Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21280 and RR42_RS21285 are separated by 510 nucleotides RR42_RS21285 and RR42_RS21290 are separated by 35 nucleotides RR42_RS21290 and RR42_RS21295 overlap by 4 nucleotides
RR42_RS21280: RR42_RS21280 - L-lactate permease, at 99,858 to 101,600
_RS21280
RR42_RS21285: RR42_RS21285 - L-lactate dehydrogenase, LutB subunit <small>(from data)</small>, at 102,111 to 103,541
_RS21285
RR42_RS21290: RR42_RS21290 - L-lactate dehydrogenase, LutC subunit <small>(from data)</small>, at 103,577 to 104,281
_RS21290
RR42_RS21295: RR42_RS21295 - L-lactate dehydrogenase, LutA subunit <small>(from data)</small>, at 104,278 to 105,066
_RS21295
Position (kb) 102
103
104 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 101.315 kb on - strand, within RR42_RS21280 at 101.329 kb on + strand, within RR42_RS21280 at 101.377 kb on + strand, within RR42_RS21280 at 101.428 kb on + strand at 101.568 kb on + strand at 101.569 kb on - strand at 101.569 kb on - strand at 101.578 kb on + strand at 101.701 kb on + strand at 101.701 kb on + strand at 101.701 kb on + strand at 101.701 kb on + strand at 102.070 kb on + strand at 102.071 kb on - strand at 102.112 kb on + strand at 102.167 kb on + strand at 102.534 kb on - strand, within RR42_RS21285 at 102.534 kb on - strand, within RR42_RS21285 at 102.565 kb on + strand, within RR42_RS21285 at 102.565 kb on + strand, within RR42_RS21285 at 102.565 kb on + strand, within RR42_RS21285 at 102.565 kb on + strand, within RR42_RS21285 at 102.637 kb on + strand, within RR42_RS21285 at 102.733 kb on + strand, within RR42_RS21285 at 102.734 kb on - strand, within RR42_RS21285 at 103.013 kb on - strand, within RR42_RS21285 at 103.028 kb on + strand, within RR42_RS21285 at 103.123 kb on + strand, within RR42_RS21285 at 103.124 kb on - strand, within RR42_RS21285 at 103.171 kb on + strand, within RR42_RS21285 at 103.574 kb on + strand at 103.574 kb on + strand at 103.575 kb on - strand at 103.575 kb on - strand at 103.616 kb on - strand at 103.695 kb on - strand, within RR42_RS21290 at 103.695 kb on - strand, within RR42_RS21290 at 103.779 kb on + strand, within RR42_RS21290 at 103.889 kb on - strand, within RR42_RS21290 at 104.175 kb on + strand, within RR42_RS21290 at 104.175 kb on + strand, within RR42_RS21290 at 104.175 kb on + strand, within RR42_RS21290 at 104.176 kb on - strand, within RR42_RS21290 at 104.240 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 101,315 - RR42_RS21280 0.84 -1.9 101,329 + RR42_RS21280 0.84 -2.9 101,377 + RR42_RS21280 0.87 -3.3 101,428 + -1.8 101,568 + -2.7 101,569 - -2.0 101,569 - -2.0 101,578 + -3.0 101,701 + -1.5 101,701 + -1.9 101,701 + -1.7 101,701 + -2.8 102,070 + -2.3 102,071 - +0.1 102,112 + -1.9 102,167 + -1.0 102,534 - RR42_RS21285 0.30 -2.2 102,534 - RR42_RS21285 0.30 -0.5 102,565 + RR42_RS21285 0.32 -0.9 102,565 + RR42_RS21285 0.32 -0.9 102,565 + RR42_RS21285 0.32 -1.7 102,565 + RR42_RS21285 0.32 -1.8 102,637 + RR42_RS21285 0.37 -2.6 102,733 + RR42_RS21285 0.43 +0.1 102,734 - RR42_RS21285 0.44 -1.7 103,013 - RR42_RS21285 0.63 -1.5 103,028 + RR42_RS21285 0.64 -0.2 103,123 + RR42_RS21285 0.71 -2.4 103,124 - RR42_RS21285 0.71 -1.1 103,171 + RR42_RS21285 0.74 -2.1 103,574 + -0.7 103,574 + -1.1 103,575 - -1.3 103,575 - -1.5 103,616 - -1.3 103,695 - RR42_RS21290 0.17 +0.9 103,695 - RR42_RS21290 0.17 -1.6 103,779 + RR42_RS21290 0.29 +0.1 103,889 - RR42_RS21290 0.44 -1.1 104,175 + RR42_RS21290 0.85 -1.2 104,175 + RR42_RS21290 0.85 -1.8 104,175 + RR42_RS21290 0.85 -2.5 104,176 - RR42_RS21290 0.85 -3.3 104,240 + +0.5
Or see this region's nucleotide sequence