Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3671 and Psyr_3672 are separated by 98 nucleotides Psyr_3672 and Psyr_3673 are separated by 1 nucleotides Psyr_3673 and Psyr_3674 overlap by 4 nucleotides
Psyr_3671: Psyr_3671 - Major facilitator superfamily, at 4,357,799 to 4,359,007
_3671
Psyr_3672: Psyr_3672 - cobalamin-5'-phosphate synthase, at 4,359,106 to 4,359,837
_3672
Psyr_3673: Psyr_3673 - Phosphoglycerate/bisphosphoglycerate mutase, at 4,359,839 to 4,360,411
_3673
Psyr_3674: Psyr_3674 - Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase, at 4,360,408 to 4,361,460
_3674
Position (kb) 4359
4360
4361 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 4359.032 kb on + strand at 4359.032 kb on + strand at 4359.032 kb on + strand at 4359.032 kb on + strand at 4359.032 kb on + strand at 4359.032 kb on + strand at 4359.032 kb on + strand at 4359.033 kb on - strand at 4359.033 kb on - strand at 4359.033 kb on - strand at 4359.033 kb on - strand at 4359.169 kb on - strand at 4359.368 kb on + strand, within Psyr_3672 at 4359.368 kb on + strand, within Psyr_3672 at 4359.368 kb on + strand, within Psyr_3672 at 4359.368 kb on + strand, within Psyr_3672 at 4359.368 kb on + strand, within Psyr_3672 at 4359.369 kb on - strand, within Psyr_3672 at 4359.647 kb on - strand, within Psyr_3672 at 4359.737 kb on + strand, within Psyr_3672 at 4360.002 kb on + strand, within Psyr_3673 at 4360.002 kb on + strand, within Psyr_3673 at 4360.087 kb on - strand, within Psyr_3673 at 4360.087 kb on - strand, within Psyr_3673 at 4360.490 kb on - strand at 4360.640 kb on - strand, within Psyr_3674 at 4360.715 kb on + strand, within Psyr_3674 at 4360.988 kb on - strand, within Psyr_3674 at 4360.988 kb on - strand, within Psyr_3674 at 4361.229 kb on - strand, within Psyr_3674
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 4,359,032 + -3.1 4,359,032 + +1.1 4,359,032 + -1.9 4,359,032 + -1.3 4,359,032 + +0.1 4,359,032 + -1.7 4,359,032 + +3.8 4,359,033 - +0.1 4,359,033 - +4.6 4,359,033 - +1.1 4,359,033 - +2.4 4,359,169 - -1.1 4,359,368 + Psyr_3672 0.36 -3.1 4,359,368 + Psyr_3672 0.36 +2.1 4,359,368 + Psyr_3672 0.36 +2.9 4,359,368 + Psyr_3672 0.36 -4.0 4,359,368 + Psyr_3672 0.36 +2.7 4,359,369 - Psyr_3672 0.36 +1.7 4,359,647 - Psyr_3672 0.74 +3.5 4,359,737 + Psyr_3672 0.86 +0.1 4,360,002 + Psyr_3673 0.28 -2.1 4,360,002 + Psyr_3673 0.28 +1.7 4,360,087 - Psyr_3673 0.43 -4.2 4,360,087 - Psyr_3673 0.43 +0.1 4,360,490 - -0.0 4,360,640 - Psyr_3674 0.22 +3.9 4,360,715 + Psyr_3674 0.29 +3.4 4,360,988 - Psyr_3674 0.55 -1.0 4,360,988 - Psyr_3674 0.55 +2.9 4,361,229 - Psyr_3674 0.78 +0.1
Or see this region's nucleotide sequence