Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12615 and RR42_RS12620 are separated by 382 nucleotides RR42_RS12620 and RR42_RS12625 are separated by 319 nucleotides RR42_RS12625 and RR42_RS12630 are separated by 224 nucleotides RR42_RS12630 and RR42_RS12635 are separated by 114 nucleotides
RR42_RS12615: RR42_RS12615 - LexA family transcriptional regulator, at 2,709,044 to 2,709,700
_RS12615
RR42_RS12620: RR42_RS12620 - tRNA-His, at 2,710,083 to 2,710,158
_RS12620
RR42_RS12625: RR42_RS12625 - hypothetical protein, at 2,710,478 to 2,711,002
_RS12625
RR42_RS12630: RR42_RS12630 - 5S ribosomal RNA, at 2,711,227 to 2,711,339
_RS12630
RR42_RS12635: RR42_RS12635 - 23S ribosomal RNA, at 2,711,454 to 2,714,355
_RS12635
Position (kb)
2710
2711
2712 Strain fitness (log2 ratio)
-1
0
1
2 at 2709.766 kb on - strand at 2709.778 kb on + strand at 2709.779 kb on - strand at 2709.804 kb on + strand at 2709.804 kb on + strand at 2709.805 kb on - strand at 2709.805 kb on - strand at 2709.887 kb on + strand at 2709.888 kb on - strand at 2709.931 kb on + strand at 2710.185 kb on + strand at 2710.229 kb on + strand at 2710.229 kb on + strand at 2710.230 kb on - strand at 2710.230 kb on - strand at 2710.240 kb on + strand at 2710.240 kb on + strand at 2710.257 kb on + strand at 2710.258 kb on - strand at 2710.375 kb on + strand at 2710.376 kb on - strand at 2710.441 kb on + strand at 2710.444 kb on + strand at 2710.445 kb on - strand at 2710.445 kb on - strand at 2710.445 kb on - strand at 2710.461 kb on - strand at 2710.486 kb on + strand at 2710.536 kb on + strand, within RR42_RS12625 at 2710.536 kb on + strand, within RR42_RS12625 at 2710.536 kb on + strand, within RR42_RS12625 at 2710.536 kb on + strand, within RR42_RS12625 at 2710.537 kb on - strand, within RR42_RS12625 at 2710.537 kb on - strand, within RR42_RS12625 at 2710.581 kb on - strand, within RR42_RS12625 at 2710.588 kb on + strand, within RR42_RS12625 at 2710.588 kb on + strand, within RR42_RS12625 at 2710.588 kb on + strand, within RR42_RS12625 at 2710.588 kb on + strand, within RR42_RS12625 at 2710.589 kb on - strand, within RR42_RS12625 at 2710.597 kb on - strand, within RR42_RS12625 at 2710.597 kb on - strand, within RR42_RS12625 at 2710.666 kb on - strand, within RR42_RS12625 at 2710.701 kb on + strand, within RR42_RS12625 at 2710.749 kb on - strand, within RR42_RS12625 at 2710.833 kb on - strand, within RR42_RS12625 at 2710.945 kb on - strand, within RR42_RS12625 at 2710.945 kb on - strand, within RR42_RS12625 at 2710.945 kb on - strand, within RR42_RS12625 at 2710.945 kb on - strand, within RR42_RS12625 at 2711.008 kb on + strand at 2711.008 kb on + strand at 2711.009 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 2,709,766 - -0.1 2,709,778 + -0.9 2,709,779 - +0.5 2,709,804 + -0.6 2,709,804 + -0.9 2,709,805 - -0.8 2,709,805 - -1.1 2,709,887 + +0.8 2,709,888 - -1.3 2,709,931 + -0.5 2,710,185 + +0.3 2,710,229 + -0.8 2,710,229 + +2.0 2,710,230 - -0.7 2,710,230 - +1.3 2,710,240 + +0.7 2,710,240 + -0.5 2,710,257 + -0.4 2,710,258 - +0.5 2,710,375 + +0.3 2,710,376 - -0.1 2,710,441 + +0.1 2,710,444 + +2.7 2,710,445 - -0.5 2,710,445 - +0.1 2,710,445 - -0.4 2,710,461 - -0.8 2,710,486 + +0.1 2,710,536 + RR42_RS12625 0.11 -0.5 2,710,536 + RR42_RS12625 0.11 -0.5 2,710,536 + RR42_RS12625 0.11 +0.0 2,710,536 + RR42_RS12625 0.11 +0.5 2,710,537 - RR42_RS12625 0.11 -1.0 2,710,537 - RR42_RS12625 0.11 +0.4 2,710,581 - RR42_RS12625 0.20 -1.1 2,710,588 + RR42_RS12625 0.21 -0.9 2,710,588 + RR42_RS12625 0.21 +0.3 2,710,588 + RR42_RS12625 0.21 -0.0 2,710,588 + RR42_RS12625 0.21 -0.9 2,710,589 - RR42_RS12625 0.21 -0.1 2,710,597 - RR42_RS12625 0.23 -0.5 2,710,597 - RR42_RS12625 0.23 -0.8 2,710,666 - RR42_RS12625 0.36 -0.6 2,710,701 + RR42_RS12625 0.42 -0.1 2,710,749 - RR42_RS12625 0.52 -0.4 2,710,833 - RR42_RS12625 0.68 +0.6 2,710,945 - RR42_RS12625 0.89 -0.4 2,710,945 - RR42_RS12625 0.89 -0.5 2,710,945 - RR42_RS12625 0.89 -0.6 2,710,945 - RR42_RS12625 0.89 +2.4 2,711,008 + -0.7 2,711,008 + -0.8 2,711,009 - +0.6
Or see this region's nucleotide sequence