Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25535

Experiment: D-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25525 and RR42_RS25530 are separated by 158 nucleotidesRR42_RS25530 and RR42_RS25535 are separated by 132 nucleotidesRR42_RS25535 and RR42_RS25540 are separated by 419 nucleotidesRR42_RS25540 and RR42_RS25545 are separated by 8 nucleotides RR42_RS25525: RR42_RS25525 - enoyl-CoA hydratase, at 1,072,307 to 1,073,281 _RS25525 RR42_RS25530: RR42_RS25530 - TetR family transcriptional regulator, at 1,073,440 to 1,074,090 _RS25530 RR42_RS25535: RR42_RS25535 - TetR family transcriptional regulator, at 1,074,223 to 1,074,834 _RS25535 RR42_RS25540: RR42_RS25540 - hypothetical protein, at 1,075,254 to 1,075,718 _RS25540 RR42_RS25545: RR42_RS25545 - NADPH:quinone oxidoreductase, at 1,075,727 to 1,076,728 _RS25545 Position (kb) 1074 1075Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1073.243 kb on + strandat 1073.243 kb on + strandat 1073.243 kb on + strandat 1073.244 kb on - strandat 1073.409 kb on + strandat 1073.439 kb on + strandat 1073.440 kb on - strandat 1073.440 kb on - strandat 1073.451 kb on + strandat 1073.578 kb on + strand, within RR42_RS25530at 1073.578 kb on + strand, within RR42_RS25530at 1073.578 kb on + strand, within RR42_RS25530at 1073.579 kb on - strand, within RR42_RS25530at 1073.693 kb on + strand, within RR42_RS25530at 1073.693 kb on + strand, within RR42_RS25530at 1073.746 kb on + strand, within RR42_RS25530at 1073.782 kb on + strand, within RR42_RS25530at 1073.782 kb on + strand, within RR42_RS25530at 1073.783 kb on - strand, within RR42_RS25530at 1073.783 kb on - strand, within RR42_RS25530at 1073.783 kb on - strand, within RR42_RS25530at 1073.783 kb on - strand, within RR42_RS25530at 1073.784 kb on + strand, within RR42_RS25530at 1073.854 kb on + strand, within RR42_RS25530at 1073.855 kb on - strand, within RR42_RS25530at 1073.933 kb on + strand, within RR42_RS25530at 1073.933 kb on + strand, within RR42_RS25530at 1074.332 kb on - strand, within RR42_RS25535at 1074.392 kb on - strand, within RR42_RS25535at 1074.526 kb on + strand, within RR42_RS25535at 1074.526 kb on + strand, within RR42_RS25535at 1074.527 kb on - strand, within RR42_RS25535at 1074.527 kb on - strand, within RR42_RS25535at 1074.581 kb on + strand, within RR42_RS25535at 1074.582 kb on - strand, within RR42_RS25535at 1074.656 kb on + strand, within RR42_RS25535at 1074.656 kb on + strand, within RR42_RS25535at 1074.657 kb on - strand, within RR42_RS25535at 1074.657 kb on - strand, within RR42_RS25535at 1074.724 kb on - strand, within RR42_RS25535at 1074.724 kb on - strand, within RR42_RS25535at 1075.015 kb on - strandat 1075.081 kb on + strandat 1075.081 kb on + strandat 1075.081 kb on + strandat 1075.081 kb on + strandat 1075.081 kb on + strandat 1075.082 kb on - strandat 1075.092 kb on + strandat 1075.093 kb on - strandat 1075.093 kb on - strandat 1075.145 kb on + strandat 1075.146 kb on - strandat 1075.146 kb on - strandat 1075.146 kb on - strandat 1075.146 kb on - strandat 1075.150 kb on - strandat 1075.150 kb on - strandat 1075.150 kb on - strandat 1075.441 kb on + strand, within RR42_RS25540at 1075.442 kb on - strand, within RR42_RS25540at 1075.569 kb on + strand, within RR42_RS25540at 1075.570 kb on - strand, within RR42_RS25540at 1075.570 kb on - strand, within RR42_RS25540at 1075.570 kb on - strand, within RR42_RS25540at 1075.570 kb on - strand, within RR42_RS25540at 1075.585 kb on + strand, within RR42_RS25540at 1075.585 kb on + strand, within RR42_RS25540at 1075.666 kb on - strand, within RR42_RS25540

