Experiment: PYE with sodium fluoride 7.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00483 and CCNA_00484 are separated by 89 nucleotides CCNA_00484 and CCNA_00485 are separated by 98 nucleotides CCNA_00485 and CCNA_00486 are separated by 92 nucleotides
CCNA_00483: CCNA_00483 - Zn-dependent hydrolase, glyoxalase II family, at 499,379 to 500,224
_00483
CCNA_00484: CCNA_00484 - hypothetical protein, at 500,314 to 501,015
_00484
CCNA_00485: CCNA_00485 - ribosomal large subunit pseudouridine synthase D, at 501,114 to 502,142
_00485
CCNA_00486: CCNA_00486 - TonB-dependent receptor, at 502,235 to 504,724
_00486
Position (kb) 501
502
503 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 500.140 kb on + strand at 500.216 kb on - strand at 500.228 kb on + strand at 500.270 kb on + strand at 500.343 kb on + strand at 500.363 kb on + strand at 500.363 kb on + strand at 500.364 kb on + strand at 500.371 kb on - strand at 500.374 kb on + strand at 500.434 kb on + strand, within CCNA_00484 at 500.441 kb on + strand at 500.455 kb on + strand, within CCNA_00484 at 500.503 kb on + strand, within CCNA_00484 at 500.521 kb on + strand, within CCNA_00484 at 500.521 kb on + strand, within CCNA_00484 at 500.529 kb on - strand, within CCNA_00484 at 500.553 kb on - strand, within CCNA_00484 at 500.614 kb on + strand, within CCNA_00484 at 500.716 kb on + strand, within CCNA_00484 at 500.724 kb on - strand, within CCNA_00484 at 500.754 kb on - strand, within CCNA_00484 at 500.777 kb on + strand, within CCNA_00484 at 500.825 kb on - strand, within CCNA_00484 at 500.825 kb on - strand, within CCNA_00484 at 500.849 kb on + strand, within CCNA_00484 at 500.856 kb on - strand, within CCNA_00484 at 500.902 kb on + strand, within CCNA_00484 at 500.902 kb on + strand, within CCNA_00484 at 500.910 kb on - strand, within CCNA_00484 at 500.910 kb on - strand, within CCNA_00484 at 500.939 kb on - strand, within CCNA_00484 at 500.979 kb on - strand at 501.002 kb on + strand at 501.010 kb on - strand at 501.046 kb on - strand at 501.050 kb on + strand at 501.054 kb on - strand at 501.059 kb on - strand at 501.073 kb on - strand at 501.096 kb on + strand at 501.103 kb on - strand at 501.103 kb on - strand at 501.103 kb on - strand at 501.104 kb on - strand at 501.120 kb on - strand at 501.134 kb on + strand at 501.134 kb on + strand at 501.134 kb on + strand at 501.134 kb on + strand at 501.134 kb on + strand at 501.134 kb on + strand at 501.134 kb on + strand at 501.134 kb on + strand at 501.142 kb on - strand at 501.142 kb on - strand at 501.142 kb on - strand at 501.142 kb on - strand at 501.142 kb on - strand at 501.142 kb on - strand at 501.142 kb on - strand at 501.142 kb on - strand at 501.142 kb on - strand at 501.142 kb on - strand at 501.142 kb on - strand at 501.161 kb on + strand at 501.179 kb on + strand at 501.207 kb on + strand at 501.207 kb on + strand at 501.207 kb on + strand at 501.207 kb on + strand at 501.208 kb on - strand at 501.215 kb on + strand at 501.215 kb on - strand at 501.217 kb on + strand, within CCNA_00485 at 501.234 kb on + strand, within CCNA_00485 at 501.263 kb on + strand, within CCNA_00485 at 501.305 kb on + strand, within CCNA_00485 at 501.305 kb on + strand, within