Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01915 and RR42_RS01920 overlap by 4 nucleotides RR42_RS01920 and RR42_RS01925 are separated by 148 nucleotides RR42_RS01925 and RR42_RS01930 are separated by 99 nucleotides
RR42_RS01915: RR42_RS01915 - cytochrome C oxidase subunit I, at 429,622 to 430,725
_RS01915
RR42_RS01920: RR42_RS01920 - protoheme IX farnesyltransferase, at 430,722 to 431,657
_RS01920
RR42_RS01925: RR42_RS01925 - photosynthetic protein synthase I, at 431,806 to 432,432
_RS01925
RR42_RS01930: RR42_RS01930 - RNA polymerase factor sigma-32, at 432,532 to 433,461
_RS01930
Position (kb) 430
431
432 Strain fitness (log2 ratio) -2
-1
0
1
2 at 429.818 kb on - strand, within RR42_RS01915 at 429.929 kb on - strand, within RR42_RS01915 at 429.929 kb on - strand, within RR42_RS01915 at 430.273 kb on - strand, within RR42_RS01915 at 430.276 kb on + strand, within RR42_RS01915 at 430.276 kb on + strand, within RR42_RS01915 at 430.393 kb on + strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.655 kb on - strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.661 kb on + strand at 430.661 kb on + strand at 430.661 kb on + strand at 430.662 kb on - strand at 430.754 kb on + strand at 431.094 kb on + strand, within RR42_RS01920 at 431.155 kb on - strand, within RR42_RS01920 at 431.155 kb on - strand, within RR42_RS01920 at 431.426 kb on + strand, within RR42_RS01920 at 431.466 kb on - strand, within RR42_RS01920 at 431.481 kb on + strand, within RR42_RS01920 at 431.548 kb on - strand, within RR42_RS01920 at 431.592 kb on + strand at 431.592 kb on + strand at 431.592 kb on + strand at 431.631 kb on + strand at 431.631 kb on + strand at 431.632 kb on - strand at 431.632 kb on - strand at 431.738 kb on + strand at 431.739 kb on - strand at 431.739 kb on - strand at 431.739 kb on - strand at 432.046 kb on - strand, within RR42_RS01925 at 432.046 kb on - strand, within RR42_RS01925 at 432.046 kb on - strand, within RR42_RS01925 at 432.165 kb on + strand, within RR42_RS01925 at 432.165 kb on + strand, within RR42_RS01925 at 432.187 kb on + strand, within RR42_RS01925 at 432.187 kb on + strand, within RR42_RS01925 at 432.187 kb on + strand, within RR42_RS01925 at 432.187 kb on + strand, within RR42_RS01925 at 432.188 kb on - strand, within RR42_RS01925 at 432.277 kb on + strand, within RR42_RS01925 at 432.277 kb on + strand, within RR42_RS01925 at 432.277 kb on + strand, within RR42_RS01925 at 432.311 kb on - strand, within RR42_RS01925 at 432.311 kb on - strand, within RR42_RS01925 at 432.311 kb on - strand, within RR42_RS01925 at 432.337 kb on + strand, within RR42_RS01925 at 432.337 kb on + strand, within RR42_RS01925 at 432.338 kb on - strand, within RR42_RS01925 at 432.338 kb on - strand, within RR42_RS01925 at 432.338 kb on - strand, within RR42_RS01925 at 432.338 kb on - strand, within RR42_RS01925 at 432.477 kb on + strand at 432.511 kb on + strand at 432.512 kb on - strand at 432.526 kb on + strand at 432.526 kb on + strand at 432.526 kb on + strand at 432.527 kb on - strand at 432.527 kb on - strand at 432.527 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 429,818 - RR42_RS01915 0.18 -1.4 429,929 - RR42_RS01915 0.28 +0.6 429,929 - RR42_RS01915 0.28 -1.7 430,273 - RR42_RS01915 0.59 -0.3 430,276 + RR42_RS01915 0.59 -0.7 430,276 + RR42_RS01915 0.59 -1.5 430,393 + RR42_RS01915 0.70 -0.9 430,394 - RR42_RS01915 0.70 -0.1 430,394 - RR42_RS01915 0.70 -1.1 430,394 - RR42_RS01915 0.70 -0.3 430,655 - -0.1 430,659 + -0.6 430,659 + +0.8 430,659 + +0.4 430,659 + +0.0 430,659 + +0.8 430,661 + +0.1 430,661 + +0.0 430,661 + +1.0 430,662 - -1.2 430,754 + -2.2 431,094 + RR42_RS01920 0.40 -0.9 431,155 - RR42_RS01920 0.46 -1.7 431,155 - RR42_RS01920 0.46 -1.4 431,426 + RR42_RS01920 0.75 -1.9 431,466 - RR42_RS01920 0.79 -0.9 431,481 + RR42_RS01920 0.81 +0.5 431,548 - RR42_RS01920 0.88 +2.3 431,592 + -2.2 431,592 + +0.4 431,592 + -1.2 431,631 + -0.3 431,631 + -0.6 431,632 - -0.4 431,632 - -0.3 431,738 + -1.5 431,739 - +0.2 431,739 - +0.1 431,739 - +1.1 432,046 - RR42_RS01925 0.38 -0.7 432,046 - RR42_RS01925 0.38 -0.5 432,046 - RR42_RS01925 0.38 -0.0 432,165 + RR42_RS01925 0.57 +1.1 432,165 + RR42_RS01925 0.57 -0.9 432,187 + RR42_RS01925 0.61 +0.2 432,187 + RR42_RS01925 0.61 -0.2 432,187 + RR42_RS01925 0.61 +0.3 432,187 + RR42_RS01925 0.61 -0.4 432,188 - RR42_RS01925 0.61 -0.6 432,277 + RR42_RS01925 0.75 -0.1 432,277 + RR42_RS01925 0.75 +0.1 432,277 + RR42_RS01925 0.75 +0.0 432,311 - RR42_RS01925 0.81 +0.5 432,311 - RR42_RS01925 0.81 +0.3 432,311 - RR42_RS01925 0.81 -1.4 432,337 + RR42_RS01925 0.85 +0.3 432,337 + RR42_RS01925 0.85 -1.2 432,338 - RR42_RS01925 0.85 -1.2 432,338 - RR42_RS01925 0.85 -0.4 432,338 - RR42_RS01925 0.85 -0.6 432,338 - RR42_RS01925 0.85 +0.8 432,477 + +0.8 432,511 + -0.2 432,512 - -1.0 432,526 + -0.0 432,526 + -0.3 432,526 + +0.7 432,527 - +0.7 432,527 - +0.0 432,527 - -0.1
Or see this region's nucleotide sequence