Experiment: Recovery broth with Carbenicillin 5e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13065 and BBR_RS13070 are separated by 244 nucleotides BBR_RS13070 and BBR_RS13075 are separated by 43 nucleotides BBR_RS13075 and BBR_RS13080 overlap by 8 nucleotides
BBR_RS13065: BBR_RS13065 - amino acid ABC transporter ATP-binding protein, at 704,076 to 704,906
_RS13065
BBR_RS13070: BBR_RS13070 - glutamine--fructose-6-phosphate transaminase (isomerizing), at 705,151 to 707,043
_RS13070
BBR_RS13075: BBR_RS13075 - 23S rRNA pseudouridylate synthase, at 707,087 to 707,821
_RS13075
BBR_RS13080: BBR_RS13080 - LacI family DNA-binding transcriptional regulator, at 707,814 to 708,800
_RS13080
Position (kb)
705
706
707
708 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 704.157 kb on + strand at 704.165 kb on + strand, within BBR_RS13065 at 704.180 kb on - strand, within BBR_RS13065 at 704.192 kb on + strand, within BBR_RS13065 at 704.192 kb on + strand, within BBR_RS13065 at 704.200 kb on - strand, within BBR_RS13065 at 704.219 kb on + strand, within BBR_RS13065 at 704.219 kb on + strand, within BBR_RS13065 at 704.219 kb on + strand, within BBR_RS13065 at 704.232 kb on - strand, within BBR_RS13065 at 704.264 kb on + strand, within BBR_RS13065 at 704.271 kb on + strand, within BBR_RS13065 at 704.271 kb on + strand, within BBR_RS13065 at 704.373 kb on + strand, within BBR_RS13065 at 704.381 kb on - strand, within BBR_RS13065 at 704.383 kb on + strand, within BBR_RS13065 at 704.424 kb on + strand, within BBR_RS13065 at 704.430 kb on + strand, within BBR_RS13065 at 704.436 kb on - strand, within BBR_RS13065 at 704.504 kb on + strand, within BBR_RS13065 at 704.512 kb on - strand, within BBR_RS13065 at 704.518 kb on - strand, within BBR_RS13065 at 704.534 kb on + strand, within BBR_RS13065 at 704.562 kb on - strand, within BBR_RS13065 at 704.567 kb on - strand, within BBR_RS13065 at 704.576 kb on + strand, within BBR_RS13065 at 704.605 kb on + strand, within BBR_RS13065 at 704.709 kb on + strand, within BBR_RS13065 at 704.711 kb on + strand, within BBR_RS13065 at 704.716 kb on + strand, within BBR_RS13065 at 704.719 kb on - strand, within BBR_RS13065 at 704.719 kb on - strand, within BBR_RS13065 at 704.724 kb on - strand, within BBR_RS13065 at 704.741 kb on + strand, within BBR_RS13065 at 704.741 kb on + strand, within BBR_RS13065 at 704.741 kb on + strand, within BBR_RS13065 at 704.748 kb on + strand, within BBR_RS13065 at 704.750 kb on - strand, within BBR_RS13065 at 704.754 kb on + strand, within BBR_RS13065 at 704.784 kb on - strand, within BBR_RS13065 at 704.898 kb on + strand at 704.958 kb on + strand at 704.958 kb on + strand at 704.969 kb on + strand at 705.022 kb on + strand at 705.025 kb on + strand at 706.065 kb on + strand, within BBR_RS13070 at 707.058 kb on - strand at 707.098 kb on + strand at 707.098 kb on + strand at 707.098 kb on + strand at 707.108 kb on + strand at 707.123 kb on - strand at 707.188 kb on - strand, within BBR_RS13075 at 707.202 kb on + strand, within BBR_RS13075 at 707.202 kb on - strand, within BBR_RS13075 at 707.210 kb on - strand, within BBR_RS13075 at 707.225 kb on + strand, within BBR_RS13075 at 707.263 kb on + strand, within BBR_RS13075 at 707.266 kb on - strand, within BBR_RS13075 at 707.322 kb on - strand, within BBR_RS13075 at 707.407 kb on - strand, within BBR_RS13075 at 707.439 kb on + strand, within