Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4354 and Psyr_4355 are separated by 5 nucleotides Psyr_4355 and Psyr_4356 are separated by 147 nucleotides Psyr_4356 and Psyr_4357 are separated by 83 nucleotides Psyr_4357 and Psyr_4358 are separated by 291 nucleotides
Psyr_4354: Psyr_4354 - DNA polymerase III, delta subunit, at 5,208,672 to 5,209,709
_4354
Psyr_4355: Psyr_4355 - hypothetical protein, at 5,209,715 to 5,209,927
_4355
Psyr_4356: Psyr_4356 - conserved hypothetical protein, at 5,210,075 to 5,210,230
_4356
Psyr_4357: Psyr_4357 - HopAJ2 protein, at 5,210,314 to 5,211,651
_4357
Psyr_4358: Psyr_4358 - Lipoate synthase, at 5,211,943 to 5,212,950
_4358
Position (kb)
5210
5211
5212 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 5209.708 kb on - strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.729 kb on + strand at 5209.729 kb on + strand at 5209.729 kb on + strand at 5209.729 kb on + strand at 5209.730 kb on - strand at 5209.730 kb on - strand at 5209.730 kb on - strand at 5209.730 kb on - strand at 5209.811 kb on + strand, within Psyr_4355 at 5209.811 kb on + strand, within Psyr_4355 at 5209.811 kb on + strand, within Psyr_4355 at 5209.812 kb on - strand, within Psyr_4355 at 5209.812 kb on - strand, within Psyr_4355 at 5209.812 kb on - strand, within Psyr_4355 at 5209.818 kb on + strand, within Psyr_4355 at 5209.819 kb on - strand, within Psyr_4355 at 5209.953 kb on + strand at 5209.954 kb on - strand at 5209.954 kb on - strand at 5209.954 kb on - strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.980 kb on - strand at 5209.980 kb on - strand at 5209.980 kb on - strand at 5209.983 kb on + strand at 5209.983 kb on + strand at 5209.984 kb on - strand at 5209.984 kb on - strand at 5209.984 kb on - strand at 5209.995 kb on + strand at 5209.995 kb on + strand at 5209.996 kb on - strand at 5209.996 kb on - strand at 5210.006 kb on + strand at 5210.049 kb on + strand at 5210.049 kb on + strand at 5210.049 kb on + strand at 5210.050 kb on - strand at 5210.050 kb on - strand at 5210.050 kb on - strand at 5210.050 kb on - strand at 5210.194 kb on + strand, within Psyr_4356 at 5210.194 kb on + strand, within Psyr_4356 at 5210.195 kb on - strand, within Psyr_4356 at 5210.218 kb on + strand at 5210.219 kb on - strand at 5210.289 kb on - strand at 5210.297 kb on + strand at 5210.297 kb on + strand at 5210.582 kb on + strand, within Psyr_4357 at 5210.582 kb on + strand, within Psyr_4357 at 5210.583 kb on - strand, within Psyr_4357 at 5210.583 kb on - strand, within Psyr_4357 at 5210.675 kb on - strand, within Psyr_4357 at 5210.675 kb on - strand, within Psyr_4357 at 5210.734 kb on + strand, within Psyr_4357 at 5210.767 kb on + strand, within Psyr_4357 at 5210.815 kb on - strand, within Psyr_4357 at 5210.872 kb on + strand, within