Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31685 and RR42_RS31690 are separated by 226 nucleotides RR42_RS31690 and RR42_RS31695 are separated by 63 nucleotides RR42_RS31695 and RR42_RS31700 are separated by 46 nucleotides RR42_RS31700 and RR42_RS31705 are separated by 8 nucleotides
RR42_RS31685: RR42_RS31685 - Asp/Glu/hydantoin racemase, at 2,488,467 to 2,489,087
_RS31685
RR42_RS31690: RR42_RS31690 - 4-oxalocrotonate tautomerase, at 2,489,314 to 2,489,727
_RS31690
RR42_RS31695: RR42_RS31695 - hypothetical protein, at 2,489,791 to 2,490,201
_RS31695
RR42_RS31700: RR42_RS31700 - esterase, at 2,490,248 to 2,491,141
_RS31700
RR42_RS31705: RR42_RS31705 - MBL fold metallo-hydrolase, at 2,491,150 to 2,492,133
_RS31705
Position (kb)
2489
2490
2491 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2488.839 kb on + strand, within RR42_RS31685 at 2488.839 kb on + strand, within RR42_RS31685 at 2488.839 kb on + strand, within RR42_RS31685 at 2488.951 kb on + strand, within RR42_RS31685 at 2488.952 kb on - strand, within RR42_RS31685 at 2488.979 kb on + strand, within RR42_RS31685 at 2489.069 kb on - strand at 2489.069 kb on - strand at 2489.069 kb on - strand at 2489.087 kb on + strand at 2489.088 kb on - strand at 2489.094 kb on - strand at 2489.283 kb on + strand at 2489.315 kb on + strand at 2489.315 kb on + strand at 2489.316 kb on - strand at 2489.399 kb on + strand, within RR42_RS31690 at 2489.400 kb on - strand, within RR42_RS31690 at 2489.400 kb on - strand, within RR42_RS31690 at 2489.415 kb on + strand, within RR42_RS31690 at 2489.518 kb on + strand, within RR42_RS31690 at 2489.519 kb on - strand, within RR42_RS31690 at 2489.697 kb on + strand at 2489.797 kb on - strand at 2489.797 kb on - strand at 2489.976 kb on - strand, within RR42_RS31695 at 2490.024 kb on - strand, within RR42_RS31695 at 2490.024 kb on - strand, within RR42_RS31695 at 2490.196 kb on - strand at 2490.223 kb on + strand at 2490.224 kb on - strand at 2490.426 kb on - strand, within RR42_RS31700 at 2490.525 kb on + strand, within RR42_RS31700 at 2490.525 kb on + strand, within RR42_RS31700 at 2490.612 kb on - strand, within RR42_RS31700 at 2490.635 kb on + strand, within RR42_RS31700 at 2490.636 kb on - strand, within RR42_RS31700 at 2490.780 kb on + strand, within RR42_RS31700 at 2490.841 kb on + strand, within RR42_RS31700 at 2490.841 kb on + strand, within RR42_RS31700 at 2490.972 kb on - strand, within RR42_RS31700 at 2490.972 kb on - strand, within RR42_RS31700 at 2490.972 kb on - strand, within RR42_RS31700 at 2490.972 kb on - strand, within RR42_RS31700 at 2491.001 kb on + strand, within RR42_RS31700 at 2491.134 kb on + strand at 2491.135 kb on - strand at 2491.156 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 2,488,839 + RR42_RS31685 0.60 +2.5 2,488,839 + RR42_RS31685 0.60 -1.2 2,488,839 + RR42_RS31685 0.60 +0.3 2,488,951 + RR42_RS31685 0.78 +0.4 2,488,952 - RR42_RS31685 0.78 +0.7 2,488,979 + RR42_RS31685 0.82 -0.6 2,489,069 - +0.1 2,489,069 - -0.3 2,489,069 - +0.8 2,489,087 + -0.9 2,489,088 - +0.2 2,489,094 - -0.2 2,489,283 + -1.1 2,489,315 + +0.2 2,489,315 + -0.2 2,489,316 - -0.7 2,489,399 + RR42_RS31690 0.21 -0.2 2,489,400 - RR42_RS31690 0.21 -0.2 2,489,400 - RR42_RS31690 0.21 -0.4 2,489,415 + RR42_RS31690 0.24 +0.2 2,489,518 + RR42_RS31690 0.49 -0.6 2,489,519 - RR42_RS31690 0.50 -0.7 2,489,697 + -0.3 2,489,797 - +0.9 2,489,797 - -0.2 2,489,976 - RR42_RS31695 0.45 +0.9 2,490,024 - RR42_RS31695 0.57 +0.2 2,490,024 - RR42_RS31695 0.57 +0.6 2,490,196 - -1.1 2,490,223 + -0.3 2,490,224 - -0.3 2,490,426 - RR42_RS31700 0.20 -0.6 2,490,525 + RR42_RS31700 0.31 +0.3 2,490,525 + RR42_RS31700 0.31 -0.6 2,490,612 - RR42_RS31700 0.41 -0.4 2,490,635 + RR42_RS31700 0.43 -0.3 2,490,636 - RR42_RS31700 0.43 -0.6 2,490,780 + RR42_RS31700 0.60 -1.6 2,490,841 + RR42_RS31700 0.66 +0.4 2,490,841 + RR42_RS31700 0.66 +0.7 2,490,972 - RR42_RS31700 0.81 -0.7 2,490,972 - RR42_RS31700 0.81 -1.1 2,490,972 - RR42_RS31700 0.81 -0.3 2,490,972 - RR42_RS31700 0.81 +0.7 2,491,001 + RR42_RS31700 0.84 +0.7 2,491,134 + -0.0 2,491,135 - -0.2 2,491,156 + -2.3
Or see this region's nucleotide sequence