CCNA_00485 at 501.305 kb on + strand, within CCNA_00485 at 501.345 kb on + strand, within CCNA_00485 at 501.345 kb on + strand, within CCNA_00485 at 501.345 kb on + strand, within CCNA_00485 at 501.409 kb on + strand, within CCNA_00485 at 501.419 kb on - strand, within CCNA_00485 at 501.427 kb on + strand, within CCNA_00485 at 501.427 kb on + strand, within CCNA_00485 at 501.429 kb on - strand, within CCNA_00485 at 501.430 kb on - strand, within CCNA_00485 at 501.435 kb on - strand, within CCNA_00485 at 501.435 kb on - strand, within CCNA_00485 at 501.435 kb on - strand, within CCNA_00485 at 501.435 kb on - strand, within CCNA_00485 at 501.440 kb on - strand, within CCNA_00485 at 501.440 kb on - strand, within CCNA_00485 at 501.488 kb on + strand, within CCNA_00485 at 501.496 kb on - strand, within CCNA_00485 at 501.511 kb on + strand, within CCNA_00485 at 501.513 kb on - strand, within CCNA_00485 at 501.549 kb on + strand, within CCNA_00485 at 501.549 kb on + strand, within CCNA_00485 at 501.549 kb on + strand, within CCNA_00485 at 501.549 kb on + strand, within CCNA_00485 at 501.557 kb on - strand, within CCNA_00485 at 501.557 kb on - strand, within CCNA_00485 at 501.557 kb on - strand, within CCNA_00485 at 501.557 kb on - strand, within CCNA_00485 at 501.559 kb on + strand, within CCNA_00485 at 501.559 kb on + strand, within CCNA_00485 at 501.559 kb on + strand, within CCNA_00485 at 501.559 kb on + strand, within CCNA_00485 at 501.559 kb on + strand, within CCNA_00485 at 501.559 kb on + strand, within CCNA_00485 at 501.559 kb on + strand, within CCNA_00485 at 501.559 kb on + strand, within CCNA_00485 at 501.559 kb on + strand, within CCNA_00485 at 501.559 kb on + strand, within CCNA_00485 at 501.567 kb on - strand, within CCNA_00485 at 501.567 kb on - strand, within CCNA_00485 at 501.567 kb on - strand, within CCNA_00485 at 501.567 kb on - strand, within CCNA_00485 at 501.591 kb on - strand, within CCNA_00485 at 501.591 kb on - strand, within CCNA_00485 at 501.601 kb on - strand, within CCNA_00485 at 501.606 kb on + strand, within CCNA_00485 at 501.641 kb on + strand, within CCNA_00485 at 501.641 kb on + strand, within CCNA_00485 at 501.649 kb on - strand, within CCNA_00485 at 501.649 kb on - strand, within CCNA_00485 at 501.673 kb on + strand, within CCNA_00485 at 501.673 kb on + strand, within CCNA_00485 at 501.681 kb on - strand, within CCNA_00485 at 501.681 kb on - strand, within CCNA_00485 at 501.681 kb on - strand, within CCNA_00485 at 501.715 kb on + strand, within CCNA_00485 at 501.731 kb on - strand, within CCNA_00485 at 501.733 kb on + strand, within CCNA_00485 at 501.733 kb on + strand, within CCNA_00485 at 501.741 kb on - strand, within CCNA_00485 at 501.741 kb on - strand, within CCNA_00485 at 501.741 kb on - strand, within CCNA_00485 at 501.741 kb on - strand, within CCNA_00485 at 501.741 kb on - strand, within CCNA_00485 at 501.741 kb on - strand, within CCNA_00485 at 501.748 kb on + strand, within CCNA_00485 at 501.790 kb on + strand, within CCNA_00485 at 501.795 kb on + strand, within CCNA_00485 at 501.795 kb on + strand, within CCNA_00485 at 501.798 kb on - strand, within CCNA_00485 at 501.802 kb on + strand, within CCNA_00485 at 501.817 kb on + strand, within