Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_RNA25

Experiment: KB with Mitomycin C 0.625 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1550 and Psyr_1551 are separated by 28 nucleotidesPsyr_1551 and Psyr_RNA25 are separated by 92 nucleotidesPsyr_RNA25 and Psyr_1552 are separated by 58 nucleotides Psyr_1550: Psyr_1550 - Beta-lactamase-like protein, at 1,743,360 to 1,744,118 _1550 Psyr_1551: Psyr_1551 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 1,744,147 to 1,744,857 _1551 Psyr_RNA25: Psyr_RNA25 - tRNA-Ser, at 1,744,950 to 1,745,039 _RNA25 Psyr_1552: Psyr_1552 - CDS, at 1,745,098 to 1,745,496 _1552 Position (kb) 1744 1745 1746Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 1744.109 kb on + strandat 1744.125 kb on + strandat 1744.125 kb on + strandat 1744.125 kb on + strandat 1744.125 kb on + strandat 1744.125 kb on + strandat 1744.129 kb on + strandat 1744.129 kb on + strandat 1744.129 kb on + strandat 1744.129 kb on + strandat 1744.934 kb on + strandat 1744.996 kb on + strand, within Psyr_RNA25at 1745.012 kb on + strand, within Psyr_RNA25at 1745.040 kb on + strandat 1745.046 kb on + strandat 1745.047 kb on - strandat 1745.069 kb on + strandat 1745.080 kb on + strandat 1745.092 kb on + strandat 1745.113 kb on + strandat 1745.114 kb on - strandat 1745.187 kb on + strand, within Psyr_1552at 1745.187 kb on + strand, within Psyr_1552at 1745.187 kb on + strand, within Psyr_1552at 1745.187 kb on + strand, within Psyr_1552at 1745.187 kb on + strand, within Psyr_1552at 1745.187 kb on + strand, within Psyr_1552at 1745.188 kb on - strand, within Psyr_1552at 1745.188 kb on - strand, within Psyr_1552at 1745.188 kb on - strand, within Psyr_1552at 1745.188 kb on - strand, within Psyr_1552at 1745.188 kb on - strand, within Psyr_1552at 1745.262 kb on + strand, within Psyr_1552at 1745.262 kb on + strand, within Psyr_1552at 1745.263 kb on - strand, within Psyr_1552at 1745.263 kb on - strand, within Psyr_1552at 1745.263 kb on - strand, within Psyr_1552at 1745.263 kb on - strand, within Psyr_1552at 1745.263 kb on - strand, within Psyr_1552at 1745.269 kb on + strand, within Psyr_1552at 1745.269 kb on + strand, within Psyr_1552at 1745.269 kb on + strand, within Psyr_1552at 1745.269 kb on + strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.270 kb on - strand, within Psyr_1552at 1745.278 kb on - strand, within Psyr_1552at 1745.316 kb on + strand, within Psyr_1552at 1745.316 kb on + strand, within Psyr_1552at 1745.317 kb on - strand, within Psyr_1552at 1745.317 kb on - strand, within Psyr_1552at 1745.317 kb on - strand, within Psyr_1552at 1745.498 kb on + strandat 1745.498 kb on + strandat 1745.499 kb on - strandat 1745.499 kb on - strandat 1745.499 kb on - strandat 1745.551 kb on - strandat 1745.551 kb on - strandat 1745.551 kb on - strandat 1745.551 kb on - strandat 1745.584 kb on + strandat 1745.613 kb on + strandat 1745.613 kb on + strandat 1745.614 kb on - strandat 1745.655 kb on + strandat 1745.655 kb on + strandat 1745.700 kb on + strandat 1745.785 kb on - strandat 1745.875 kb on - strandat 1745.901 kb on + strandat 1745.950 kb on + strandat 1745.950 kb on + strandat 1745.950 kb on + strandat 1745.950 kb on + strandat 1745.950 kb on + strandat 1745.950 kb on + strandat 1745.950 kb on + strandat 1745.951 kb on - strandat 1745.951 kb on - strandat 1745.951 kb on - strandat 1745.951 kb on - strandat 1745.951 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml
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1,744,109 + +3.0
1,744,125 + -1.0
1,744,125 + +3.5
1,744,125 + -0.3
1,744,125 + +0.2
1,744,125 + +2.8
1,744,129 + +0.2
1,744,129 + +3.6
1,744,129 + +0.2
1,744,129 + +0.2
1,744,934 + +3.5
1,744,996 + Psyr_RNA25 0.51 +0.8
1,745,012 + Psyr_RNA25 0.69 +0.2
1,745,040 + -0.1
1,745,046 + +3.4
1,745,047 - -3.9
1,745,069 + -1.7
1,745,080 + +1.2
1,745,092 + -0.2
1,745,113 + +1.2
1,745,114 - -1.2
1,745,187 + Psyr_1552 0.22 +0.2
1,745,187 + Psyr_1552 0.22 +0.2
1,745,187 + Psyr_1552 0.22 +0.2
1,745,187 + Psyr_1552 0.22 -1.6
1,745,187 + Psyr_1552 0.22 +0.2
1,745,187 + Psyr_1552 0.22 +0.9
1,745,188 - Psyr_1552 0.23 +0.2
1,745,188 - Psyr_1552 0.23 +0.2
1,745,188 - Psyr_1552 0.23 +0.2
1,745,188 - Psyr_1552 0.23 -4.1
1,745,188 - Psyr_1552 0.23 -1.7
1,745,262 + Psyr_1552 0.41 -1.4
1,745,262 + Psyr_1552 0.41 +2.8
1,745,263 - Psyr_1552 0.41 -2.4
1,745,263 - Psyr_1552 0.41 +0.6
1,745,263 - Psyr_1552 0.41 -2.5
1,745,263 - Psyr_1552 0.41 -5.7
1,745,263 - Psyr_1552 0.41 +3.4
1,745,269 + Psyr_1552 0.43 +3.5
1,745,269 + Psyr_1552 0.43 +2.2
1,745,269 + Psyr_1552 0.43 -0.5
1,745,269 + Psyr_1552 0.43 +0.0
1,745,270 - Psyr_1552 0.43 -0.8
1,745,270 - Psyr_1552 0.43 +2.5
1,745,270 - Psyr_1552 0.43 +0.2
1,745,270 - Psyr_1552 0.43 -0.5
1,745,270 - Psyr_1552 0.43 +0.2
1,745,270 - Psyr_1552 0.43 +0.2
1,745,270 - Psyr_1552 0.43 +3.0
1,745,270 - Psyr_1552 0.43 +1.6
1,745,270 - Psyr_1552 0.43 +3.0
1,745,270 - Psyr_1552 0.43 -0.2
1,745,270 - Psyr_1552 0.43 -4.3
1,745,270 - Psyr_1552 0.43 +3.8
1,745,270 - Psyr_1552 0.43 -0.5
1,745,270 - Psyr_1552 0.43 +4.1
1,745,278 - Psyr_1552 0.45 +3.5
1,745,316 + Psyr_1552 0.55 +1.2
1,745,316 + Psyr_1552 0.55 -0.9
1,745,317 - Psyr_1552 0.55 +2.5
1,745,317 - Psyr_1552 0.55 -0.8
1,745,317 - Psyr_1552 0.55 -3.5
1,745,498 + -4.6
1,745,498 + +1.8
1,745,499 - -2.9
1,745,499 - +1.8
1,745,499 - +0.2
1,745,551 - +3.8
1,745,551 - +0.2
1,745,551 - +2.2
1,745,551 - +3.6
1,745,584 + +1.8
1,745,613 + +0.2
1,745,613 + +0.2
1,745,614 - -0.2
1,745,655 + +0.2
1,745,655 + +1.2
1,745,700 + +0.2
1,745,785 - -1.2
1,745,875 - -4.6
1,745,901 + -0.6
1,745,950 + -2.4
1,745,950 + +1.2
1,745,950 + -1.4
1,745,950 + +2.5
1,745,950 + -4.1
1,745,950 + -2.3
1,745,950 + -0.7
1,745,951 - +0.1
1,745,951 - +2.2
1,745,951 - -1.4
1,745,951 - -2.5
1,745,951 - +1.4

Or see this region's nucleotide sequence