Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2998 and Psyr_2999 are separated by 13 nucleotides Psyr_2999 and Psyr_3000 overlap by 4 nucleotides Psyr_3000 and Psyr_3001 overlap by 32 nucleotides
Psyr_2998: Psyr_2998 - conserved hypothetical protein, at 3,594,701 to 3,595,714
_2998
Psyr_2999: Psyr_2999 - LmbE-like protein, at 3,595,728 to 3,596,501
_2999
Psyr_3000: Psyr_3000 - methyltransferase, putative, at 3,596,498 to 3,597,124
_3000
Psyr_3001: Psyr_3001 - Glycosyl transferase, family 2, at 3,597,093 to 3,597,746
_3001
Position (kb) 3595
3596
3597 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 3594.778 kb on - strand at 3594.778 kb on - strand at 3594.778 kb on - strand at 3594.896 kb on + strand, within Psyr_2998 at 3594.897 kb on - strand, within Psyr_2998 at 3595.009 kb on + strand, within Psyr_2998 at 3595.263 kb on - strand, within Psyr_2998 at 3595.511 kb on + strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.575 kb on - strand, within Psyr_2998 at 3595.720 kb on + strand at 3595.772 kb on + strand at 3595.773 kb on - strand at 3595.894 kb on + strand, within Psyr_2999 at 3595.894 kb on + strand, within Psyr_2999 at 3595.894 kb on + strand, within Psyr_2999 at 3595.895 kb on - strand, within Psyr_2999 at 3595.895 kb on - strand, within Psyr_2999 at 3595.970 kb on + strand, within Psyr_2999 at 3595.971 kb on - strand, within Psyr_2999 at 3596.375 kb on + strand, within Psyr_2999 at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.517 kb on - strand at 3596.517 kb on - strand at 3596.573 kb on + strand, within Psyr_3000 at 3596.843 kb on + strand, within Psyr_3000 at 3596.847 kb on - strand, within Psyr_3000 at 3597.134 kb on - strand at 3597.134 kb on - strand at 3597.134 kb on - strand at 3597.359 kb on + strand, within Psyr_3001 at 3597.489 kb on + strand, within Psyr_3001 at 3597.490 kb on - strand, within Psyr_3001
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 3,594,778 - +0.0 3,594,778 - -3.4 3,594,778 - +2.2 3,594,896 + Psyr_2998 0.19 +0.0 3,594,897 - Psyr_2998 0.19 -1.7 3,595,009 + Psyr_2998 0.30 +0.3 3,595,263 - Psyr_2998 0.55 +2.8 3,595,511 + Psyr_2998 0.80 -2.6 3,595,512 - Psyr_2998 0.80 +0.7 3,595,512 - Psyr_2998 0.80 -0.4 3,595,512 - Psyr_2998 0.80 +4.0 3,595,512 - Psyr_2998 0.80 +0.4 3,595,512 - Psyr_2998 0.80 +3.1 3,595,575 - Psyr_2998 0.86 -4.6 3,595,720 + -2.0 3,595,772 + +3.7 3,595,773 - +4.0 3,595,894 + Psyr_2999 0.21 +2.0 3,595,894 + Psyr_2999 0.21 -1.1 3,595,894 + Psyr_2999 0.21 -2.2 3,595,895 - Psyr_2999 0.22 +3.0 3,595,895 - Psyr_2999 0.22 -3.9 3,595,970 + Psyr_2999 0.31 +2.4 3,595,971 - Psyr_2999 0.31 -0.9 3,596,375 + Psyr_2999 0.84 -1.7 3,596,516 + -0.4 3,596,516 + +2.8 3,596,516 + +0.1 3,596,516 + -0.4 3,596,516 + +1.6 3,596,516 + -3.3 3,596,517 - +2.5 3,596,517 - -0.9 3,596,573 + Psyr_3000 0.12 -3.2 3,596,843 + Psyr_3000 0.55 +0.2 3,596,847 - Psyr_3000 0.56 +0.5 3,597,134 - -3.9 3,597,134 - +4.0 3,597,134 - -0.9 3,597,359 + Psyr_3001 0.41 +0.7 3,597,489 + Psyr_3001 0.61 +3.6 3,597,490 - Psyr_3001 0.61 -0.9
Or see this region's nucleotide sequence