Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3037 and Psyr_3038 are separated by 113 nucleotides Psyr_3038 and Psyr_3039 are separated by 153 nucleotides Psyr_3039 and Psyr_3040 are separated by 93 nucleotides Psyr_3040 and Psyr_3041 are separated by 267 nucleotides
Psyr_3037: Psyr_3037 - membrane protein, putative, at 3,636,983 to 3,638,344
_3037
Psyr_3038: Psyr_3038 - conserved hypothetical protein, at 3,638,458 to 3,638,712
_3038
Psyr_3039: Psyr_3039 - protein of unknown function DUF883, at 3,638,866 to 3,639,183
_3039
Psyr_3040: Psyr_3040 - conserved hypothetical protein, at 3,639,277 to 3,639,618
_3040
Psyr_3041: Psyr_3041 - Bacillolysin, at 3,639,886 to 3,640,956
_3041
Position (kb)
3639
3640 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3638.409 kb on + strand at 3638.410 kb on - strand at 3638.459 kb on + strand at 3638.460 kb on - strand at 3638.460 kb on - strand at 3638.510 kb on + strand, within Psyr_3038 at 3638.723 kb on + strand at 3638.746 kb on + strand at 3638.746 kb on + strand at 3638.747 kb on - strand at 3638.747 kb on - strand at 3638.859 kb on + strand at 3638.859 kb on + strand at 3638.860 kb on - strand at 3638.860 kb on - strand at 3638.874 kb on + strand at 3638.875 kb on - strand at 3638.875 kb on - strand at 3639.100 kb on + strand, within Psyr_3039 at 3639.100 kb on + strand, within Psyr_3039 at 3639.101 kb on - strand, within Psyr_3039 at 3639.101 kb on - strand, within Psyr_3039 at 3639.133 kb on - strand, within Psyr_3039 at 3639.155 kb on + strand at 3639.155 kb on + strand at 3639.259 kb on + strand at 3639.259 kb on + strand at 3639.260 kb on - strand at 3639.380 kb on + strand, within Psyr_3040 at 3639.394 kb on + strand, within Psyr_3040 at 3639.394 kb on + strand, within Psyr_3040 at 3639.434 kb on + strand, within Psyr_3040 at 3639.435 kb on - strand, within Psyr_3040 at 3639.620 kb on + strand at 3639.620 kb on + strand at 3639.620 kb on + strand at 3639.620 kb on + strand at 3639.620 kb on + strand at 3639.621 kb on - strand at 3639.621 kb on - strand at 3639.621 kb on - strand at 3639.621 kb on - strand at 3639.653 kb on + strand at 3639.654 kb on - strand at 3639.654 kb on - strand at 3639.654 kb on - strand at 3639.722 kb on - strand at 3639.780 kb on + strand at 3639.780 kb on + strand at 3639.781 kb on - strand at 3639.800 kb on + strand at 3639.802 kb on + strand at 3639.802 kb on + strand at 3639.803 kb on - strand at 3639.869 kb on + strand at 3639.870 kb on + strand at 3639.870 kb on - strand at 3639.872 kb on + strand at 3639.873 kb on - strand at 3639.873 kb on - strand at 3639.873 kb on - strand at 3639.928 kb on + strand at 3639.928 kb on + strand at 3639.929 kb on - strand at 3639.929 kb on - strand at 3639.929 kb on - strand at 3639.929 kb on - strand at 3639.942 kb on + strand at 3640.183 kb on + strand, within Psyr_3041 at 3640.204 kb on + strand, within Psyr_3041 at 3640.205 kb on - strand, within Psyr_3041 at 3640.205 kb on - strand, within Psyr_3041 at 3640.213 kb on + strand, within Psyr_3041 at 3640.213 kb on + strand, within Psyr_3041 at 3640.310 kb on + strand, within Psyr_3041 at 3640.311 kb on - strand, within Psyr_3041 at 3640.311 kb on - strand, within Psyr_3041 at 3640.311 kb on - strand, within Psyr_3041 at 3640.351 kb on + strand, within Psyr_3041
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 3,638,409 + +2.0 3,638,410 - +1.0 3,638,459 + -0.3 3,638,460 - +0.7 3,638,460 - +0.8 3,638,510 + Psyr_3038 0.20 -2.9 3,638,723 + +3.9 3,638,746 + -0.5 3,638,746 + +2.5 3,638,747 - +0.6 3,638,747 - +3.2 3,638,859 + +0.7 3,638,859 + +0.3 3,638,860 - +1.7 3,638,860 - -3.5 3,638,874 + -0.9 3,638,875 - +4.3 3,638,875 - +2.7 3,639,100 + Psyr_3039 0.74 -0.1 3,639,100 + Psyr_3039 0.74 +0.1 3,639,101 - Psyr_3039 0.74 +3.2 3,639,101 - Psyr_3039 0.74 -1.8 3,639,133 - Psyr_3039 0.84 +2.5 3,639,155 + -1.0 3,639,155 + -0.7 3,639,259 + -4.1 3,639,259 + -3.9 3,639,260 - +2.7 3,639,380 + Psyr_3040 0.30 -2.4 3,639,394 + Psyr_3040 0.34 -3.1 3,639,394 + Psyr_3040 0.34 +2.5 3,639,434 + Psyr_3040 0.46 -0.3 3,639,435 - Psyr_3040 0.46 -1.9 3,639,620 + -0.3 3,639,620 + +2.5 3,639,620 + -1.1 3,639,620 + -0.7 3,639,620 + +2.9 3,639,621 - +0.7 3,639,621 - +2.0 3,639,621 - +3.8 3,639,621 - +2.4 3,639,653 + -3.3 3,639,654 - -0.6 3,639,654 - +0.7 3,639,654 - -0.7 3,639,722 - -1.5 3,639,780 + -2.4 3,639,780 + -1.1 3,639,781 - -0.4 3,639,800 + -1.7 3,639,802 + +2.0 3,639,802 + +0.7 3,639,803 - +2.9 3,639,869 + -1.5 3,639,870 + +0.7 3,639,870 - -1.5 3,639,872 + +1.3 3,639,873 - +1.7 3,639,873 - -0.4 3,639,873 - -1.9 3,639,928 + -4.4 3,639,928 + -3.1 3,639,929 - -1.4 3,639,929 - +1.3 3,639,929 - +1.3 3,639,929 - -1.1 3,639,942 + -4.2 3,640,183 + Psyr_3041 0.28 -3.8 3,640,204 + Psyr_3041 0.30 -1.9 3,640,205 - Psyr_3041 0.30 -0.9 3,640,205 - Psyr_3041 0.30 -0.4 3,640,213 + Psyr_3041 0.31 -2.1 3,640,213 + Psyr_3041 0.31 -2.3 3,640,310 + Psyr_3041 0.40 -2.9 3,640,311 - Psyr_3041 0.40 -3.3 3,640,311 - Psyr_3041 0.40 +0.7 3,640,311 - Psyr_3041 0.40 -2.3 3,640,351 + Psyr_3041 0.43 -3.1
Or see this region's nucleotide sequence