CCNA_00485 at 501.817 kb on - strand, within CCNA_00485 at 501.837 kb on + strand, within CCNA_00485 at 501.881 kb on + strand, within CCNA_00485 at 501.887 kb on + strand, within CCNA_00485 at 501.891 kb on + strand, within CCNA_00485 at 501.929 kb on - strand, within CCNA_00485 at 501.939 kb on + strand, within CCNA_00485 at 501.945 kb on + strand, within CCNA_00485 at 501.963 kb on + strand, within CCNA_00485 at 501.970 kb on + strand, within CCNA_00485 at 501.972 kb on - strand, within CCNA_00485 at 501.994 kb on + strand, within CCNA_00485 at 502.015 kb on + strand, within CCNA_00485 at 502.029 kb on - strand, within CCNA_00485 at 502.052 kb on + strand at 502.052 kb on + strand at 502.052 kb on + strand at 502.052 kb on + strand at 502.053 kb on + strand at 502.060 kb on - strand at 502.060 kb on - strand at 502.060 kb on - strand at 502.060 kb on - strand at 502.065 kb on + strand at 502.065 kb on - strand at 502.065 kb on - strand at 502.072 kb on + strand at 502.072 kb on + strand at 502.125 kb on + strand at 502.150 kb on + strand at 502.165 kb on - strand at 502.192 kb on + strand at 502.200 kb on - strand at 502.239 kb on - strand at 502.252 kb on - strand at 502.261 kb on + strand at 502.264 kb on - strand at 502.264 kb on - strand at 502.269 kb on - strand at 502.275 kb on + strand at 502.282 kb on - strand at 502.282 kb on - strand at 502.282 kb on - strand at 502.282 kb on - strand at 502.282 kb on - strand at 502.282 kb on - strand at 502.283 kb on - strand at 502.283 kb on - strand at 502.295 kb on + strand at 502.303 kb on - strand at 502.304 kb on - strand at 502.319 kb on - strand at 502.344 kb on + strand at 502.344 kb on + strand at 502.344 kb on + strand at 502.354 kb on + strand at 502.362 kb on - strand at 502.362 kb on - strand at 502.366 kb on + strand at 502.376 kb on + strand at 502.446 kb on - strand at 502.474 kb on + strand at 502.502 kb on + strand, within CCNA_00486 at 502.538 kb on - strand, within CCNA_00486 at 502.558 kb on - strand at 502.574 kb on + strand, within CCNA_00486 at 502.574 kb on + strand, within CCNA_00486 at 502.706 kb on + strand, within CCNA_00486 at 502.711 kb on + strand, within CCNA_00486 at 502.714 kb on - strand, within CCNA_00486 at 502.719 kb on - strand, within CCNA_00486 at 502.738 kb on - strand, within CCNA_00486 at 502.743 kb on - strand, within CCNA_00486 at 502.748 kb on - strand, within CCNA_00486 at 502.754 kb on + strand, within CCNA_00486 at 502.755 kb on + strand, within CCNA_00486 at 502.755 kb on + strand, within CCNA_00486 at 502.755 kb on + strand, within CCNA_00486 at 502.819 kb on - strand, within CCNA_00486 at 502.919 kb on - strand, within CCNA_00486 at 502.954 kb on - strand, within CCNA_00486 at 502.954 kb on - strand, within CCNA_00486 at 502.956 kb on - strand, within CCNA_00486 at 502.974 kb on + strand, within CCNA_00486 at 502.976 kb on - strand, within CCNA_00486 at 502.979 kb on + strand, within CCNA_00486 at 502.982 kb on - strand, within CCNA_00486 at 502.982 kb on - strand, within CCNA_00486 at 502.984 kb on + strand, within CCNA_00486 at 503.001 kb on - strand, within CCNA_00486 at 503.031 kb on + strand, within CCNA_00486 at 503.037 kb on + strand, within CCNA_00486 at 503.107 kb on + strand, within CCNA_00486 at 503.110 kb on - strand, within CCNA_00486 at 503.115 kb on - strand, within CCNA_00486 at 503.120 kb on + strand, within CCNA_00486 at 503.129 kb on - strand, within CCNA_00486 at 503.140 kb on + strand, within CCNA_00486
