Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25520

Experiment: D-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25515 and RR42_RS25520 are separated by 50 nucleotidesRR42_RS25520 and RR42_RS25525 overlap by 4 nucleotides RR42_RS25515: RR42_RS25515 - AMP-dependent synthetase, at 1,069,867 to 1,071,327 _RS25515 RR42_RS25520: RR42_RS25520 - alpha/beta hydrolase, at 1,071,378 to 1,072,310 _RS25520 RR42_RS25525: RR42_RS25525 - enoyl-CoA hydratase, at 1,072,307 to 1,073,281 _RS25525 Position (kb) 1071 1072 1073Strain fitness (log2 ratio) -1 0 1at 1070.501 kb on + strand, within RR42_RS25515at 1070.501 kb on + strand, within RR42_RS25515at 1070.501 kb on + strand, within RR42_RS25515at 1070.502 kb on - strand, within RR42_RS25515at 1070.618 kb on + strand, within RR42_RS25515at 1070.619 kb on - strand, within RR42_RS25515at 1070.642 kb on + strand, within RR42_RS25515at 1070.643 kb on - strand, within RR42_RS25515at 1070.643 kb on - strand, within RR42_RS25515at 1070.897 kb on + strand, within RR42_RS25515at 1070.898 kb on - strand, within RR42_RS25515at 1071.018 kb on + strand, within RR42_RS25515at 1071.340 kb on + strandat 1071.340 kb on + strandat 1071.341 kb on - strandat 1071.344 kb on + strandat 1071.454 kb on + strandat 1071.455 kb on - strandat 1071.685 kb on + strand, within RR42_RS25520at 1071.807 kb on - strand, within RR42_RS25520at 1071.840 kb on - strand, within RR42_RS25520at 1071.942 kb on - strand, within RR42_RS25520at 1072.017 kb on + strand, within RR42_RS25520at 1072.018 kb on - strand, within RR42_RS25520at 1072.079 kb on - strand, within RR42_RS25520at 1072.079 kb on - strand, within RR42_RS25520at 1072.229 kb on - strandat 1072.365 kb on + strandat 1072.522 kb on - strand, within RR42_RS25525at 1072.573 kb on - strand, within RR42_RS25525at 1072.760 kb on + strand, within RR42_RS25525at 1072.760 kb on + strand, within RR42_RS25525at 1072.761 kb on - strand, within RR42_RS25525at 1072.807 kb on + strand, within RR42_RS25525at 1073.028 kb on - strand, within RR42_RS25525at 1073.111 kb on + strand, within RR42_RS25525at 1073.112 kb on - strand, within RR42_RS25525at 1073.243 kb on + strandat 1073.243 kb on + strandat 1073.243 kb on + strandat 1073.244 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (N)
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1,070,501 + RR42_RS25515 0.43 +0.4
1,070,501 + RR42_RS25515 0.43 +1.3
1,070,501 + RR42_RS25515 0.43 +0.5
1,070,502 - RR42_RS25515 0.43 -1.3
1,070,618 + RR42_RS25515 0.51 +0.8
1,070,619 - RR42_RS25515 0.51 -0.2
1,070,642 + RR42_RS25515 0.53 -0.3
1,070,643 - RR42_RS25515 0.53 -0.3
1,070,643 - RR42_RS25515 0.53 -0.5
1,070,897 + RR42_RS25515 0.70 -0.1
1,070,898 - RR42_RS25515 0.71 +1.2
1,071,018 + RR42_RS25515 0.79 -0.6
1,071,340 + +0.7
1,071,340 + -0.7
1,071,341 - -0.0
1,071,344 + +0.1
1,071,454 + -1.2
1,071,455 - +0.0
1,071,685 + RR42_RS25520 0.33 -0.6
1,071,807 - RR42_RS25520 0.46 +0.2
1,071,840 - RR42_RS25520 0.50 -0.7
1,071,942 - RR42_RS25520 0.60 +0.7
1,072,017 + RR42_RS25520 0.68 -0.3
1,072,018 - RR42_RS25520 0.69 -0.3
1,072,079 - RR42_RS25520 0.75 -0.1
1,072,079 - RR42_RS25520 0.75 +0.6
1,072,229 - -0.5
1,072,365 + +0.1
1,072,522 - RR42_RS25525 0.22 +0.3
1,072,573 - RR42_RS25525 0.27 +0.3
1,072,760 + RR42_RS25525 0.46 +0.5
1,072,760 + RR42_RS25525 0.46 -0.4
1,072,761 - RR42_RS25525 0.47 -0.3
1,072,807 + RR42_RS25525 0.51 +0.7
1,073,028 - RR42_RS25525 0.74 +0.7
1,073,111 + RR42_RS25525 0.82 -0.6
1,073,112 - RR42_RS25525 0.83 -1.0
1,073,243 + +0.2
1,073,243 + -1.6
1,073,243 + +0.7
1,073,244 - -0.2

Or see this region's nucleotide sequence