Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3579 and Psyr_3580 are separated by 31 nucleotides Psyr_3580 and Psyr_3581 are separated by 271 nucleotides
Psyr_3579: Psyr_3579 - Class I peptide chain release factor, at 4,262,499 to 4,262,912
_3579
Psyr_3580: Psyr_3580 - aromatic amino acid:proton symporter, AAT family, at 4,262,944 to 4,264,353
_3580
Psyr_3581: Psyr_3581 - ribosomal large subunit pseudouridine synthase B, at 4,264,625 to 4,265,854
_3581
Position (kb)
4262
4263
4264
4265 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 4262.305 kb on + strand at 4262.306 kb on - strand at 4262.338 kb on - strand at 4262.338 kb on - strand at 4262.448 kb on + strand at 4262.448 kb on + strand at 4262.449 kb on - strand at 4262.458 kb on + strand at 4262.458 kb on + strand at 4262.458 kb on + strand at 4262.459 kb on - strand at 4262.460 kb on + strand at 4262.758 kb on + strand, within Psyr_3579 at 4262.759 kb on - strand, within Psyr_3579 at 4262.945 kb on + strand at 4262.945 kb on + strand at 4262.945 kb on + strand at 4262.945 kb on + strand at 4262.946 kb on - strand at 4262.946 kb on - strand at 4262.946 kb on - strand at 4262.946 kb on - strand at 4262.946 kb on - strand at 4262.946 kb on - strand at 4262.946 kb on - strand at 4262.946 kb on - strand at 4262.946 kb on - strand at 4262.976 kb on + strand at 4262.977 kb on - strand at 4263.035 kb on + strand at 4263.036 kb on - strand at 4263.107 kb on + strand, within Psyr_3580 at 4263.107 kb on + strand, within Psyr_3580 at 4263.107 kb on + strand, within Psyr_3580 at 4263.107 kb on + strand, within Psyr_3580 at 4263.107 kb on + strand, within Psyr_3580 at 4263.107 kb on + strand, within Psyr_3580 at 4263.107 kb on + strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.108 kb on - strand, within Psyr_3580 at 4263.272 kb on + strand, within Psyr_3580 at 4263.272 kb on + strand, within Psyr_3580 at 4263.273 kb on - strand, within Psyr_3580 at 4263.413 kb on + strand, within Psyr_3580 at 4263.413 kb on + strand, within Psyr_3580 at 4263.413 kb on + strand, within Psyr_3580 at 4263.413 kb on + strand, within Psyr_3580 at 4263.413 kb on + strand, within Psyr_3580 at 4263.414 kb on - strand, within Psyr_3580 at 4263.414 kb on - strand, within Psyr_3580 at 4263.414 kb on - strand, within Psyr_3580 at 4263.414 kb on - strand, within Psyr_3580 at 4263.428 kb on + strand, within Psyr_3580 at 4263.429 kb on - strand, within Psyr_3580 at 4263.528 kb on - strand, within Psyr_3580 at 4263.609 kb on - strand, within Psyr_3580 at 4263.609 kb on - strand, within Psyr_3580 at 4263.626 kb on + strand, within Psyr_3580 at 4263.626 kb on + strand, within Psyr_3580 at 4263.627 kb on - strand, within Psyr_3580 at 4263.656 kb on - strand, within Psyr_3580 at 4263.656 kb on - strand, within Psyr_3580 at 4263.836 kb on + strand, within Psyr_3580 at 4263.836 kb on + strand, within Psyr_3580 at 4263.836 kb on + strand, within Psyr_3580 at 4263.836 kb on + strand, within Psyr_3580 at 4263.836 kb on + strand, within Psyr_3580 at 4263.837 kb on - strand, within Psyr_3580 at 4263.837 kb on - strand, within Psyr_3580 at 4263.837 kb on - strand, within Psyr_3580 at 4263.837 kb on - strand, within Psyr_3580 at 4263.837 kb on - strand, within Psyr_3580 at 4263.837 kb on - strand, within Psyr_3580 at 4263.837 kb on - strand, within Psyr_3580 at 4263.837 kb on - strand, within Psyr_3580 at 4263.837 kb on - strand, within Psyr_3580 at 4264.004 kb on + strand, within Psyr_3580 at 4264.004 kb on + strand, within Psyr_3580 at 4264.004 kb on + strand, within Psyr_3580 at 4264.004 kb on + strand, within Psyr_3580 at 4264.004 kb on + strand, within Psyr_3580 at 4264.004 kb on + strand, within Psyr_3580 at 4264.004 kb on + strand, within Psyr_3580 at 4264.005 kb on - strand, within Psyr_3580 at 4264.005 kb on - strand, within Psyr_3580 at 4264.005 kb on - strand, within Psyr_3580 at 4264.005 kb on - strand, within Psyr_3580 at 4264.005 kb on - strand, within Psyr_3580 at 4264.046 kb on + strand, within Psyr_3580 at 4264.046 kb on + strand, within Psyr_3580 at 4264.046 kb on + strand, within Psyr_3580 at 4264.047 kb on - strand, within Psyr_3580 at 4264.047 kb on - strand, within Psyr_3580 at 4264.047 kb on - strand, within Psyr_3580 at 4264.047 kb on - strand, within Psyr_3580 at 4264.047 kb on - strand, within Psyr_3580 at 4264.047 kb on - strand, within Psyr_3580 at 4264.047 kb on - strand, within Psyr_3580 at 4264.047 kb on - strand, within Psyr_3580 at 4264.047 kb on - strand, within Psyr_3580 at 4264.097 kb on + strand, within Psyr_3580 at 4264.098 kb on - strand, within Psyr_3580 at 4264.098 kb on - strand, within Psyr_3580 at 4264.098 kb on - strand, within Psyr_3580 at 4264.391 kb on + strand at 4264.391 kb on + strand at 4264.391 kb on + strand at 4264.392 kb on - strand at 4264.392 kb on - strand at 4264.491 kb on + strand at 4264.491 kb on + strand at 4264.491 kb on + strand at 4264.491 kb on + strand at 4264.492 kb on - strand at 4265.228 kb on + strand, within Psyr_3581 at 4265.228 kb on + strand, within Psyr_3581 at 4265.228 kb on + strand, within Psyr_3581 at 4265.229 kb on - strand, within Psyr_3581 at 4265.229 kb on - strand, within Psyr_3581
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 4,262,305 + +0.1 4,262,306 - +2.3 4,262,338 - -4.2 4,262,338 - +0.1 4,262,448 + +2.4 4,262,448 + -0.7 4,262,449 - +2.4 4,262,458 + -2.3 4,262,458 + +0.9 4,262,458 + +2.1 4,262,459 - +3.8 4,262,460 + +4.3 4,262,758 + Psyr_3579 0.63 +1.4 4,262,759 - Psyr_3579 0.63 +2.9 4,262,945 + +2.6 4,262,945 + -2.9 4,262,945 + -1.5 4,262,945 + +0.1 4,262,946 - +0.3 4,262,946 - +3.2 4,262,946 - -2.8 4,262,946 - +2.1 4,262,946 - +2.6 4,262,946 - -1.9 4,262,946 - -1.3 4,262,946 - -2.4 4,262,946 - -0.9 4,262,976 + -2.0 4,262,977 - +2.1 4,263,035 + -2.1 4,263,036 - -0.8 4,263,107 + Psyr_3580 0.12 -4.2 4,263,107 + Psyr_3580 0.12 +2.1 4,263,107 + Psyr_3580 0.12 -0.7 4,263,107 + Psyr_3580 0.12 +2.4 4,263,107 + Psyr_3580 0.12 -1.6 4,263,107 + Psyr_3580 0.12 +0.1 4,263,107 + Psyr_3580 0.12 -0.2 4,263,108 - Psyr_3580 0.12 +1.8 4,263,108 - Psyr_3580 0.12 +2.6 4,263,108 - Psyr_3580 0.12 +0.5 4,263,108 - Psyr_3580 0.12 +4.3 4,263,108 - Psyr_3580 0.12 +3.5 4,263,108 - Psyr_3580 0.12 +1.1 4,263,108 - Psyr_3580 0.12 -2.0 4,263,108 - Psyr_3580 0.12 -2.6 4,263,108 - Psyr_3580 0.12 -2.6 4,263,108 - Psyr_3580 0.12 -4.4 4,263,108 - Psyr_3580 0.12 -3.5 4,263,108 - Psyr_3580 0.12 -2.5 4,263,108 - Psyr_3580 0.12 -0.9 4,263,272 + Psyr_3580 0.23 +2.6 4,263,272 + Psyr_3580 0.23 -0.4 4,263,273 - Psyr_3580 0.23 +0.1 4,263,413 + Psyr_3580 0.33 +0.1 4,263,413 + Psyr_3580 0.33 +0.1 4,263,413 + Psyr_3580 0.33 +2.9 4,263,413 + Psyr_3580 0.33 -0.6 4,263,413 + Psyr_3580 0.33 +1.6 4,263,414 - Psyr_3580 0.33 -5.0 4,263,414 - Psyr_3580 0.33 +2.9 4,263,414 - Psyr_3580 0.33 -4.5 4,263,414 - Psyr_3580 0.33 +2.6 4,263,428 + Psyr_3580 0.34 +2.6 4,263,429 - Psyr_3580 0.34 -1.5 4,263,528 - Psyr_3580 0.41 +1.2 4,263,609 - Psyr_3580 0.47 -2.2 4,263,609 - Psyr_3580 0.47 +1.1 4,263,626 + Psyr_3580 0.48 -0.3 4,263,626 + Psyr_3580 0.48 -1.1 4,263,627 - Psyr_3580 0.48 -2.9 4,263,656 - Psyr_3580 0.50 -1.7 4,263,656 - Psyr_3580 0.50 +1.1 4,263,836 + Psyr_3580 0.63 -3.4 4,263,836 + Psyr_3580 0.63 -3.9 4,263,836 + Psyr_3580 0.63 +2.4 4,263,836 + Psyr_3580 0.63 +0.1 4,263,836 + Psyr_3580 0.63 +2.6 4,263,837 - Psyr_3580 0.63 +2.9 4,263,837 - Psyr_3580 0.63 -0.2 4,263,837 - Psyr_3580 0.63 -2.3 4,263,837 - Psyr_3580 0.63 +3.4 4,263,837 - Psyr_3580 0.63 +0.1 4,263,837 - Psyr_3580 0.63 +3.4 4,263,837 - Psyr_3580 0.63 +0.1 4,263,837 - Psyr_3580 0.63 -2.7 4,263,837 - Psyr_3580 0.63 +0.4 4,264,004 + Psyr_3580 0.75 +0.3 4,264,004 + Psyr_3580 0.75 -2.1 4,264,004 + Psyr_3580 0.75 +3.2 4,264,004 + Psyr_3580 0.75 -1.1 4,264,004 + Psyr_3580 0.75 +3.2 4,264,004 + Psyr_3580 0.75 -2.8 4,264,004 + Psyr_3580 0.75 -1.9 4,264,005 - Psyr_3580 0.75 -2.6 4,264,005 - Psyr_3580 0.75 +2.4 4,264,005 - Psyr_3580 0.75 -0.6 4,264,005 - Psyr_3580 0.75 -3.5 4,264,005 - Psyr_3580 0.75 -1.0 4,264,046 + Psyr_3580 0.78 -3.8 4,264,046 + Psyr_3580 0.78 -1.5 4,264,046 + Psyr_3580 0.78 +1.1 4,264,047 - Psyr_3580 0.78 -1.4 4,264,047 - Psyr_3580 0.78 +1.6 4,264,047 - Psyr_3580 0.78 -1.1 4,264,047 - Psyr_3580 0.78 -0.5 4,264,047 - Psyr_3580 0.78 +1.1 4,264,047 - Psyr_3580 0.78 -3.2 4,264,047 - Psyr_3580 0.78 -1.8 4,264,047 - Psyr_3580 0.78 +3.6 4,264,047 - Psyr_3580 0.78 -3.1 4,264,097 + Psyr_3580 0.82 +0.3 4,264,098 - Psyr_3580 0.82 +3.9 4,264,098 - Psyr_3580 0.82 -1.9 4,264,098 - Psyr_3580 0.82 +0.1 4,264,391 + +1.6 4,264,391 + -3.3 4,264,391 + +0.2 4,264,392 - -1.3 4,264,392 - +0.1 4,264,491 + -0.7 4,264,491 + -0.6 4,264,491 + -3.9 4,264,491 + +1.5 4,264,492 - +3.2 4,265,228 + Psyr_3581 0.49 -4.5 4,265,228 + Psyr_3581 0.49 +0.1 4,265,228 + Psyr_3581 0.49 -2.6 4,265,229 - Psyr_3581 0.49 +3.8 4,265,229 - Psyr_3581 0.49 +0.5
Or see this region's nucleotide sequence