Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3454 and Psyr_3455 overlap by 11 nucleotides Psyr_3455 and Psyr_3456 are separated by 15 nucleotides Psyr_3456 and Psyr_3457 overlap by 8 nucleotides
Psyr_3454: Psyr_3454 - ATPase FliI/YscN, at 4,116,887 to 4,118,245
_3454
Psyr_3455: Psyr_3455 - Flagellar assembly protein FliH, at 4,118,235 to 4,119,053
_3455
Psyr_3456: Psyr_3456 - Flagellar motor switch protein FliG, at 4,119,069 to 4,120,085
_3456
Psyr_3457: Psyr_3457 - Flagellar FliF M-ring protein, at 4,120,078 to 4,121,862
_3457
Position (kb) 4119
4120
4121 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 4118.202 kb on + strand at 4118.202 kb on + strand at 4118.202 kb on + strand at 4118.203 kb on - strand at 4118.203 kb on - strand at 4118.203 kb on - strand at 4118.414 kb on + strand, within Psyr_3455 at 4118.415 kb on - strand, within Psyr_3455 at 4118.565 kb on + strand, within Psyr_3455 at 4118.566 kb on - strand, within Psyr_3455 at 4118.659 kb on + strand, within Psyr_3455 at 4118.659 kb on + strand, within Psyr_3455 at 4118.659 kb on + strand, within Psyr_3455 at 4118.660 kb on - strand, within Psyr_3455 at 4118.660 kb on - strand, within Psyr_3455 at 4118.660 kb on - strand, within Psyr_3455 at 4118.781 kb on + strand, within Psyr_3455 at 4118.781 kb on + strand, within Psyr_3455 at 4118.782 kb on - strand, within Psyr_3455 at 4119.070 kb on + strand at 4119.070 kb on + strand at 4119.071 kb on - strand at 4119.103 kb on - strand at 4119.125 kb on + strand at 4119.126 kb on - strand at 4119.288 kb on + strand, within Psyr_3456 at 4119.306 kb on + strand, within Psyr_3456 at 4119.307 kb on - strand, within Psyr_3456 at 4119.307 kb on - strand, within Psyr_3456 at 4119.307 kb on - strand, within Psyr_3456 at 4119.307 kb on - strand, within Psyr_3456 at 4119.570 kb on + strand, within Psyr_3456 at 4119.571 kb on - strand, within Psyr_3456 at 4119.665 kb on - strand, within Psyr_3456 at 4119.761 kb on + strand, within Psyr_3456 at 4119.762 kb on - strand, within Psyr_3456 at 4119.771 kb on + strand, within Psyr_3456 at 4119.772 kb on - strand, within Psyr_3456 at 4119.772 kb on - strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.842 kb on - strand, within Psyr_3456 at 4119.842 kb on - strand, within Psyr_3456 at 4119.842 kb on - strand, within Psyr_3456 at 4119.842 kb on - strand, within Psyr_3456 at 4119.842 kb on - strand, within Psyr_3456 at 4120.033 kb on - strand at 4120.172 kb on + strand at 4120.173 kb on - strand at 4120.200 kb on + strand at 4120.200 kb on + strand at 4120.200 kb on + strand at 4120.200 kb on + strand at 4120.201 kb on - strand at 4120.222 kb on + strand at 4120.342 kb on + strand, within Psyr_3457 at 4120.343 kb on - strand, within Psyr_3457 at 4120.418 kb on + strand, within Psyr_3457 at 4120.418 kb on + strand, within Psyr_3457 at 4120.418 kb on + strand, within Psyr_3457 at 4120.418 kb on + strand, within Psyr_3457 at 4121.031 kb on - strand, within Psyr_3457
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 4,118,202 + -1.6 4,118,202 + +3.9 4,118,202 + +2.8 4,118,203 - +0.7 4,118,203 - -0.3 4,118,203 - +0.4 4,118,414 + Psyr_3455 0.22 +3.9 4,118,415 - Psyr_3455 0.22 +1.8 4,118,565 + Psyr_3455 0.40 -0.5 4,118,566 - Psyr_3455 0.40 +4.2 4,118,659 + Psyr_3455 0.52 -1.2 4,118,659 + Psyr_3455 0.52 -1.5 4,118,659 + Psyr_3455 0.52 -0.6 4,118,660 - Psyr_3455 0.52 -0.6 4,118,660 - Psyr_3455 0.52 +1.6 4,118,660 - Psyr_3455 0.52 -0.3 4,118,781 + Psyr_3455 0.67 -2.9 4,118,781 + Psyr_3455 0.67 -1.2 4,118,782 - Psyr_3455 0.67 -1.6 4,119,070 + +1.2 4,119,070 + +1.6 4,119,071 - +3.0 4,119,103 - +3.0 4,119,125 + +4.3 4,119,126 - -0.9 4,119,288 + Psyr_3456 0.22 +4.2 4,119,306 + Psyr_3456 0.23 +2.0 4,119,307 - Psyr_3456 0.23 -0.0 4,119,307 - Psyr_3456 0.23 +3.0 4,119,307 - Psyr_3456 0.23 -1.1 4,119,307 - Psyr_3456 0.23 -0.3 4,119,570 + Psyr_3456 0.49 -2.9 4,119,571 - Psyr_3456 0.49 +3.0 4,119,665 - Psyr_3456 0.59 -1.0 4,119,761 + Psyr_3456 0.68 -2.5 4,119,762 - Psyr_3456 0.68 -1.1 4,119,771 + Psyr_3456 0.69 -0.0 4,119,772 - Psyr_3456 0.69 +0.8 4,119,772 - Psyr_3456 0.69 -1.0 4,119,841 + Psyr_3456 0.76 +3.0 4,119,841 + Psyr_3456 0.76 -0.0 4,119,841 + Psyr_3456 0.76 -5.1 4,119,841 + Psyr_3456 0.76 -1.0 4,119,841 + Psyr_3456 0.76 +2.3 4,119,841 + Psyr_3456 0.76 -4.9 4,119,841 + Psyr_3456 0.76 -4.7 4,119,842 - Psyr_3456 0.76 -0.3 4,119,842 - Psyr_3456 0.76 -2.2 4,119,842 - Psyr_3456 0.76 +0.1 4,119,842 - Psyr_3456 0.76 +3.6 4,119,842 - Psyr_3456 0.76 -2.5 4,120,033 - -1.0 4,120,172 + -2.0 4,120,173 - +0.6 4,120,200 + -2.3 4,120,200 + +1.6 4,120,200 + -2.5 4,120,200 + -3.0 4,120,201 - +3.9 4,120,222 + -3.9 4,120,342 + Psyr_3457 0.15 +0.3 4,120,343 - Psyr_3457 0.15 -1.7 4,120,418 + Psyr_3457 0.19 -0.3 4,120,418 + Psyr_3457 0.19 -1.6 4,120,418 + Psyr_3457 0.19 +3.0 4,120,418 + Psyr_3457 0.19 -0.7 4,121,031 - Psyr_3457 0.53 -0.2
Or see this region's nucleotide sequence