Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14385 and RR42_RS14390 are separated by 40 nucleotides RR42_RS14390 and RR42_RS14395 are separated by 10 nucleotides RR42_RS14395 and RR42_RS14400 are separated by 16 nucleotides RR42_RS14400 and RR42_RS14405 are separated by 295 nucleotides RR42_RS14405 and RR42_RS14410 overlap by 1 nucleotides
RR42_RS14385: RR42_RS14385 - 3-isopropylmalate dehydrogenase, at 3,094,616 to 3,095,683
_RS14385
RR42_RS14390: RR42_RS14390 - 3-isopropylmalate dehydratase, at 3,095,724 to 3,096,374
_RS14390
RR42_RS14395: RR42_RS14395 - entericidin, at 3,096,385 to 3,096,516
_RS14395
RR42_RS14400: RR42_RS14400 - isopropylmalate isomerase, at 3,096,533 to 3,097,942
_RS14400
RR42_RS14405: RR42_RS14405 - leucine/isoleucine/valine transporter ATP-binding subunit, at 3,098,238 to 3,098,939
_RS14405
RR42_RS14410: RR42_RS14410 - leucine/isoleucine/valine transporter ATP-binding subunit, at 3,098,939 to 3,099,718
_RS14410
Position (kb)
3096
3097
3098 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 3095.779 kb on + strand at 3095.780 kb on - strand at 3095.780 kb on - strand at 3095.780 kb on - strand at 3095.780 kb on - strand at 3095.780 kb on - strand at 3095.845 kb on + strand, within RR42_RS14390 at 3095.846 kb on - strand, within RR42_RS14390 at 3096.013 kb on + strand, within RR42_RS14390 at 3096.070 kb on + strand, within RR42_RS14390 at 3096.358 kb on - strand at 3096.358 kb on - strand at 3096.368 kb on + strand at 3096.369 kb on - strand at 3096.386 kb on + strand at 3096.386 kb on + strand at 3096.387 kb on - strand at 3096.453 kb on + strand, within RR42_RS14395 at 3096.454 kb on - strand, within RR42_RS14395 at 3096.969 kb on + strand, within RR42_RS14400 at 3096.970 kb on - strand, within RR42_RS14400 at 3096.970 kb on - strand, within RR42_RS14400 at 3097.218 kb on + strand, within RR42_RS14400 at 3097.219 kb on - strand, within RR42_RS14400 at 3097.347 kb on + strand, within RR42_RS14400 at 3097.348 kb on - strand, within RR42_RS14400 at 3097.867 kb on - strand at 3097.927 kb on - strand at 3098.133 kb on + strand at 3098.134 kb on - strand at 3098.251 kb on + strand at 3098.252 kb on - strand at 3098.330 kb on - strand, within RR42_RS14405 at 3098.590 kb on + strand, within RR42_RS14405 at 3098.591 kb on - strand, within RR42_RS14405 at 3098.591 kb on - strand, within RR42_RS14405 at 3098.591 kb on - strand, within RR42_RS14405 at 3098.915 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 3,095,779 + -1.5 3,095,780 - -3.6 3,095,780 - -2.7 3,095,780 - -0.7 3,095,780 - -6.8 3,095,780 - -2.6 3,095,845 + RR42_RS14390 0.19 -2.0 3,095,846 - RR42_RS14390 0.19 -2.6 3,096,013 + RR42_RS14390 0.44 -3.9 3,096,070 + RR42_RS14390 0.53 -5.1 3,096,358 - -0.6 3,096,358 - -3.1 3,096,368 + -3.2 3,096,369 - -2.6 3,096,386 + -2.2 3,096,386 + -3.3 3,096,387 - -0.4 3,096,453 + RR42_RS14395 0.52 -2.0 3,096,454 - RR42_RS14395 0.52 -0.0 3,096,969 + RR42_RS14400 0.31 -2.9 3,096,970 - RR42_RS14400 0.31 -5.3 3,096,970 - RR42_RS14400 0.31 -1.2 3,097,218 + RR42_RS14400 0.49 -0.7 3,097,219 - RR42_RS14400 0.49 -5.1 3,097,347 + RR42_RS14400 0.58 -4.0 3,097,348 - RR42_RS14400 0.58 -2.0 3,097,867 - -4.0 3,097,927 - -3.4 3,098,133 + -0.7 3,098,134 - -0.2 3,098,251 + +0.1 3,098,252 - +0.2 3,098,330 - RR42_RS14405 0.13 +0.6 3,098,590 + RR42_RS14405 0.50 -0.2 3,098,591 - RR42_RS14405 0.50 +1.0 3,098,591 - RR42_RS14405 0.50 -1.2 3,098,591 - RR42_RS14405 0.50 -0.9 3,098,915 + +0.2
Or see this region's nucleotide sequence