Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3045 and Psyr_3046 overlap by 4 nucleotides Psyr_3046 and Psyr_3047 are separated by 284 nucleotides Psyr_3047 and Psyr_3048 are separated by 30 nucleotides
Psyr_3045: Psyr_3045 - PqiB family protein, at 3,642,785 to 3,644,464
_3045
Psyr_3046: Psyr_3046 - Protein of unknown function DUF330, at 3,644,461 to 3,645,084
_3046
Psyr_3047: Psyr_3047 - Helix-turn-helix motif protein, at 3,645,369 to 3,645,818
_3047
Psyr_3048: Psyr_3048 - transcriptional regulator, LysR family, at 3,645,849 to 3,646,760
_3048
Position (kb)
3644
3645
3646 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3643.617 kb on + strand, within Psyr_3045 at 3643.618 kb on - strand, within Psyr_3045 at 3643.618 kb on - strand, within Psyr_3045 at 3643.667 kb on + strand, within Psyr_3045 at 3643.667 kb on + strand, within Psyr_3045 at 3643.667 kb on + strand, within Psyr_3045 at 3643.820 kb on + strand, within Psyr_3045 at 3643.820 kb on + strand, within Psyr_3045 at 3643.973 kb on + strand, within Psyr_3045 at 3643.973 kb on + strand, within Psyr_3045 at 3643.973 kb on + strand, within Psyr_3045 at 3643.974 kb on - strand, within Psyr_3045 at 3643.974 kb on - strand, within Psyr_3045 at 3643.974 kb on - strand, within Psyr_3045 at 3643.974 kb on - strand, within Psyr_3045 at 3643.974 kb on - strand, within Psyr_3045 at 3644.321 kb on - strand at 3644.486 kb on + strand at 3644.487 kb on - strand at 3644.487 kb on - strand at 3644.536 kb on + strand, within Psyr_3046 at 3644.536 kb on + strand, within Psyr_3046 at 3644.539 kb on + strand, within Psyr_3046 at 3644.539 kb on + strand, within Psyr_3046 at 3644.539 kb on + strand, within Psyr_3046 at 3644.539 kb on + strand, within Psyr_3046 at 3644.540 kb on - strand, within Psyr_3046 at 3644.540 kb on - strand, within Psyr_3046 at 3644.540 kb on - strand, within Psyr_3046 at 3644.860 kb on + strand, within Psyr_3046 at 3644.861 kb on - strand, within Psyr_3046 at 3644.861 kb on - strand, within Psyr_3046 at 3644.861 kb on - strand, within Psyr_3046 at 3645.090 kb on - strand at 3645.090 kb on - strand at 3645.105 kb on + strand at 3645.200 kb on + strand at 3645.219 kb on + strand at 3645.219 kb on + strand at 3645.220 kb on - strand at 3645.223 kb on + strand at 3645.224 kb on - strand at 3645.249 kb on + strand at 3645.249 kb on + strand at 3645.252 kb on + strand at 3645.252 kb on + strand at 3645.393 kb on - strand at 3645.393 kb on - strand at 3645.475 kb on - strand, within Psyr_3047 at 3645.475 kb on - strand, within Psyr_3047 at 3645.475 kb on - strand, within Psyr_3047 at 3645.507 kb on + strand, within Psyr_3047 at 3645.507 kb on + strand, within Psyr_3047 at 3645.507 kb on + strand, within Psyr_3047 at 3645.507 kb on + strand, within Psyr_3047 at 3645.508 kb on - strand, within Psyr_3047 at 3645.508 kb on - strand, within Psyr_3047 at 3645.546 kb on + strand, within Psyr_3047 at 3645.546 kb on + strand, within Psyr_3047 at 3645.546 kb on + strand, within