Experiment: Gly-DL-Asp (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20575 and SM_b20576 are separated by 82 nucleotides SM_b20576 and SM_b20577 are separated by 10 nucleotides SM_b20577 and SM_b20578 are separated by 16 nucleotides
SM_b20575: SM_b20575 - 3-carboxy-cis,cis-muconate cycloisomerase, at 1,589,460 to 1,590,515
_b20575
SM_b20576: SM_b20576 - protocatechuate 3,4-dioxygenase subunit alpha, at 1,590,598 to 1,591,212
_b20576
SM_b20577: SM_b20577 - protocatechuate 3,4-dioxygenase subunit beta, at 1,591,223 to 1,591,984
_b20577
SM_b20578: SM_b20578 - gamma carboxymuconolactone decarboxylase, at 1,592,001 to 1,592,408
_b20578
Position (kb)
1590
1591
1592 Strain fitness (log2 ratio)
-1
0
1
2 at 1590.177 kb on - strand, within SM_b20575 at 1590.487 kb on + strand at 1590.653 kb on + strand at 1590.654 kb on - strand at 1590.654 kb on - strand at 1590.654 kb on - strand at 1590.657 kb on + strand at 1590.658 kb on - strand at 1590.758 kb on + strand, within SM_b20576 at 1590.758 kb on + strand, within SM_b20576 at 1590.759 kb on - strand, within SM_b20576 at 1590.759 kb on - strand, within SM_b20576 at 1590.759 kb on - strand, within SM_b20576 at 1590.759 kb on - strand, within SM_b20576 at 1590.780 kb on + strand, within SM_b20576 at 1590.965 kb on + strand, within SM_b20576 at 1590.965 kb on + strand, within SM_b20576 at 1591.109 kb on + strand, within SM_b20576 at 1591.112 kb on - strand, within SM_b20576 at 1591.157 kb on + strand at 1591.157 kb on + strand at 1591.158 kb on - strand at 1591.158 kb on - strand at 1591.212 kb on + strand at 1591.416 kb on + strand, within SM_b20577 at 1591.416 kb on + strand, within SM_b20577 at 1591.416 kb on + strand, within SM_b20577 at 1591.416 kb on + strand, within SM_b20577 at 1591.472 kb on - strand, within SM_b20577 at 1591.513 kb on - strand, within SM_b20577 at 1591.759 kb on + strand, within SM_b20577 at 1591.759 kb on + strand, within SM_b20577 at 1592.086 kb on + strand, within SM_b20578 at 1592.086 kb on + strand, within SM_b20578 at 1592.087 kb on - strand, within SM_b20578 at 1592.087 kb on - strand, within SM_b20578 at 1592.087 kb on - strand, within SM_b20578 at 1592.117 kb on + strand, within SM_b20578 at 1592.118 kb on - strand, within SM_b20578
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (N) remove 1,590,177 - SM_b20575 0.68 -1.2 1,590,487 + +1.6 1,590,653 + +0.1 1,590,654 - +1.7 1,590,654 - -1.1 1,590,654 - -0.3 1,590,657 + -0.3 1,590,658 - -0.9 1,590,758 + SM_b20576 0.26 -0.4 1,590,758 + SM_b20576 0.26 -0.4 1,590,759 - SM_b20576 0.26 -1.3 1,590,759 - SM_b20576 0.26 -1.4 1,590,759 - SM_b20576 0.26 -0.3 1,590,759 - SM_b20576 0.26 -0.1 1,590,780 + SM_b20576 0.30 -0.6 1,590,965 + SM_b20576 0.60 +1.7 1,590,965 + SM_b20576 0.60 +0.1 1,591,109 + SM_b20576 0.83 -0.1 1,591,112 - SM_b20576 0.84 +1.7 1,591,157 + -0.4 1,591,157 + +0.5 1,591,158 - +0.2 1,591,158 - +0.4 1,591,212 + -0.3 1,591,416 + SM_b20577 0.25 +0.1 1,591,416 + SM_b20577 0.25 -0.8 1,591,416 + SM_b20577 0.25 -0.2 1,591,416 + SM_b20577 0.25 +0.2 1,591,472 - SM_b20577 0.33 +0.1 1,591,513 - SM_b20577 0.38 -0.4 1,591,759 + SM_b20577 0.70 +0.6 1,591,759 + SM_b20577 0.70 +2.1 1,592,086 + SM_b20578 0.21 -1.2 1,592,086 + SM_b20578 0.21 -0.8 1,592,087 - SM_b20578 0.21 -0.3 1,592,087 - SM_b20578 0.21 -0.3 1,592,087 - SM_b20578 0.21 +2.7 1,592,117 + SM_b20578 0.28 -1.1 1,592,118 - SM_b20578 0.29 -0.7
Or see this region's nucleotide sequence