Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3543 and Psyr_3544 are separated by 16 nucleotides Psyr_3544 and Psyr_3545 are separated by 101 nucleotides Psyr_3545 and Psyr_3546 are separated by 138 nucleotides
Psyr_3543: Psyr_3543 - transcriptional regulator, AraC family, at 4,220,788 to 4,221,675
_3543
Psyr_3544: Psyr_3544 - putative integral membrane protein, at 4,221,692 to 4,222,666
_3544
Psyr_3545: Psyr_3545 - Short-chain dehydrogenase/reductase SDR, at 4,222,768 to 4,223,511
_3545
Psyr_3546: Psyr_3546 - transcriptional regulator, AraC family, at 4,223,650 to 4,224,558
_3546
Position (kb)
4221
4222
4223 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4220.787 kb on + strand at 4220.905 kb on + strand, within Psyr_3543 at 4220.974 kb on + strand, within Psyr_3543 at 4220.974 kb on + strand, within Psyr_3543 at 4220.974 kb on + strand, within Psyr_3543 at 4220.975 kb on - strand, within Psyr_3543 at 4220.975 kb on - strand, within Psyr_3543 at 4220.989 kb on + strand, within Psyr_3543 at 4220.990 kb on - strand, within Psyr_3543 at 4221.089 kb on + strand, within Psyr_3543 at 4221.090 kb on - strand, within Psyr_3543 at 4221.370 kb on + strand, within Psyr_3543 at 4221.370 kb on + strand, within Psyr_3543 at 4221.371 kb on - strand, within Psyr_3543 at 4221.371 kb on - strand, within Psyr_3543 at 4221.371 kb on - strand, within Psyr_3543 at 4221.371 kb on - strand, within Psyr_3543 at 4221.434 kb on - strand, within Psyr_3543 at 4221.434 kb on - strand, within Psyr_3543 at 4221.550 kb on + strand, within Psyr_3543 at 4221.693 kb on + strand at 4221.693 kb on + strand at 4221.693 kb on + strand at 4221.693 kb on + strand at 4221.693 kb on + strand at 4221.693 kb on + strand at 4221.694 kb on - strand at 4221.694 kb on - strand at 4221.694 kb on - strand at 4221.694 kb on - strand at 4221.827 kb on - strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.832 kb on + strand, within Psyr_3544 at 4221.832 kb on + strand, within Psyr_3544 at 4221.832 kb on + strand, within Psyr_3544 at 4221.833 kb on - strand, within Psyr_3544 at 4221.835 kb on - strand, within Psyr_3544 at 4221.989 kb on - strand, within Psyr_3544 at 4222.007 kb on - strand, within Psyr_3544 at 4222.393 kb on - strand, within Psyr_3544 at 4222.412 kb on - strand, within Psyr_3544 at 4222.412 kb on - strand, within Psyr_3544 at 4222.647 kb on + strand at 4222.706 kb on + strand at 4222.738 kb on + strand at 4222.739 kb on - strand at 4222.746 kb on + strand at 4222.746 kb on + strand at 4222.747 kb on - strand at 4222.747 kb on - strand at 4222.978 kb on + strand, within Psyr_3545 at 4223.007 kb on + strand, within Psyr_3545 at 4223.007 kb on + strand, within Psyr_3545 at 4223.007 kb on + strand, within Psyr_3545 at 4223.008 kb on - strand, within Psyr_3545 at 4223.009 kb on + strand, within Psyr_3545 at 4223.010 kb on - strand, within Psyr_3545 at 4223.049 kb on - strand, within Psyr_3545 at 4223.417 kb on + strand, within Psyr_3545 at 4223.422 kb on - strand, within Psyr_3545 at 4223.422 kb on - strand, within Psyr_3545 at 4223.497 kb on + strand at 4223.