Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3244 and Psyr_3245 are separated by 86 nucleotides Psyr_3245 and Psyr_3246 are separated by 20 nucleotides
Psyr_3244: Psyr_3244 - Phospholipase D/Transphosphatidylase, at 3,884,704 to 3,886,047
_3244
Psyr_3245: Psyr_3245 - ATP-dependent DNA ligase LigD phosphoesterase module / ATP-dependent DNA ligase LigD polymerase module, at 3,886,134 to 3,888,734
_3245
Psyr_3246: Psyr_3246 - Ku, at 3,888,755 to 3,889,660
_3246
Position (kb)
3886
3887
3888
3889 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3885.136 kb on + strand, within Psyr_3244 at 3885.136 kb on + strand, within Psyr_3244 at 3885.136 kb on + strand, within Psyr_3244 at 3885.136 kb on + strand, within Psyr_3244 at 3885.137 kb on - strand, within Psyr_3244 at 3885.137 kb on - strand, within Psyr_3244 at 3885.137 kb on - strand, within Psyr_3244 at 3885.137 kb on - strand, within Psyr_3244 at 3885.137 kb on - strand, within Psyr_3244 at 3885.164 kb on - strand, within Psyr_3244 at 3885.180 kb on + strand, within Psyr_3244 at 3885.184 kb on + strand, within Psyr_3244 at 3885.260 kb on + strand, within Psyr_3244 at 3885.260 kb on + strand, within Psyr_3244 at 3885.261 kb on - strand, within Psyr_3244 at 3885.261 kb on - strand, within Psyr_3244 at 3885.508 kb on + strand, within Psyr_3244 at 3885.509 kb on - strand, within Psyr_3244 at 3885.509 kb on - strand, within Psyr_3244 at 3885.509 kb on - strand, within Psyr_3244 at 3885.655 kb on + strand, within Psyr_3244 at 3885.656 kb on - strand, within Psyr_3244 at 3885.661 kb on + strand, within Psyr_3244 at 3885.661 kb on + strand, within Psyr_3244 at 3885.790 kb on + strand, within Psyr_3244 at 3885.790 kb on + strand, within Psyr_3244 at 3885.943 kb on + strand at 3885.944 kb on - strand at 3885.944 kb on - strand at 3885.944 kb on - strand at 3885.971 kb on - strand at 3886.076 kb on + strand at 3886.076 kb on + strand at 3886.077 kb on - strand at 3886.100 kb on + strand at 3886.100 kb on + strand at 3886.101 kb on - strand at 3886.101 kb on - strand at 3886.101 kb on - strand at 3886.703 kb on + strand, within Psyr_3245 at 3886.704 kb on - strand, within Psyr_3245 at 3886.704 kb on - strand, within Psyr_3245 at 3886.704 kb on - strand, within Psyr_3245 at 3886.939 kb on + strand, within Psyr_3245 at 3887.020 kb on - strand, within Psyr_3245 at 3887.020 kb on - strand, within Psyr_3245 at 3887.313 kb on - strand, within Psyr_3245 at 3887.365 kb on + strand, within Psyr_3245 at 3887.365 kb on + strand, within Psyr_3245 at 3887.365 kb on + strand, within Psyr_3245 at 3887.366 kb on - strand, within Psyr_3245 at 3887.366 kb on - strand, within Psyr_3245 at 3887.366 kb on - strand, within Psyr_3245 at 3887.366 kb on - strand, within Psyr_3245 at 3887.442 kb on - strand, within Psyr_3245 at 3887.515 kb on + strand, within Psyr_3245 at 3887.516 kb on - strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.728 kb on + strand, within Psyr_3245 at 3887.729 kb on - strand, within Psyr_3245 at 3887.729 kb on - strand, within Psyr_3245 at 3887.729 kb on - strand, within Psyr_3245 at 3887.971 kb on + strand, within Psyr_3245 at 3887.972 kb on - strand, within Psyr_3245 at 3888.365 kb on - strand, within Psyr_3245 at 3888.433 kb on + strand, within Psyr_3245 at 3888.433 kb on + strand, within Psyr_3245 