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (N)
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1,073,243 + +0.2
1,073,243 + -1.6
1,073,243 + +0.7
1,073,244 - -0.2
1,073,409 + -0.1
1,073,439 + +0.2
1,073,440 - -1.2
1,073,440 - -0.3
1,073,451 + +1.3
1,073,578 + RR42_RS25530 0.21 -0.4
1,073,578 + RR42_RS25530 0.21 -1.0
1,073,578 + RR42_RS25530 0.21 -0.7
1,073,579 - RR42_RS25530 0.21 -0.9
1,073,693 + RR42_RS25530 0.39 -0.3
1,073,693 + RR42_RS25530 0.39 -0.7
1,073,746 + RR42_RS25530 0.47 -4.0
1,073,782 + RR42_RS25530 0.53 +0.7
1,073,782 + RR42_RS25530 0.53 +0.7
1,073,783 - RR42_RS25530 0.53 -1.9
1,073,783 - RR42_RS25530 0.53 +0.2
1,073,783 - RR42_RS25530 0.53 -0.7
1,073,783 - RR42_RS25530 0.53 -1.0
1,073,784 + RR42_RS25530 0.53 -0.3
1,073,854 + RR42_RS25530 0.64 +0.1
1,073,855 - RR42_RS25530 0.64 -1.9
1,073,933 + RR42_RS25530 0.76 -1.5
1,073,933 + RR42_RS25530 0.76 -0.7
1,074,332 - RR42_RS25535 0.18 -0.2
1,074,392 - RR42_RS25535 0.28 -1.3
1,074,526 + RR42_RS25535 0.50 +0.5
1,074,526 + RR42_RS25535 0.50 -0.0
1,074,527 - RR42_RS25535 0.50 -0.2
1,074,527 - RR42_RS25535 0.50 -0.4
1,074,581 + RR42_RS25535 0.58 +0.4
1,074,582 - RR42_RS25535 0.59 -1.2
1,074,656 + RR42_RS25535 0.71 -0.6
1,074,656 + RR42_RS25535 0.71 -0.1
1,074,657 - RR42_RS25535 0.71 +0.8
1,074,657 - RR42_RS25535 0.71 +0.9
1,074,724 - RR42_RS25535 0.82 -1.8
1,074,724 - RR42_RS25535 0.82 -0.0
1,075,015 - -0.2
1,075,081 + -0.6
1,075,081 + +1.0
1,075,081 + -0.6
1,075,081 + -0.9
1,075,081 + +0.2
1,075,082 - +0.8
1,075,092 + +0.5
1,075,093 - +1.0
1,075,093 - -0.3
1,075,145 + -0.3
1,075,146 - +0.6
1,075,146 - +0.3
1,075,146 - +0.3
1,075,146 - -0.9
1,075,150 - -0.1
1,075,150 - -0.3
1,075,150 - -0.9
1,075,441 + RR42_RS25540 0.40 +1.5
1,075,442 - RR42_RS25540 0.40 +0.3
1,075,569 + RR42_RS25540 0.68 -0.0
1,075,570 - RR42_RS25540 0.68 -0.2
1,075,570 - RR42_RS25540 0.68 +0.1
1,075,570 - RR42_RS25540 0.68 +0.3
1,075,570 - RR42_RS25540 0.68 -0.7
1,075,585 + RR42_RS25540 0.71 +0.9
1,075,585 + RR42_RS25540 0.71 +0.1
1,075,666 - RR42_RS25540 0.89 +1.6

Or see this region's nucleotide sequence