Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0466 and Rru_A0467 are separated by 121 nucleotides Rru_A0467 and Rru_A0468 are separated by 127 nucleotides Rru_A0468 and Rru_A0469 are separated by 126 nucleotides
Rru_A0466: Rru_A0466 - tRNA isopentenyltransferase (NCBI), at 546,394 to 547,374
_A0466
Rru_A0467: Rru_A0467 - Acetolactate synthase, large subunit, biosynthetic type (NCBI), at 547,496 to 549,256
_A0467
Rru_A0468: Rru_A0468 - Acetolactate synthase, small subunit (NCBI), at 549,384 to 549,911
_A0468
Rru_A0469: Rru_A0469 - Ketol-acid reductoisomerase (NCBI), at 550,038 to 551,057
_A0469
Position (kb) 547
548
549
550 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 547.601 kb on + strand at 547.682 kb on + strand, within Rru_A0467 at 547.682 kb on + strand, within Rru_A0467 at 547.682 kb on + strand, within Rru_A0467 at 547.889 kb on + strand, within Rru_A0467 at 547.889 kb on + strand, within Rru_A0467 at 547.890 kb on - strand, within Rru_A0467 at 547.890 kb on - strand, within Rru_A0467 at 547.890 kb on - strand, within Rru_A0467 at 548.015 kb on + strand, within Rru_A0467 at 548.015 kb on + strand, within Rru_A0467 at 548.016 kb on - strand, within Rru_A0467 at 548.048 kb on + strand, within Rru_A0467 at 548.048 kb on + strand, within Rru_A0467 at 548.129 kb on + strand, within Rru_A0467 at 548.240 kb on + strand, within Rru_A0467 at 548.240 kb on + strand, within Rru_A0467 at 548.241 kb on - strand, within Rru_A0467 at 548.261 kb on + strand, within Rru_A0467 at 548.262 kb on - strand, within Rru_A0467 at 548.519 kb on + strand, within Rru_A0467 at 548.519 kb on + strand, within Rru_A0467 at 548.520 kb on - strand, within Rru_A0467 at 548.520 kb on - strand, within Rru_A0467 at 548.595 kb on - strand, within Rru_A0467 at 548.615 kb on - strand, within Rru_A0467 at 548.615 kb on - strand, within Rru_A0467 at 548.627 kb on + strand, within Rru_A0467 at 548.627 kb on + strand, within Rru_A0467 at 548.627 kb on + strand, within Rru_A0467 at 548.645 kb on + strand, within Rru_A0467 at 548.645 kb on + strand, within Rru_A0467 at 548.645 kb on + strand, within Rru_A0467 at 548.717 kb on + strand, within Rru_A0467 at 548.718 kb on - strand, within Rru_A0467 at 548.894 kb on + strand, within Rru_A0467 at 548.894 kb on + strand, within Rru_A0467 at 548.895 kb on - strand, within Rru_A0467 at 548.927 kb on - strand, within Rru_A0467 at 548.933 kb on + strand, within Rru_A0467 at 548.993 kb on + strand, within Rru_A0467 at 548.994 kb on - strand, within Rru_A0467 at 548.994 kb on - strand, within Rru_A0467 at 548.994 kb on - strand, within Rru_A0467 at 549.254 kb on + strand at 549.428 kb on + strand at 549.429 kb on - strand at 549.501 kb on + strand, within Rru_A0468 at 549.503 kb on + strand, within Rru_A0468 at 549.503 kb on + strand, within Rru_A0468 at 549.503 kb on + strand, within Rru_A0468 at 549.504 kb on - strand, within Rru_A0468 at 549.909 kb on + strand at 549.910 kb on - strand at 549.910 kb on - strand at 549.918 kb on - strand at 549.960 kb on - strand at 550.048 kb on - strand at 550.048 kb on - strand at 550.107 kb on + strand at 550.136 kb on + strand at 550.137 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 547,601 + -0.9 547,682 + Rru_A0467 0.11 -2.0 547,682 + Rru_A0467 0.11 -0.4 547,682 + Rru_A0467 0.11 -3.4 547,889 + Rru_A0467 0.22 -5.9 547,889 + Rru_A0467 0.22 -1.3 547,890 - Rru_A0467 0.22 -0.9 547,890 - Rru_A0467 0.22 -1.2 547,890 - Rru_A0467 0.22 -3.5 548,015 + Rru_A0467 0.29 -4.3 548,015 + Rru_A0467 0.29 -2.3 548,016 - Rru_A0467 0.30 -5.0 548,048 + Rru_A0467 0.31 -3.1 548,048 + Rru_A0467 0.31 -4.0 548,129 + Rru_A0467 0.36 -1.7 548,240 + Rru_A0467 0.42 -2.3 548,240 + Rru_A0467 0.42 -0.8 548,241 - Rru_A0467 0.42 -3.5 548,261 + Rru_A0467 0.43 -1.6 548,262 - Rru_A0467 0.43 -4.3 548,519 + Rru_A0467 0.58 -2.6 548,519 + Rru_A0467 0.58 -3.0 548,520 - Rru_A0467 0.58 -3.6 548,520 - Rru_A0467 0.58 -5.2 548,595 - Rru_A0467 0.62 -1.9 548,615 - Rru_A0467 0.64 -4.4 548,615 - Rru_A0467 0.64 -2.8 548,627 + Rru_A0467 0.64 -2.0 548,627 + Rru_A0467 0.64 -2.3 548,627 + Rru_A0467 0.64 -3.9 548,645 + Rru_A0467 0.65 -3.6 548,645 + Rru_A0467 0.65 -4.0 548,645 + Rru_A0467 0.65 -4.3 548,717 + Rru_A0467 0.69 -3.0 548,718 - Rru_A0467 0.69 -3.9 548,894 + Rru_A0467 0.79 -2.9 548,894 + Rru_A0467 0.79 -3.9 548,895 - Rru_A0467 0.79 -2.5 548,927 - Rru_A0467 0.81 -3.4 548,933 + Rru_A0467 0.82 -4.0 548,993 + Rru_A0467 0.85 -2.9 548,994 - Rru_A0467 0.85 -3.3 548,994 - Rru_A0467 0.85 -3.2 548,994 - Rru_A0467 0.85 -3.6 549,254 + -1.5 549,428 + -2.1 549,429 - -2.0 549,501 + Rru_A0468 0.22 -1.8 549,503 + Rru_A0468 0.23 -3.4 549,503 + Rru_A0468 0.23 -2.4 549,503 + Rru_A0468 0.23 -4.5 549,504 - Rru_A0468 0.23 -2.6 549,909 + +0.2 549,910 - +0.4 549,910 - -1.8 549,918 - -0.1 549,960 - -0.2 550,048 - -3.0 550,048 - -6.4 550,107 + -4.9 550,136 + -1.8 550,137 - -3.5
Or see this region's nucleotide sequence