at 3888.712 kb on + strand at 3888.712 kb on + strand at 3888.712 kb on + strand at 3888.712 kb on + strand at 3888.713 kb on - strand at 3888.778 kb on + strand at 3888.778 kb on + strand at 3888.779 kb on - strand at 3888.779 kb on - strand at 3889.261 kb on + strand, within Psyr_3246 at 3889.293 kb on + strand, within Psyr_3246 at 3889.383 kb on - strand, within Psyr_3246 at 3889.446 kb on + strand, within Psyr_3246 at 3889.447 kb on - strand, within Psyr_3246 at 3889.527 kb on + strand, within Psyr_3246 at 3889.610 kb on + strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.613 kb on - strand at 3889.613 kb on - strand at 3889.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 3,885,136 + Psyr_3244 0.32 -0.1 3,885,136 + Psyr_3244 0.32 -0.1 3,885,136 + Psyr_3244 0.32 -3.0 3,885,136 + Psyr_3244 0.32 +3.1 3,885,137 - Psyr_3244 0.32 +0.3 3,885,137 - Psyr_3244 0.32 +3.7 3,885,137 - Psyr_3244 0.32 -1.5 3,885,137 - Psyr_3244 0.32 +3.2 3,885,137 - Psyr_3244 0.32 +0.9 3,885,164 - Psyr_3244 0.34 +2.2 3,885,180 + Psyr_3244 0.35 +2.5 3,885,184 + Psyr_3244 0.36 -4.4 3,885,260 + Psyr_3244 0.41 +0.0 3,885,260 + Psyr_3244 0.41 -1.0 3,885,261 - Psyr_3244 0.41 -0.1 3,885,261 - Psyr_3244 0.41 -3.7 3,885,508 + Psyr_3244 0.60 +2.2 3,885,509 - Psyr_3244 0.60 +2.9 3,885,509 - Psyr_3244 0.60 -0.1 3,885,509 - Psyr_3244 0.60 -1.3 3,885,655 + Psyr_3244 0.71 -1.6 3,885,656 - Psyr_3244 0.71 -0.6 3,885,661 + Psyr_3244 0.71 +3.6 3,885,661 + Psyr_3244 0.71 +2.2 3,885,790 + Psyr_3244 0.81 -0.5 3,885,790 + Psyr_3244 0.81 -1.2 3,885,943 + +2.2 3,885,944 - +1.9 3,885,944 - +3.2 3,885,944 - +3.1 3,885,971 - -1.9 3,886,076 + -2.2 3,886,076 + -0.9 3,886,077 - -1.2 3,886,100 + +2.2 3,886,100 + -0.2 3,886,101 - +2.2 3,886,101 - +4.7 3,886,101 - -2.1 3,886,703 + Psyr_3245 0.22 +0.8 3,886,704 - Psyr_3245 0.22 +3.1 3,886,704 - Psyr_3245 0.22 -1.2 3,886,704 - Psyr_3245 0.22 +2.9 3,886,939 + Psyr_3245 0.31 -0.9 3,887,020 - Psyr_3245 0.34 -1.7 3,887,020 - Psyr_3245 0.34 -0.8 3,887,313 - Psyr_3245 0.45 +2.9 3,887,365 + Psyr_3245 0.47 -2.4 3,887,365 + Psyr_3245 0.47 +1.9 3,887,365 + Psyr_3245 0.47 -0.1 3,887,366 - Psyr_3245 0.47 -0.5 3,887,366 - Psyr_3245 0.47 -0.2 3,887,366 - Psyr_3245 0.47 +1.9 3,887,366 - Psyr_3245 0.47 +3.6 3,887,442 - Psyr_3245 0.50 +3.4 3,887,515 + Psyr_3245 0.53 +0.1 3,887,516 - Psyr_3245 0.53 +1.9 3,887,725 + Psyr_3245 0.61 -2.8 3,887,725 + Psyr_3245 0.61 +0.5 3,887,725 + Psyr_3245 0.61 -5.1 3,887,725 + Psyr_3245 0.61 -2.1 3,887,728 + Psyr_3245 0.61 -1.6 3,887,729 - Psyr_3245 0.61 +0.9 3,887,729 - Psyr_3245 0.61 -0.9 3,887,729 - Psyr_3245 0.61 +2.7 3,887,971 + Psyr_3245 0.71 +0.2 3,887,972 - Psyr_3245 0.71 +2.9 3,888,365 - Psyr_3245 0.86 -1.2 3,888,433 + Psyr_3245 0.88 -0.1 3,888,433 + Psyr_3245 0.88 +2.2 3,888,712 + +0.0 3,888,712 + -1.9 3,888,712 + -2.7 3,888,712 + +1.5 3,888,713 - -0.1 3,888,778 + +1.9 3,888,778 + -1.7 3,888,779 - +0.5 3,888,779 - +2.7 3,889,261 + Psyr_3246 0.56 -0.1 3,889,293 + Psyr_3246 0.59 +0.4 3,889,383 - Psyr_3246 0.69 -3.0 3,889,446 + Psyr_3246 0.76 -0.1 3,889,447 - Psyr_3246 0.76 +3.2 3,889,527 + Psyr_3246 0.85 -1.5 3,889,610 + +1.0 3,889,612 + +0.1 3,889,612 + -0.4 3,889,612 + -1.3 3,889,612 + +2.9 3,889,612 + -2.5 3,889,612 + +0.1 3,889,612 + -0.8 3,889,613 - -3.1 3,889,613 - -3.8 3,889,613 - -0.4
Or see this region's nucleotide sequence