Psyr_4357 at 5210.872 kb on + strand, within Psyr_4357 at 5210.873 kb on - strand, within Psyr_4357 at 5210.945 kb on + strand, within Psyr_4357 at 5210.946 kb on - strand, within Psyr_4357 at 5210.946 kb on - strand, within Psyr_4357 at 5210.946 kb on - strand, within Psyr_4357 at 5210.946 kb on - strand, within Psyr_4357 at 5211.013 kb on + strand, within Psyr_4357 at 5211.013 kb on + strand, within Psyr_4357 at 5211.013 kb on + strand, within Psyr_4357 at 5211.013 kb on + strand, within Psyr_4357 at 5211.013 kb on + strand, within Psyr_4357 at 5211.013 kb on + strand, within Psyr_4357 at 5211.013 kb on + strand, within Psyr_4357 at 5211.013 kb on + strand, within Psyr_4357 at 5211.013 kb on + strand, within Psyr_4357 at 5211.013 kb on + strand, within Psyr_4357 at 5211.014 kb on - strand, within Psyr_4357 at 5211.014 kb on - strand, within Psyr_4357 at 5211.014 kb on - strand, within Psyr_4357 at 5211.014 kb on - strand, within Psyr_4357 at 5211.014 kb on - strand, within Psyr_4357 at 5211.014 kb on - strand, within Psyr_4357 at 5211.014 kb on - strand, within Psyr_4357 at 5211.014 kb on - strand, within Psyr_4357 at 5211.014 kb on - strand, within Psyr_4357 at 5211.014 kb on - strand, within Psyr_4357 at 5211.058 kb on - strand, within Psyr_4357 at 5211.058 kb on - strand, within Psyr_4357 at 5211.100 kb on + strand, within Psyr_4357 at 5211.100 kb on + strand, within Psyr_4357 at 5211.101 kb on - strand, within Psyr_4357 at 5211.356 kb on - strand, within Psyr_4357 at 5211.356 kb on - strand, within Psyr_4357 at 5211.356 kb on - strand, within Psyr_4357 at 5211.356 kb on - strand, within Psyr_4357 at 5211.421 kb on + strand, within Psyr_4357 at 5211.421 kb on + strand, within Psyr_4357 at 5211.422 kb on - strand, within Psyr_4357 at 5211.422 kb on - strand, within Psyr_4357 at 5211.509 kb on - strand, within Psyr_4357 at 5211.546 kb on + strand at 5211.547 kb on - strand at 5211.715 kb on + strand at 5211.716 kb on - strand at 5211.787 kb on + strand at 5211.787 kb on + strand at 5211.787 kb on + strand at 5211.787 kb on + strand at 5211.854 kb on + strand at 5211.854 kb on + strand at 5211.854 kb on + strand at 5211.854 kb on + strand at 5211.855 kb on - strand at 5211.912 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 5,209,708 - +0.2 5,209,725 + +0.1 5,209,725 + +1.1 5,209,725 + +2.1 5,209,725 + +0.2 5,209,725 + -1.0 5,209,725 + -4.0 5,209,725 + -2.3 5,209,726 - -0.1 5,209,726 - -1.3 5,209,726 - +2.1 5,209,726 - -1.1 5,209,726 - -0.5 5,209,726 - -2.6 5,209,726 - +1.3 5,209,726 - +0.6 5,209,726 - -0.8 5,209,729 + +2.4 5,209,729 + +0.1 5,209,729 + +1.1 5,209,729 + +2.4 5,209,730 - -1.4 5,209,730 - +1.4 5,209,730 - -2.5 5,209,730 - +3.1 5,209,811 + Psyr_4355 0.45 +2.6 5,209,811 + Psyr_4355 0.45 +2.9 5,209,811 + Psyr_4355 