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with sodium fluoride 7.5 mM remove 500,140 + -0.6 500,216 - +0.4 500,228 + -0.9 500,270 + -1.4 500,343 + -0.1 500,363 + -0.5 500,363 + +0.1 500,364 + -1.1 500,371 - -1.2 500,374 + -2.5 500,434 + CCNA_00484 0.17 -0.4 500,441 + +0.4 500,455 + CCNA_00484 0.20 -2.7 500,503 + CCNA_00484 0.27 -0.5 500,521 + CCNA_00484 0.29 -1.6 500,521 + CCNA_00484 0.29 +0.3 500,529 - CCNA_00484 0.31 +0.7 500,553 - CCNA_00484 0.34 +1.0 500,614 + CCNA_00484 0.43 -0.7 500,716 + CCNA_00484 0.57 -0.3 500,724 - CCNA_00484 0.58 +0.1 500,754 - CCNA_00484 0.63 +0.2 500,777 + CCNA_00484 0.66 +1.4 500,825 - CCNA_00484 0.73 +0.3 500,825 - CCNA_00484 0.73 +0.9 500,849 + CCNA_00484 0.76 -1.2 500,856 - CCNA_00484 0.77 -0.0 500,902 + CCNA_00484 0.84 -1.5 500,902 + CCNA_00484 0.84 -1.3 500,910 - CCNA_00484 0.85 -0.7 500,910 - CCNA_00484 0.85 +0.8 500,939 - CCNA_00484 0.89 +0.3 500,979 - +0.2 501,002 + -1.0 501,010 - -0.8 501,046 - -0.2 501,050 + -0.1 501,054 - +0.1 501,059 - +0.2 501,073 - -0.6 501,096 + -1.0 501,103 - -0.6 501,103 - +0.1 501,103 - -0.8 501,104 - -1.0 501,120 - -0.5 501,134 + -3.5 501,134 + -0.7 501,134 + -2.5 501,134 + -2.0 501,134 + -2.8 501,134 + -1.1 501,134 + -1.4 501,134 + -0.8 501,142 - -0.2 501,142 - -0.2 501,142 - -0.8 501,142 - -0.1 501,142 - -0.4 501,142 - -1.5 501,142 - -0.5 501,142 - -1.4 501,142 - -0.2 501,142 - -0.6 501,142 - -0.8 501,161 + -1.6 501,179 + -2.1 501,207 + -0.9 501,207 + -2.1 501,207 + -1.1 501,207 + +0.9 501,208 - -1.0 501,215 + -2.5 501,215 - -2.3 501,217 + CCNA_00485 0.10 -0.4 501,234 + CCNA_00485 0.12 -1.0 501,263 + CCNA_00485 0.14 -3.0 501,305 + CCNA_00485 0.19 -1.6 501,305 + CCNA_00485 0.19 -2.2 501,305 + CCNA_00485 0.19 -3.0 501,345 + CCNA_00485 0.22 -1.4 501,345 + CCNA_00485 0.22 -2.7 501,345 + CCNA_00485 0.22 -2.4 501,409 + CCNA_00485 0.29 -1.0 501,419 - CCNA_00485 0.30 -1.2 501,427 + CCNA_00485 0.30 -1.7 501,427 + CCNA_00485 0.30 -1.6 501,429 - CCNA_00485 0.31 -1.4 501,430 - CCNA_00485 0.31 -1.7 501,435 - CCNA_00485 0.31 -1.0 501,435 - CCNA_00485 0.31 -3.8 501,435 - CCNA_00485 0.31 +2.0 501,435 - CCNA_00485 0.31 -1.8 501,440 - CCNA_00485 0.32 -2.6 501,440 - CCNA_00485 0.32 -3.1 501,488 + CCNA_00485 0.36 -0.9 501,496 - CCNA_00485 0.37 -1.0 501,511 + CCNA_00485 0.39 -0.4 501,513 - CCNA_00485 0.39 -2.1 501,549 + CCNA_00485 0.42 -1.5 501,549 + CCNA_00485 0.42 -0.3 501,549 + CCNA_00485 0.42 -1.5 501,549 + CCNA_00485 0.42 -3.0 501,557 - CCNA_00485 0.43 -3.0 501,557 - CCNA_00485 0.43 -0.5 501,557 - CCNA_00485 0.43 -0.1 501,557 - CCNA_00485 0.43 -2.4 501,559 + CCNA_00485 0.43 -2.0 501,559 + CCNA_00485 0.43 -2.0 501,559 + CCNA_00485 0.43 -1.2 501,559 + CCNA_00485 0.43 -1.3 501,559 + CCNA_00485 0.43 -2.9 501,559 + CCNA_00485 0.43 +1.7 501,559 + CCNA_00485 0.43 +0.6 501,559 + CCNA_00485 0.43 -3.8 501,559 + CCNA_00485 0.43 +0.1 501,559 + CCNA_00485 0.43 +0.3 501,567 - CCNA_00485 0.44 -1.6 501,567 - CCNA_00485 0.44 -1.1 501,567 - CCNA_00485 0.44 -2.1 501,567 - CCNA_00485 