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 4,220,787 + -0.8 4,220,905 + Psyr_3543 0.13 -2.9 4,220,974 + Psyr_3543 0.21 +1.1 4,220,974 + Psyr_3543 0.21 -3.6 4,220,974 + Psyr_3543 0.21 +2.6 4,220,975 - Psyr_3543 0.21 -0.4 4,220,975 - Psyr_3543 0.21 -0.9 4,220,989 + Psyr_3543 0.23 +1.6 4,220,990 - Psyr_3543 0.23 -2.2 4,221,089 + Psyr_3543 0.34 +0.1 4,221,090 - Psyr_3543 0.34 -2.1 4,221,370 + Psyr_3543 0.66 +3.1 4,221,370 + Psyr_3543 0.66 +0.1 4,221,371 - Psyr_3543 0.66 +2.6 4,221,371 - Psyr_3543 0.66 +2.4 4,221,371 - Psyr_3543 0.66 -0.8 4,221,371 - Psyr_3543 0.66 +0.6 4,221,434 - Psyr_3543 0.73 -2.6 4,221,434 - Psyr_3543 0.73 -0.4 4,221,550 + Psyr_3543 0.86 +1.1 4,221,693 + -4.1 4,221,693 + -1.3 4,221,693 + -1.5 4,221,693 + -1.3 4,221,693 + +2.1 4,221,693 + +1.6 4,221,694 - +2.9 4,221,694 - -2.8 4,221,694 - -2.1 4,221,694 - +1.1 4,221,827 - Psyr_3544 0.14 -0.9 4,221,828 + Psyr_3544 0.14 +3.1 4,221,828 + Psyr_3544 0.14 -0.0 4,221,828 + Psyr_3544 0.14 +2.1 4,221,828 + Psyr_3544 0.14 +0.1 4,221,828 + Psyr_3544 0.14 -0.6 4,221,828 + Psyr_3544 0.14 +3.4 4,221,828 + Psyr_3544 0.14 +2.4 4,221,828 + Psyr_3544 0.14 -1.5 4,221,828 + Psyr_3544 0.14 -1.0 4,221,828 + Psyr_3544 0.14 +2.0 4,221,829 - Psyr_3544 0.14 +0.1 4,221,829 - Psyr_3544 0.14 -2.3 4,221,829 - Psyr_3544 0.14 -0.7 4,221,829 - Psyr_3544 0.14 +3.2 4,221,829 - Psyr_3544 0.14 +3.9 4,221,829 - Psyr_3544 0.14 -1.9 4,221,829 - Psyr_3544 0.14 -0.3 4,221,832 + Psyr_3544 0.14 -2.4 4,221,832 + Psyr_3544 0.14 +2.9 4,221,832 + Psyr_3544 0.14 +1.9 4,221,833 - Psyr_3544 0.14 +0.1 4,221,835 - Psyr_3544 0.15 +4.4 4,221,989 - Psyr_3544 0.30 +2.4 4,222,007 - Psyr_3544 0.32 -1.0 4,222,393 - Psyr_3544 0.72 -1.6 4,222,412 - Psyr_3544 0.74 +3.6 4,222,412 - Psyr_3544 0.74 +3.5 4,222,647 + +3.5 4,222,706 + +0.1 4,222,738 + -1.9 4,222,739 - +3.2 4,222,746 + +1.6 4,222,746 + -2.3 4,222,747 - -1.8 4,222,747 - +0.1 4,222,978 + Psyr_3545 0.28 +2.1 4,223,007 + Psyr_3545 0.32 -1.9 4,223,007 + Psyr_3545 0.32 -0.9 4,223,007 + Psyr_3545 0.32 +0.1 4,223,008 - Psyr_3545 0.32 -1.3 4,223,009 + Psyr_3545 0.32 +2.1 4,223,010 - Psyr_3545 0.33 +0.6 4,223,049 - Psyr_3545 0.38 +0.1 4,223,417 + Psyr_3545 0.87 -1.3 4,223,422 - Psyr_3545 0.88 +0.1 4,223,422 - Psyr_3545 0.88 -2.3 4,223,497 + -0.2 4,223,498 - -3.0
Or see this region's nucleotide sequence