Psyr_3047 at 3645.547 kb on - strand, within Psyr_3047 at 3645.547 kb on - strand, within Psyr_3047 at 3645.547 kb on - strand, within Psyr_3047 at 3645.547 kb on - strand, within Psyr_3047 at 3645.547 kb on - strand, within Psyr_3047 at 3645.547 kb on - strand, within Psyr_3047 at 3645.575 kb on + strand, within Psyr_3047 at 3645.576 kb on - strand, within Psyr_3047 at 3645.576 kb on - strand, within Psyr_3047 at 3645.592 kb on + strand, within Psyr_3047 at 3645.795 kb on + strand at 3645.795 kb on + strand at 3645.795 kb on + strand at 3645.795 kb on + strand at 3645.795 kb on + strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.817 kb on - strand at 3645.827 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 3,643,617 + Psyr_3045 0.50 -5.0 3,643,618 - Psyr_3045 0.50 +2.0 3,643,618 - Psyr_3045 0.50 +0.7 3,643,667 + Psyr_3045 0.53 +2.9 3,643,667 + Psyr_3045 0.53 +2.5 3,643,667 + Psyr_3045 0.53 +3.9 3,643,820 + Psyr_3045 0.62 +2.3 3,643,820 + Psyr_3045 0.62 -1.6 3,643,973 + Psyr_3045 0.71 +3.0 3,643,973 + Psyr_3045 0.71 +4.0 3,643,973 + Psyr_3045 0.71 +3.2 3,643,974 - Psyr_3045 0.71 +1.3 3,643,974 - Psyr_3045 0.71 -0.8 3,643,974 - Psyr_3045 0.71 +2.9 3,643,974 - Psyr_3045 0.71 +1.0 3,643,974 - Psyr_3045 0.71 +2.7 3,644,321 - -1.0 3,644,486 + -1.4 3,644,487 - +0.7 3,644,487 - +2.5 3,644,536 + Psyr_3046 0.12 +1.3 3,644,536 + Psyr_3046 0.12 -0.8 3,644,539 + Psyr_3046 0.12 +2.3 3,644,539 + Psyr_3046 0.12 +4.3 3,644,539 + Psyr_3046 0.12 +2.3 3,644,539 + Psyr_3046 0.12 +0.7 3,644,540 - Psyr_3046 0.13 -1.9 3,644,540 - Psyr_3046 0.13 +1.2 3,644,540 - Psyr_3046 0.13 -1.7 3,644,860 + Psyr_3046 0.64 -0.0 3,644,861 - Psyr_3046 0.64 +3.2 3,644,861 - Psyr_3046 0.64 +0.8 3,644,861 - Psyr_3046 0.64 -2.5 3,645,090 - +2.3 3,645,090 - +2.0 3,645,105 + +0.7 3,645,200 + +1.3 3,645,219 + -0.3 3,645,219 + -2.7 3,645,220 - -0.6 3,645,223 + +1.7 3,645,224 - +1.3 3,645,249 + +0.4 3,645,249 + +0.5 3,645,252 + -1.0 3,645,252 + -2.3 3,645,393 - +2.3 3,645,393 - +2.3 3,645,475 - Psyr_3047 0.24 -2.9 3,645,475 - Psyr_3047 0.24 +4.3 3,645,475 - Psyr_3047 0.24 -1.2 3,645,507 + Psyr_3047 0.31 -0.3 3,645,507 + Psyr_3047 0.31 -0.3 3,645,507 + Psyr_3047 0.31 +1.5 3,645,507 + Psyr_3047 0.31 +0.7 3,645,508 - Psyr_3047 0.31 -1.3 3,645,508 - Psyr_3047 0.31 +3.5 3,645,546 + Psyr_3047 0.39 -0.3 3,645,546 + Psyr_3047 0.39 -2.5 3,645,546 + Psyr_3047 0.39 -3.4 3,645,547 - Psyr_3047 0.40 -0.3 3,645,547 - Psyr_3047 0.40 +1.7 3,645,547 - Psyr_3047 0.40 -4.5 3,645,547 - Psyr_3047 0.40 -1.3 3,645,547 - Psyr_3047 0.40 +0.6 3,645,547 - Psyr_3047 0.40 -3.0 3,645,575 + Psyr_3047 0.46 -2.9 3,645,576 - Psyr_3047 0.46 -1.9 3,645,576 - Psyr_3047 0.46 +2.9 3,645,592 + Psyr_3047 0.50 -0.3 3,645,795 + -1.1 3,645,795 + -0.3 3,645,795 + +1.0 3,645,795 + +3.0 3,645,795 + +0.7 3,645,796 - +0.7 3,645,796 - -0.4 3,645,796 - -1.3 3,645,796 - +1.0 3,645,796 - +3.0 3,645,796 - +1.3 3,645,796 - +2.6 3,645,796 - +0.4 3,645,817 - -0.9 3,645,827 + +3.2
Or see this region's nucleotide sequence