Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17520 and RR42_RS17525 are separated by 142 nucleotides RR42_RS17525 and RR42_RS17530 are separated by 102 nucleotides RR42_RS17530 and RR42_RS17535 are separated by 116 nucleotides
RR42_RS17520: RR42_RS17520 - 2-hydroxychromene-2-carboxylate isomerase, at 3,791,713 to 3,792,318
_RS17520
RR42_RS17525: RR42_RS17525 - membrane protein, at 3,792,461 to 3,792,883
_RS17525
RR42_RS17530: RR42_RS17530 - gamma-glutamyl phosphate reductase, at 3,792,986 to 3,794,266
_RS17530
RR42_RS17535: RR42_RS17535 - DNA polymerase III subunit delta, at 3,794,383 to 3,795,423
_RS17535
Position (kb)
3792
3793
3794
3795 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3792.131 kb on + strand, within RR42_RS17520 at 3792.269 kb on + strand at 3792.269 kb on + strand at 3792.296 kb on + strand at 3792.296 kb on + strand at 3792.296 kb on + strand at 3792.296 kb on + strand at 3792.296 kb on + strand at 3792.297 kb on - strand at 3792.297 kb on - strand at 3792.297 kb on - strand at 3792.297 kb on - strand at 3792.297 kb on - strand at 3792.297 kb on - strand at 3792.299 kb on + strand at 3792.299 kb on + strand at 3792.299 kb on + strand at 3792.300 kb on - strand at 3792.391 kb on + strand at 3792.391 kb on + strand at 3792.392 kb on - strand at 3793.021 kb on - strand at 3793.021 kb on - strand at 3793.021 kb on - strand at 3793.108 kb on - strand at 3793.195 kb on - strand, within RR42_RS17530 at 3793.195 kb on - strand, within RR42_RS17530 at 3793.195 kb on - strand, within RR42_RS17530 at 3793.220 kb on - strand, within RR42_RS17530 at 3793.220 kb on - strand, within RR42_RS17530 at 3793.234 kb on - strand, within RR42_RS17530 at 3793.238 kb on - strand, within RR42_RS17530 at 3793.238 kb on - strand, within RR42_RS17530 at 3793.238 kb on - strand, within RR42_RS17530 at 3793.238 kb on - strand, within RR42_RS17530 at 3793.303 kb on - strand, within RR42_RS17530 at 3793.413 kb on + strand, within RR42_RS17530 at 3793.414 kb on - strand, within RR42_RS17530 at 3793.560 kb on + strand, within RR42_RS17530 at 3793.561 kb on - strand, within RR42_RS17530 at 3793.561 kb on - strand, within RR42_RS17530 at 3793.561 kb on - strand, within RR42_RS17530 at 3793.561 kb on - strand, within RR42_RS17530 at 3793.561 kb on - strand, within RR42_RS17530 at 3793.561 kb on - strand, within RR42_RS17530 at 3793.666 kb on - strand, within RR42_RS17530 at 3794.242 kb on - strand at 3794.242 kb on - strand at 3794.242 kb on - strand at 3794.242 kb on - strand at 3794.325 kb on - strand at 3794.325 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 3,792,131 + RR42_RS17520 0.69 +2.3 3,792,269 + -0.3 3,792,269 + +0.6 3,792,296 + -0.1 3,792,296 + -1.5 3,792,296 + -0.5 3,792,296 + -0.7 3,792,296 + -0.1 3,792,297 - +1.0 3,792,297 - +0.3 3,792,297 - +0.0 3,792,297 - -0.2 3,792,297 - +0.3 3,792,297 - +0.9 3,792,299 + -1.7 3,792,299 + +0.3 3,792,299 + +0.1 3,792,300 - -0.5 3,792,391 + -0.3 3,792,391 + -0.0 3,792,392 - +0.3 3,793,021 - -0.1 3,793,021 - +0.1 3,793,021 - +0.3 3,793,108 - +1.3 3,793,195 - RR42_RS17530 0.16 -2.2 3,793,195 - RR42_RS17530 0.16 +1.3 3,793,195 - RR42_RS17530 0.16 -0.6 3,793,220 - RR42_RS17530 0.18 +0.3 3,793,220 - RR42_RS17530 0.18 -1.0 3,793,234 - RR42_RS17530 0.19 +2.3 3,793,238 - RR42_RS17530 0.20 +0.0 3,793,238 - RR42_RS17530 0.20 -1.2 3,793,238 - RR42_RS17530 0.20 +0.1 3,793,238 - RR42_RS17530 0.20 +0.7 3,793,303 - RR42_RS17530 0.25 +0.4 3,793,413 + RR42_RS17530 0.33 -1.7 3,793,414 - RR42_RS17530 0.33 -1.9 3,793,560 + RR42_RS17530 0.45 -1.1 3,793,561 - RR42_RS17530 0.45 +0.1 3,793,561 - RR42_RS17530 0.45 -0.9 3,793,561 - RR42_RS17530 0.45 -0.3 3,793,561 - RR42_RS17530 0.45 -0.1 3,793,561 - RR42_RS17530 0.45 -0.5 3,793,561 - RR42_RS17530 0.45 -0.3 3,793,666 - RR42_RS17530 0.53 -0.3 3,794,242 - +0.4 3,794,242 - -0.9 3,794,242 - -0.5 3,794,242 - +0.4 3,794,325 - +0.2 3,794,325 - +0.4
Or see this region's nucleotide sequence