Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS21285

Experiment: D-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS21280 and RR42_RS21285 are separated by 510 nucleotidesRR42_RS21285 and RR42_RS21290 are separated by 35 nucleotidesRR42_RS21290 and RR42_RS21295 overlap by 4 nucleotides RR42_RS21280: RR42_RS21280 - L-lactate permease, at 99,858 to 101,600 _RS21280 RR42_RS21285: RR42_RS21285 - L-lactate dehydrogenase, LutB subunit (from data), at 102,111 to 103,541 _RS21285 RR42_RS21290: RR42_RS21290 - L-lactate dehydrogenase, LutC subunit (from data), at 103,577 to 104,281 _RS21290 RR42_RS21295: RR42_RS21295 - L-lactate dehydrogenase, LutA subunit (from data), at 104,278 to 105,066 _RS21295 Position (kb) 102 103 104Strain fitness (log2 ratio) -3 -2 -1 0 1at 101.315 kb on - strand, within RR42_RS21280at 101.329 kb on + strand, within RR42_RS21280at 101.377 kb on + strand, within RR42_RS21280at 101.428 kb on + strandat 101.568 kb on + strandat 101.569 kb on - strandat 101.569 kb on - strandat 101.578 kb on + strandat 101.701 kb on + strandat 101.701 kb on + strandat 101.701 kb on + strandat 101.701 kb on + strandat 102.070 kb on + strandat 102.071 kb on - strandat 102.112 kb on + strandat 102.167 kb on + strandat 102.534 kb on - strand, within RR42_RS21285at 102.534 kb on - strand, within RR42_RS21285at 102.565 kb on + strand, within RR42_RS21285at 102.565 kb on + strand, within RR42_RS21285at 102.565 kb on + strand, within RR42_RS21285at 102.565 kb on + strand, within RR42_RS21285at 102.637 kb on + strand, within RR42_RS21285at 102.733 kb on + strand, within RR42_RS21285at 102.734 kb on - strand, within RR42_RS21285at 103.013 kb on - strand, within RR42_RS21285at 103.028 kb on + strand, within RR42_RS21285at 103.123 kb on + strand, within RR42_RS21285at 103.124 kb on - strand, within RR42_RS21285at 103.171 kb on + strand, within RR42_RS21285at 103.574 kb on + strandat 103.574 kb on + strandat 103.575 kb on - strandat 103.575 kb on - strandat 103.616 kb on - strandat 103.695 kb on - strand, within RR42_RS21290at 103.695 kb on - strand, within RR42_RS21290at 103.779 kb on + strand, within RR42_RS21290at 103.889 kb on - strand, within RR42_RS21290at 104.175 kb on + strand, within RR42_RS21290at 104.175 kb on + strand, within RR42_RS21290at 104.175 kb on + strand, within RR42_RS21290at 104.176 kb on - strand, within RR42_RS21290at 104.240 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (N)
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101,315 - RR42_RS21280 0.84 -1.9
101,329 + RR42_RS21280 0.84 -2.9
101,377 + RR42_RS21280 0.87 -3.3
101,428 + -1.8
101,568 + -2.7
101,569 - -2.0
101,569 - -2.0
101,578 + -3.0
101,701 + -1.5
101,701 + -1.9
101,701 + -1.7
101,701 + -2.8
102,070 + -2.3
102,071 - +0.1
102,112 + -1.9
102,167 + -1.0
102,534 - RR42_RS21285 0.30 -2.2
102,534 - RR42_RS21285 0.30 -0.5
102,565 + RR42_RS21285 0.32 -0.9
102,565 + RR42_RS21285 0.32 -0.9
102,565 + RR42_RS21285 0.32 -1.7
102,565 + RR42_RS21285 0.32 -1.8
102,637 + RR42_RS21285 0.37 -2.6
102,733 + RR42_RS21285 0.43 +0.1
102,734 - RR42_RS21285 0.44 -1.7
103,013 - RR42_RS21285 0.63 -1.5
103,028 + RR42_RS21285 0.64 -0.2
103,123 + RR42_RS21285 0.71 -2.4
103,124 - RR42_RS21285 0.71 -1.1
103,171 + RR42_RS21285 0.74 -2.1
103,574 + -0.7
103,574 + -1.1
103,575 - -1.3
103,575 - -1.5
103,616 - -1.3
103,695 - RR42_RS21290 0.17 +0.9
103,695 - RR42_RS21290 0.17 -1.6
103,779 + RR42_RS21290 0.29 +0.1
103,889 - RR42_RS21290 0.44 -1.1
104,175 + RR42_RS21290 0.85 -1.2
104,175 + RR42_RS21290 0.85 -1.8
104,175 + RR42_RS21290 0.85 -2.5
104,176 - RR42_RS21290 0.85 -3.3
104,240 + +0.5

Or see this region's nucleotide sequence