 1418.745 kb on - strand, within RR42_RS27035 at 1418.751 kb on - strand, within RR42_RS27035 at 1418.751 kb on - strand, within RR42_RS27035 at 1419.317 kb on - strand, within RR42_RS27045 at 1419.376 kb on + strand, within RR42_RS27045 at 1419.417 kb on + strand, within RR42_RS27045 at 1419.537 kb on + strand at 1419.663 kb on + strand at 1419.663 kb on + strand at 1419.664 kb on - strand at 1419.664 kb on - strand at 1419.672 kb on + strand at 1419.673 kb on - strand at 1420.206 kb on + strand, within RR42_RS27050 at 1420.507 kb on - strand, within RR42_RS27050 at 1420.683 kb on + strand, within RR42_RS27050 at 1420.683 kb on + strand, within RR42_RS27050 at 1420.684 kb on - strand, within RR42_RS27050 at 1421.077 kb on + strand at 1421.078 kb on - strand at 1421.876 kb on - strand, within RR42_RS27055
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 1,418,616 + -0.3 1,418,616 + +0.0 1,418,617 - -0.3 1,418,617 - +0.1 1,418,621 + +0.5 1,418,621 + +1.0 1,418,744 + RR42_RS27035 0.51 -1.7 1,418,745 - RR42_RS27035 0.52 -1.4 1,418,751 - RR42_RS27035 0.55 -0.6 1,418,751 - RR42_RS27035 0.55 -0.1 1,419,317 - RR42_RS27045 0.52 -0.7 1,419,376 + RR42_RS27045 0.67 -1.0 1,419,417 + RR42_RS27045 0.77 +0.0 1,419,537 + +0.0 1,419,663 + +0.5 1,419,663 + -1.5 1,419,664 - -2.5 1,419,664 - -2.1 1,419,672 + -0.1 1,419,673 - +0.0 1,420,206 + RR42_RS27050 0.45 -1.0 1,420,507 - RR42_RS27050 0.67 -0.2 1,420,683 + RR42_RS27050 0.80 -1.4 1,420,683 + RR42_RS27050 0.80 -0.7 1,420,684 - RR42_RS27050 0.80 -2.4 1,421,077 + -0.8 1,421,078 - -0.1 1,421,876 - RR42_RS27055 0.29 -1.0
Or see this region's nucleotide sequence