Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4188 and Psyr_4189 are separated by 103 nucleotides Psyr_4189 and Psyr_4190 are separated by 79 nucleotides Psyr_4190 and Psyr_4191 overlap by 4 nucleotides Psyr_4191 and Psyr_4192 are separated by 136 nucleotides
Psyr_4188: Psyr_4188 - 23S rRNA Um-2552 2'-O-methyltransferase, at 4,982,078 to 4,982,728
_4188
Psyr_4189: Psyr_4189 - Protein of unknown function UPF0044, at 4,982,832 to 4,983,143
_4189
Psyr_4190: Psyr_4190 - transcription elongation factor GreA, at 4,983,223 to 4,983,699
_4190
Psyr_4191: Psyr_4191 - carbamoyl-phosphate synthase large subunit, at 4,983,696 to 4,986,917
_4191
Psyr_4192: Psyr_4192 - carbamoyl-phosphate synthase small subunit, at 4,987,054 to 4,988,190
_4192
Position (kb) 4983
4984
4985
4986
4987 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 4982.760 kb on - strand at 4982.832 kb on - strand at 4982.832 kb on - strand at 4982.863 kb on - strand at 4982.863 kb on - strand at 4982.863 kb on - strand at 4982.863 kb on - strand at 4982.863 kb on - strand at 4982.930 kb on - strand, within Psyr_4189 at 4983.104 kb on - strand, within Psyr_4189 at 4983.104 kb on - strand, within Psyr_4189 at 4983.206 kb on - strand at 4983.229 kb on - strand at 4983.229 kb on - strand at 4983.229 kb on - strand at 4983.387 kb on - strand, within Psyr_4190 at 4983.557 kb on + strand, within Psyr_4190 at 4983.557 kb on + strand, within Psyr_4190 at 4983.690 kb on - strand at 4987.032 kb on - strand at 4987.032 kb on - strand at 4987.032 kb on - strand at 4987.032 kb on - strand at 4987.035 kb on - strand at 4987.035 kb on - strand at 4987.035 kb on - strand at 4987.035 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 4,982,760 - +0.4 4,982,832 - +3.1 4,982,832 - -2.0 4,982,863 - +2.9 4,982,863 - +2.4 4,982,863 - -1.5 4,982,863 - +2.9 4,982,863 - -1.4 4,982,930 - Psyr_4189 0.31 -3.9 4,983,104 - Psyr_4189 0.87 +0.1 4,983,104 - Psyr_4189 0.87 -0.7 4,983,206 - -0.0 4,983,229 - +4.2 4,983,229 - +2.4 4,983,229 - -2.2 4,983,387 - Psyr_4190 0.34 +0.1 4,983,557 + Psyr_4190 0.70 -2.1 4,983,557 + Psyr_4190 0.70 +0.1 4,983,690 - -2.6 4,987,032 - +1.0 4,987,032 - +4.2 4,987,032 - +3.1 4,987,032 - -0.0 4,987,035 - +3.4 4,987,035 - +3.7 4,987,035 - +3.4 4,987,035 - +2.3
Or see this region's nucleotide sequence