0.44 -1.0 501,591 - CCNA_00485 0.46 -0.3 501,591 - CCNA_00485 0.46 -0.4 501,601 - CCNA_00485 0.47 -1.1 501,606 + CCNA_00485 0.48 -1.5 501,641 + CCNA_00485 0.51 -2.1 501,641 + CCNA_00485 0.51 -2.1 501,649 - CCNA_00485 0.52 -2.0 501,649 - CCNA_00485 0.52 -1.6 501,673 + CCNA_00485 0.54 -0.8 501,673 + CCNA_00485 0.54 -0.7 501,681 - CCNA_00485 0.55 -2.1 501,681 - CCNA_00485 0.55 +0.6 501,681 - CCNA_00485 0.55 -3.0 501,715 + CCNA_00485 0.58 -2.4 501,731 - CCNA_00485 0.60 -2.6 501,733 + CCNA_00485 0.60 -1.5 501,733 + CCNA_00485 0.60 -0.1 501,741 - CCNA_00485 0.61 -0.6 501,741 - CCNA_00485 0.61 -4.1 501,741 - CCNA_00485 0.61 -1.7 501,741 - CCNA_00485 0.61 -1.4 501,741 - CCNA_00485 0.61 -3.6 501,741 - CCNA_00485 0.61 -1.7 501,748 + CCNA_00485 0.62 -1.4 501,790 + CCNA_00485 0.66 -1.0 501,795 + CCNA_00485 0.66 -1.9 501,795 + CCNA_00485 0.66 -4.1 501,798 - CCNA_00485 0.66 -3.3 501,802 + CCNA_00485 0.67 -2.8 501,817 + CCNA_00485 0.68 -1.8 501,817 - CCNA_00485 0.68 -2.1 501,837 + CCNA_00485 0.70 -1.1 501,881 + CCNA_00485 0.75 -1.7 501,887 + CCNA_00485 0.75 +0.1 501,891 + CCNA_00485 0.76 -2.0 501,929 - CCNA_00485 0.79 -2.0 501,939 + CCNA_00485 0.80 -2.5 501,945 + CCNA_00485 0.81 -1.6 501,963 + CCNA_00485 0.83 -1.7 501,970 + CCNA_00485 0.83 -1.6 501,972 - CCNA_00485 0.83 -2.7 501,994 + CCNA_00485 0.86 -0.3 502,015 + CCNA_00485 0.88 -0.3 502,029 - CCNA_00485 0.89 -1.9 502,052 + -1.0 502,052 + -2.4 502,052 + -0.6 502,052 + -1.1 502,053 + -1.3 502,060 - -2.0 502,060 - -1.9 502,060 - -2.1 502,060 - -1.7 502,065 + +0.6 502,065 - +0.1 502,065 - -0.1 502,072 + +0.1 502,072 + -0.2 502,125 + -1.9 502,150 + -0.0 502,165 - -0.1 502,192 + -2.1 502,200 - -2.4 502,239 - +0.2 502,252 - -0.3 502,261 + -0.0 502,264 - -0.1 502,264 - -1.9 502,269 - +0.3 502,275 + -1.1 502,282 - +1.1 502,282 - -0.1 502,282 - -1.3 502,282 - -2.4 502,282 - +0.7 502,282 - -1.0 502,283 - -0.0 502,283 - -0.7 502,295 + +0.1 502,303 - +1.6 502,304 - -0.9 502,319 - -1.1 502,344 + -0.2 502,344 + -0.4 502,344 + -3.1 502,354 + +0.7 502,362 - -0.9 502,362 - -0.8 502,366 + -0.1 502,376 + -0.0 502,446 - -1.1 502,474 + +0.9 502,502 + CCNA_00486 0.11 +0.5 502,538 - CCNA_00486 0.12 -1.5 502,558 - +1.4 502,574 + CCNA_00486 0.14 +0.5 502,574 + CCNA_00486 0.14 -0.4 502,706 + CCNA_00486 0.19 -0.4 502,711 + CCNA_00486 0.19 -0.5 502,714 - CCNA_00486 0.19 -2.0 502,719 - CCNA_00486 0.19 -0.3 502,738 - CCNA_00486 0.20 -0.4 502,743 - CCNA_00486 0.20 -1.2 502,748 - CCNA_00486 0.21 -0.1 502,754 + CCNA_00486 0.21 -0.9 502,755 + CCNA_00486 0.21 -1.5 502,755 + CCNA_00486 0.21 -0.2 502,755 + CCNA_00486 0.21 -0.7 502,819 - CCNA_00486 0.23 +0.9 502,919 - CCNA_00486 0.27 +1.0 502,954 - CCNA_00486 0.29 -0.8 502,954 - CCNA_00486 0.29 +0.7 502,956 - CCNA_00486 0.29 +0.4 502,974 + CCNA_00486 0.30 -3.0 502,976 - CCNA_00486 0.30 +0.0 502,979 + CCNA_00486 0.30 +0.3 502,982 - CCNA_00486 0.30 -0.1 502,982 - CCNA_00486 0.30 +1.4 502,984 + CCNA_00486 0.30 +0.0 503,001 - CCNA_00486 0.31 -0.3 503,031 + CCNA_00486 0.32 -2.4 503,037 + CCNA_00486 0.32 -0.3 503,107 + CCNA_00486 0.35 +0.4 503,110 - CCNA_00486 0.35 +0.4 503,115 - CCNA_00486 0.35 +1.1 503,120 + CCNA_00486 0.36 +0.8 503,129 - CCNA_00486 0.36 -0.7 503,140 + CCNA_00486 0.36 -1.7
Or see this region's nucleotide sequence