BBR_RS13075 at 707.538 kb on + strand, within BBR_RS13075 at 707.571 kb on - strand, within BBR_RS13075 at 707.573 kb on + strand, within BBR_RS13075 at 707.574 kb on + strand, within BBR_RS13075 at 707.582 kb on - strand, within BBR_RS13075 at 707.604 kb on + strand, within BBR_RS13075 at 707.653 kb on - strand, within BBR_RS13075 at 707.655 kb on - strand, within BBR_RS13075 at 707.660 kb on + strand, within BBR_RS13075 at 707.668 kb on - strand, within BBR_RS13075 at 707.668 kb on - strand, within BBR_RS13075 at 707.775 kb on - strand at 707.777 kb on - strand at 707.807 kb on + strand at 707.807 kb on - strand at 707.817 kb on + strand at 707.857 kb on - strand at 707.893 kb on - strand at 707.919 kb on - strand, within BBR_RS13080 at 707.919 kb on - strand, within BBR_RS13080 at 707.957 kb on + strand, within BBR_RS13080 at 707.983 kb on + strand, within BBR_RS13080
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Carbenicillin 5e-04 mM remove 704,157 + +0.4 704,165 + BBR_RS13065 0.11 +0.5 704,180 - BBR_RS13065 0.13 +1.0 704,192 + BBR_RS13065 0.14 -0.3 704,192 + BBR_RS13065 0.14 +0.2 704,200 - BBR_RS13065 0.15 -0.2 704,219 + BBR_RS13065 0.17 +0.3 704,219 + BBR_RS13065 0.17 +0.4 704,219 + BBR_RS13065 0.17 +0.1 704,232 - BBR_RS13065 0.19 +0.6 704,264 + BBR_RS13065 0.23 -1.1 704,271 + BBR_RS13065 0.23 -0.0 704,271 + BBR_RS13065 0.23 +0.1 704,373 + BBR_RS13065 0.36 +0.1 704,381 - BBR_RS13065 0.37 +1.0 704,383 + BBR_RS13065 0.37 +0.4 704,424 + BBR_RS13065 0.42 -0.5 704,430 + BBR_RS13065 0.43 -0.3 704,436 - BBR_RS13065 0.43 +1.0 704,504 + BBR_RS13065 0.52 -0.9 704,512 - BBR_RS13065 0.52 -0.5 704,518 - BBR_RS13065 0.53 +0.6 704,534 + BBR_RS13065 0.55 +0.0 704,562 - BBR_RS13065 0.58 -0.2 704,567 - BBR_RS13065 0.59 -1.8 704,576 + BBR_RS13065 0.60 +0.2 704,605 + BBR_RS13065 0.64 +0.2 704,709 + BBR_RS13065 0.76 +0.6 704,711 + BBR_RS13065 0.76 -0.1 704,716 + BBR_RS13065 0.77 +0.0 704,719 - BBR_RS13065 0.77 +0.3 704,719 - BBR_RS13065 0.77 -0.4 704,724 - BBR_RS13065 0.78 +0.2 704,741 + BBR_RS13065 0.80 +0.5 704,741 + BBR_RS13065 0.80 +0.8 704,741 + BBR_RS13065 0.80 +0.7 704,748 + BBR_RS13065 0.81 -0.0 704,750 - BBR_RS13065 0.81 -0.1 704,754 + BBR_RS13065 0.82 -1.1 704,784 - BBR_RS13065 0.85 +0.4 704,898 + +0.4 704,958 + -0.1 704,958 + -0.1 704,969 + -1.4 705,022 + -0.8 705,025 + +0.1 706,065 + BBR_RS13070 0.48 -3.3 707,058 - +1.4 707,098 + +0.4 707,098 + -1.4 707,098 + +0.1 707,108 + -1.0 707,123 - +0.3 707,188 - BBR_RS13075 0.14 +0.7 707,202 + BBR_RS13075 0.16 +1.0 707,202 - BBR_RS13075 0.16 -0.2 707,210 - BBR_RS13075 0.17 +4.0 707,225 + BBR_RS13075 0.19 +0.3 707,263 + BBR_RS13075 0.24 +0.6 707,266 - BBR_RS13075 0.24 +1.2 707,322 - BBR_RS13075 0.32 +0.1 707,407 - BBR_RS13075 0.44 +0.4 707,439 + BBR_RS13075 0.48 -1.3 707,538 + BBR_RS13075 0.61 +0.7 707,571 - BBR_RS13075 0.66 +0.7 707,573 + BBR_RS13075 0.66 +0.7 707,574 + BBR_RS13075 0.66 -0.6 707,582 - BBR_RS13075 0.67 +0.6 707,604 + BBR_RS13075 0.70 -0.3 707,653 - BBR_RS13075 0.77 +0.2 707,655 - BBR_RS13075 0.77 +0.2 707,660 + BBR_RS13075 0.78 -0.2 707,668 - BBR_RS13075 0.79 -1.2 707,668 - BBR_RS13075 0.79 +0.4 707,775 - -0.1 707,777 - +0.5 707,807 + +0.2 707,807 - -0.7 707,817 + -0.3 707,857 - +0.4 707,893 - -0.2 707,919 - BBR_RS13080 0.11 -0.4 707,919 - BBR_RS13080 0.11 +0.4 707,957 + BBR_RS13080 0.14 -0.0 707,983 + BBR_RS13080 0.17 +0.7
Or see this region's nucleotide sequence