0.45 +0.7 5,209,812 - Psyr_4355 0.46 +1.6 5,209,812 - Psyr_4355 0.46 +4.4 5,209,812 - Psyr_4355 0.46 +0.1 5,209,818 + Psyr_4355 0.48 +0.1 5,209,819 - Psyr_4355 0.49 +4.1 5,209,953 + +0.1 5,209,954 - +0.1 5,209,954 - -0.5 5,209,954 - +1.1 5,209,979 + +2.6 5,209,979 + +0.1 5,209,979 + -2.1 5,209,979 + +1.1 5,209,979 + +2.6 5,209,979 + -1.1 5,209,979 + -3.4 5,209,980 - +3.1 5,209,980 - -2.0 5,209,980 - -1.0 5,209,983 + -3.0 5,209,983 + +3.5 5,209,984 - -0.2 5,209,984 - +1.6 5,209,984 - -2.8 5,209,995 + -2.9 5,209,995 + -2.2 5,209,996 - -3.5 5,209,996 - +0.2 5,210,006 + -0.8 5,210,049 + -3.1 5,210,049 + +0.1 5,210,049 + +1.6 5,210,050 - +1.2 5,210,050 - -4.3 5,210,050 - -2.1 5,210,050 - +4.2 5,210,194 + Psyr_4356 0.76 -3.3 5,210,194 + Psyr_4356 0.76 -4.9 5,210,195 - Psyr_4356 0.77 +1.1 5,210,218 + +0.1 5,210,219 - -3.3 5,210,289 - -3.0 5,210,297 + -1.5 5,210,297 + -2.4 5,210,582 + Psyr_4357 0.20 +0.1 5,210,582 + Psyr_4357 0.20 -2.5 5,210,583 - Psyr_4357 0.20 -3.3 5,210,583 - Psyr_4357 0.20 -2.3 5,210,675 - Psyr_4357 0.27 +1.6 5,210,675 - Psyr_4357 0.27 -1.6 5,210,734 + Psyr_4357 0.31 -2.1 5,210,767 + Psyr_4357 0.34 +2.4 5,210,815 - Psyr_4357 0.37 -0.8 5,210,872 + Psyr_4357 0.42 +0.1 5,210,872 + Psyr_4357 0.42 -3.0 5,210,873 - Psyr_4357 0.42 -2.2 5,210,945 + Psyr_4357 0.47 -1.8 5,210,946 - Psyr_4357 0.47 -1.4 5,210,946 - Psyr_4357 0.47 +2.1 5,210,946 - Psyr_4357 0.47 -0.5 5,210,946 - Psyr_4357 0.47 -4.0 5,211,013 + Psyr_4357 0.52 -4.2 5,211,013 + Psyr_4357 0.52 -5.8 5,211,013 + Psyr_4357 0.52 -3.6 5,211,013 + Psyr_4357 0.52 +1.1 5,211,013 + Psyr_4357 0.52 +1.6 5,211,013 + Psyr_4357 0.52 -2.5 5,211,013 + Psyr_4357 0.52 -2.4 5,211,013 + Psyr_4357 0.52 -2.4 5,211,013 + Psyr_4357 0.52 -3.6 5,211,013 + Psyr_4357 0.52 -1.9 5,211,014 - Psyr_4357 0.52 -3.9 5,211,014 - Psyr_4357 0.52 -1.2 5,211,014 - Psyr_4357 0.52 -2.9 5,211,014 - Psyr_4357 0.52 -1.5 5,211,014 - Psyr_4357 0.52 +0.9 5,211,014 - Psyr_4357 0.52 -0.5 5,211,014 - Psyr_4357 0.52 -2.2 5,211,014 - Psyr_4357 0.52 +2.4 5,211,014 - Psyr_4357 0.52 +2.9 5,211,014 - Psyr_4357 0.52 -4.5 5,211,058 - Psyr_4357 0.56 -3.1 5,211,058 - Psyr_4357 0.56 +1.1 5,211,100 + Psyr_4357 0.59 +1.1 5,211,100 + Psyr_4357 0.59 -2.2 5,211,101 - Psyr_4357 0.59 +2.1 5,211,356 - Psyr_4357 0.78 +2.4 5,211,356 - Psyr_4357 0.78 -0.6 5,211,356 - Psyr_4357 0.78 +2.9 5,211,356 - Psyr_4357 0.78 -3.6 5,211,421 + Psyr_4357 0.83 -2.3 5,211,421 + Psyr_4357 0.83 -0.9 5,211,422 - Psyr_4357 0.83 -4.2 5,211,422 - Psyr_4357 0.83 -2.2 5,211,509 - Psyr_4357 0.89 -5.0 5,211,546 + -1.6 5,211,547 - +0.1 5,211,715 + -0.9 5,211,716 - +0.1 5,211,787 + +4.1 5,211,787 + -1.2 5,211,787 + +1.8 5,211,787 + +3.4 5,211,854 + +4.7 5,211,854 + -0.7 5,211,854 + -0.6 5,211,854 + +1.1 5,211,855 - -2.4 5,211,912 - -4.3
Or see this region's nucleotide sequence